Home LiteratureArticle Details
PMID: 2436147 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Discrete high molecular weight RNA transcribed from the long interspersed repetitive element L1Md.

Nucleic acids research ·Vol. 15 ·No. 6 ·1987-03-25 ·Pages 2581-92

Dudley JP

Abstract

The repetitive element LINE (L1) previously has been shown to contain two long open reading frames which are overlapping and out-of-frame similar to those found in retroviruses (1). In rodents and in human cells, these repeats appear to be transcribed into a heterogeneous population of RNAs in most cell types (2,3,4). No discrete transcript has been reported which is likely to be a mRNA for the open reading frames in rodent cells. In this paper, a discrete RNA species of approximately 8 kb has been identified in most murine lymphoid cells examined. This RNA is cytoplasmic and binds to oligo (dT) cellulose columns. Hybridization with labeled probes indicates that the transcript is of the same strandedness as the open reading frames. These results are consistent with proposals that L1Md is a retroposon with protein-encoding function.

MeSH Terms
Animals Cell Line Cloning, Molecular DNA Restriction Enzymes Humans Nucleic Acid Hybridization RNA/genetics Repetitive Sequences, Nucleic Acid Transcription, Genetic
Chemicals
RNA DNA Restriction Enzymes
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Dudley J P
References (31)
31 references, click to expand
  1. Phenotypic variation in clonal Abelson virus lymphoma cells.
    J Immunol. 1985 Feb;134(2):1268-75 PMID: 2856928
  2. Size heterogeneity in the 3' end of dihydrofolate reductase messenger RNAs in mouse cells.
    Cell. 1980 Nov;22(2 Pt 2):361-70 PMID: 7448865
  3. Reverse transcriptase activity and Ty RNA are associated with virus-like particles in yeast.
    Nature. 1985 Dec 12-18;318(6046):583-6 PMID: 2415827
  4. Transcripts homologous to a long repeated DNA element in the human genome.
    J Biol Chem. 1984 Jan 25;259(2):1218-25 PMID: 6198321
  5. The origin and evolution of retroposons.
    Int Rev Cytol. 1985;93:187-279 PMID: 2409043
  6. Retrovirus-like particles containing RNA homologous to the transposable element copia in Drosophila melanogaster.
    Nature. 1983 Mar 10;302(5904):119-24 PMID: 6186922
  7. Nucleotide sequence of a yeast Ty element: evidence for an unusual mechanism of gene expression.
    Proc Natl Acad Sci U S A. 1985 May;82(9):2829-33 PMID: 2581255
  8. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
    Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201-5 PMID: 6159641
  9. Molecular cloning and characterization of mouse mammary tumor proviruses from a T-cell lymphoma.
    J Virol. 1986 Jan;57(1):385-8 PMID: 2867232
  10. Purification and translation of murine mammary tumor virus mRNA's.
    J Virol. 1981 Jul;39(1):207-18 PMID: 6268810
  11. Discrete and heterogeneous high molecular weight RNAs complementary to a long dispersed repeat family (a possible transposon) of human DNA.
    J Mol Biol. 1983 Apr 5;165(2):257-86 PMID: 6188844
  12. Amplification and novel locations of endogenous mouse mammary tumor virus genomes in mouse T-cell lymphomas.
    J Virol. 1984 Jan;49(1):92-101 PMID: 6317898
  13. Inhibition of intractable nucleases with ribonucleoside--vanadyl complexes: isolation of messenger ribonucleic acid from resting lymphocytes.
    Biochemistry. 1979 Nov 13;18(23):5143-9 PMID: 497174
  14. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.
    Gene. 1982 Oct;19(3):259-68 PMID: 6295879
  15. Dispersal process associated with the L1 family of interspersed repetitive DNA sequences.
    J Mol Biol. 1984 Oct 5;178(4):795-813 PMID: 6492166
  16. The sequence of a large L1Md element reveals a tandemly repeated 5' end and several features found in retrotransposons.
    Mol Cell Biol. 1986 Jan;6(1):168-82 PMID: 3023821
  17. Repetitive sequence transcripts in development.
    Nature. 1982 Jun 24;297(5868):633-5 PMID: 6178032
  18. Expression of a cytoplasmic LINE-1 transcript is regulated in a human teratocarcinoma cell line.
    Proc Natl Acad Sci U S A. 1985 Sep;82(18):6050-4 PMID: 2412228
  19. Magnesium precipitation of ribonucleoprotein complexes. Expedient techniques for the isolation of undergraded polysomes and messenger ribonucleic acid.
    Biochemistry. 1974 Aug 13;13(17):3606-15 PMID: 4858491
  20. A family of long reiterated DNA sequences, one copy of which is next to the human beta globin gene.
    Nucleic Acids Res. 1980 Dec 20;8(24):6113-28 PMID: 6258162
  21. Some ultrastructural characteristics of a series of primary and transplanted plasma-cell tumors of the mouse.
    J Natl Cancer Inst. 1961 May;26:1221-67 PMID: 13719477
  22. Association of two different repetitive DNA elements near immunoglobulin light chain genes.
    Nucleic Acids Res. 1983 Mar 25;11(6):1803-17 PMID: 6300779
  23. Repetitive sequences in eukaryotic DNA and their expression.
    Annu Rev Biochem. 1982;51:813-44 PMID: 7051966
  24. Activation of a novel KpnI transcript by downstream integration of a human T-lymphotropic virus type I provirus.
    J Biol Chem. 1986 Apr 5;261(10):4615-9 PMID: 3007468
  25. Target sites for the transposition of rat long interspersed repeated DNA elements (LINEs) are not random.
    Nucleic Acids Res. 1986 May 12;14(9):3717-27 PMID: 3012480
  26. Structural differences in a single gene encoding the V kappa Ser group of light chains explain the existence of two mouse light-chain genetic markers.
    Proc Natl Acad Sci U S A. 1986 Dec;83(23):9134-8 PMID: 3097643
  27. Activation of the c-mos oncogene in a mouse plasmacytoma by insertion of an endogenous intracisternal A-particle genome.
    Proc Natl Acad Sci U S A. 1983 Dec;80(23):7118-22 PMID: 6316344
  28. A large interspersed repeat found in mouse DNA contains a long open reading frame that evolves as if it encodes a protein.
    Proc Natl Acad Sci U S A. 1984 Apr;81(8):2308-12 PMID: 6326120
  29. Rearrangement of the oncogene c-mos in mouse myeloma NSI and hybridomas.
    Nature. 1983 Dec 22-1984 Jan 4;306(5945):797-9 PMID: 6656883
  30. Mouse myelomas and lymphomas in culture.
    Exp Cell Res. 1970 Apr;60(1):61-77 PMID: 5439579
  31. The distribution of interspersed repeats is nonuniform and conserved in the mouse and human genomes.
    Proc Natl Acad Sci U S A. 1983 Apr;80(7):1816-20 PMID: 6572942
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/15.6.2581
Published
1987-03-25
Pages
2581-92
Language
English
Region
England
NLM ID
0411011
PMCID
PMC340670
Subset
IM
Grants
NCI NIH HHS · R01 CA34780 · United States
External Links
PubMed source DOI: 10.1093/nar/15.6.2581 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2