Characterization of the rDNA repeat units in the MitchellPetunia genome.

Plant molecular biology ·Vol. 2 ·No. 2 ·1983-03-00 ·Pages 57-65

Waldron J, Dunsmuir P, Bedbrook J

Abstract

The principal rDNA repeating unit in the MitchellPetunia genome has a length of 8.5 kb. In addition there is a major variant of length 9.7 kb, and two minor variants of 9.3 kb and 10.4 kb. The size heterogeneity of the rDNA repeating units results from length differences in the non-transcribed spacer regions. These differences may reflect simple insertions into the non-transcribed spacer region of the major 'short' repeat; however, additional sequence changes have occurred since the 'short' repeat is characterized by restriction endonuclease cleavage sites which are absent in the longer variant units.

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Waldron J

CSIRO Division of Plant Industry, P. O. Box 1600, 2601, Canberra City, A.C.T., Australia.

Dunsmuir P

Bedbrook J

References (18)

18 references, click to expand

The isolation of structural genes from libraries of eucaryotic DNA.
Cell. 1978 Oct;15(2):687-701 PMID: 719759
Cloning and characterization of ribosomal RNA genes from wheat and barley.
Nucleic Acids Res. 1979 Dec 11;7(7):1869-85 PMID: 537913
A membrane-filter technique for the detection of complementary DNA.
Biochem Biophys Res Commun. 1966 Jun 13;23(5):641-6 PMID: 5963888
Construction of coliphage lambda Charon vectors with BamHI cloning sites.
Gene. 1980 Dec;12(3-4):301-9 PMID: 6265323
Screening lambdagt recombinant clones by hybridization to single plaques in situ.
Science. 1977 Apr 8;196(4286):180-2 PMID: 322279
Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids.
Cell. 1977 Nov;12(3):721-32 PMID: 922889
Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels.
J Mol Biol. 1977 Feb 15;110(1):119-46 PMID: 845942
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
Methylmercury as a reversible denaturing agent for agarose gel electrophoresis.
Anal Biochem. 1976 Jan;70(1):75-85 PMID: 1259158
Characterisation of the genes for ribosomal RNA in flax.
Nucleic Acids Res. 1981 Mar 25;9(6):1301-9 PMID: 6262724
A comparison of the ribosomal DNA's of Xenopus laevis and Xenopus mulleri: the evolution of tandem genes.
J Mol Biol. 1972 Jan 14;63(1):57-73 PMID: 5016971
The organization of a nuclear DNA sequence from a higher plant: molecular cloning and characterization of soybean ribosomal DNA.
Gene. 1979 Nov;7(3-4):317-34 PMID: 160361
Isolation of the bacteriophage lambda A-gene protein.
Proc Natl Acad Sci U S A. 1975 Feb;72(2):581-5 PMID: 1054839
The use of R-looping for structural gene identification and mRNA purification.
Nucleic Acids Res. 1979 Jun 11;6(7):2483-97 PMID: 379820
Repeated genes in eukaryotes.
Annu Rev Biochem. 1980;49:727-64 PMID: 6996571
Purification and restriction endonuclease mapping of soybean 18 S and 25 S ribosomal RNA genes.
Planta. 1979 Sep;146(4):467-73 PMID: 24318255
The wheat ribosomal DNA spacer region: Its structure and variation in populations and among species.
Theor Appl Genet. 1982 Dec;63(4):337-48 PMID: 24270871
Polyribosomes from peas: an improved method for their isolation in the absence of ribonuclease inhibitors.
Plant Physiol. 1972 Nov;50(5):581-4 PMID: 16658221

Article Info

Journal

Plant molecular biology

Abbr.

Plant Mol Biol

ISSN

0167-4412

DOI

10.1007/BF01595166

Published

1983-03-00

Pages

57-65

Language

English

Region

Netherlands

NLM ID

9106343

External Links

PubMed source DOI: 10.1007/BF01595166 View journal details

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Characterization of the rDNA repeat units in the MitchellPetunia genome.

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