A transposable epitope of HLA-B7, B40 molecules.
The monoclonal antibody MB40.2 defines a novel subtype of HLA-B40 that is expressed by the Sweig cell line. This molecule, called HLA-B40, lacks an antigenic determinant that is common to HLA-B7 and the HLA-Bw60 subtype of HLA-B40. Genes encoding HLA-B40 and HLA-Bw60 have now been isolated and the amino acid sequences of these proteins compared with other HLA-B locus molecules. These results show that HLA-B40 is a unique protein which differs from HLA-Bw60 by eight amino acid substitutions. Comparison of the sequences for HLA-B40, -Bw60, and -B7 localizes the MB40.2 epitope to a cluster of three substitutions at positions 177, 178, and 180 at the end of the alpha 2 domain. Gene conversion or reciprocal recombination are postulated to have transferred this cluster of substitutions, and their associated epitope, during the evolution of HLA-B locus genes. The epitope may consist of an alpha helical segment which is exclusively found on MB40.2-positive molecules.
28 references, click to expand
-
The binding of monoclonal antibodies to cell surface molecules. Quantitative analysis of the reactions and cross-reactions of an antibody (MB40.3) with four HLA-B molecules.J Biol Chem. 1984 Nov 10;259(21):13077-83 PMID: 6333424
-
Interferon-dependent induction of mRNA for the major histocompatibility antigens in human fibroblasts and lymphoblastoid cells.Proc Natl Acad Sci U S A. 1982 May;79(10):3082-6 PMID: 6179076
-
The complete primary structure of HLA-Bw58.J Biol Chem. 1985 Oct 5;260(22):11924-33 PMID: 2995352
-
A monoclonal antibody directed against the HLA-Bw6 epitope.J Immunol. 1982 Jun;128(6):2804-6 PMID: 6176653
-
A simple method for displaying the hydropathic character of a protein.J Mol Biol. 1982 May 5;157(1):105-32 PMID: 7108955
-
Structural analysis of an HLA-B7 antigen variant detected by cytotoxic T lymphocytes.J Immunol. 1984 Aug;133(2):816-21 PMID: 6429245
-
Gene conversion-like mechanisms may generate polymorphism in human class I genes.EMBO J. 1986 Mar;5(3):547-52 PMID: 3011411
-
Human major histocompatibility complex class I antigens: residues 61-83 of the HLA-B7 heavy chain specify an alloreactive site.Proc Natl Acad Sci U S A. 1985 Jan;82(2):539-42 PMID: 3881768
-
Primary structure of papain-solubilized human histocompatibility antigen HLA-B40 (-Bw60). An outline of alloantigenic determinants.Biochemistry. 1983 Aug 2;22(16):3961-9 PMID: 6193808
-
Biochemical transfer of single-copy eucaryotic genes using total cellular DNA as donor.Cell. 1978 Jul;14(3):725-31 PMID: 210957
-
HLA-B27 antigenicity: antibodies against the chemically synthesized 63-84 peptide from HLA-B27.1 display alloantigenic specificity and discriminate among HLA-B27 subtypes.J Immunol. 1986 Aug 1;137(3):904-10 PMID: 2424988
-
DNA sequence of the coding region of the HLA-B44 gene.Immunogenetics. 1986;23(6):396-400 PMID: 3459708
-
Primary structure of papain-solubilized human histocompatibility antigen HLA-B27.Biochemistry. 1985 Mar 26;24(7):1733-41 PMID: 2408663
-
Complete amino acid sequence of a papain-solubilized human histocompatibility antigen, HLA-B7. 2. Sequence determination and search for homologies.Biochemistry. 1979 Dec 11;18(25):5711-20 PMID: 518865
-
Changes in conformation with loss of alloantigenic determinants of a histocompatibility antigen (HLA-B7) induced by monoclonal antibodies.J Immunol. 1984 Jun;132(6):2975-83 PMID: 6202768
-
Amino acid residues 56 to 69 of HLA-A2 specify an antigenic determinant shared by HLA-A2 and HLA-B17.J Immunol. 1986 Jul 1;137(1):217-22 PMID: 2423602
-
Exon shuffling in vivo can generate novel HLA class I molecules.EMBO J. 1985 Nov;4(11):2849-54 PMID: 3877632
-
Comparative structural analysis of HLA-A2 antigens distinguishable by cytotoxic T lymphocytes. II. Variant DK1: evidence for a discrete CTL recognition region.J Immunol. 1983 Apr;130(4):1856-62 PMID: 6601143
-
The primary structure of HLA-A32 suggests a region involved in formation of the Bw4/Bw6 epitopes.J Immunol. 1986 Dec 1;137(11):3671-4 PMID: 2431040
-
The monoclonal antibody CR11-351 discriminates HLA-A2 variants identified by T cells.Immunogenetics. 1983;18(1):23-35 PMID: 6190744
-
Monoclonal antibodies against two separate alloantigenic sites of HLA-B40.Immunogenetics. 1981;13(6):509-27 PMID: 7026428
-
HLA-B locus polymorphism: studies with a specific hybridization probe.Proc Natl Acad Sci U S A. 1985 Dec;82(24):8614-8 PMID: 3001712
-
Further studies on the epitopes of HLA-B7 defined by murine monoclonal antibodies.Hum Immunol. 1986 Jan;15(1):44-67 PMID: 2419285
-
Murine major histocompatibility complex class-I mutants: molecular analysis and structure-function implications.Annu Rev Immunol. 1986;4:471-502 PMID: 3518748
-
Antigenic determinants of the HLA-B7 molecule; Bw6- and B7-specific determinants are spatially separate.Immunogenetics. 1983;18(1):1-16 PMID: 6190743
-
Prediction of the secondary structure of proteins from their amino acid sequence.Adv Enzymol Relat Areas Mol Biol. 1978;47:45-148 PMID: 364941
-
Monoclonal antibody to a human histocompatibility alloantigen, HLA-A2.Nature. 1978 Nov 23;276(5686):397-9 PMID: 714164
-
Monoclonal antibodies prepared against Dictyostelium actin: characterization and interactions with actin.J Cell Biol. 1984 Jul;99(1 Pt 1):287-95 PMID: 6203918
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