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PMID: 2447290 Published · ppublish English Journal Article

A C-terminal domain in the avian sarcoma-leukosis virus pol gene product is not essential for viral replication.

Journal of virology ·Vol. 62 ·No. 2 ·1988-02-00 ·Pages 528-33

Katz RA, Skalka AM

Abstract

The virion proteins encoded by the avian retroviral pol gene (reverse transcriptase and endonuclease) are formed by the proteolytic processing of a gag-pol fusion protein precursor. Recent studies have predicted that the avian sarcoma-leukosis virus pol precursor protein undergoes a previously undetected processing event resulting in the formation of common C termini for the endonuclease (pp32) and the beta subunit of reverse transcriptase (F. Alexander, J. Leis, D. A. Soltis, R. M. Crowl, W. Danho, M. S. Poonian, Y.-C. E. Pan, and A. M. Skalka, J. Virol. 61:534-542, 1987; D. Grandgenett, T. Quinn, P. J. Hippenmeyer, and S. Oroszlan, J. Biol. Chem. 260:8243-8249, 1985). This processing event removes 37 amino acids, thus defining a new pol domain. In this report, we present evidence that this C-terminal domain is translated as part of the gag-pol precursor but is not required for replication of the virus in tissue culture cells.

MeSH Terms
Amino Acid Sequence Animals Avian Leukosis Virus/genetics,physiology Avian Sarcoma Viruses/genetics,physiology Base Sequence Chick Embryo Codon/genetics Endodeoxyribonucleases/genetics,metabolism Fibroblasts Genes, Viral Molecular Sequence Data Mutation Protein Biosynthesis Protein Precursors/genetics,metabolism RNA-Directed DNA Polymerase/genetics,metabolism Retroviridae Proteins/genetics,metabolism Transfection Virus Replication
Chemicals
Codon Protein Precursors Retroviridae Proteins avian retrovirus proteins RNA-Directed DNA Polymerase Endodeoxyribonucleases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Katz R A
Department of Molecular Oncology, Roche Institute of Molecular Biology, Nutley, New Jersey 07110.
Skalka A M
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29 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.62.2.528-533.1988
Published
1988-02-00
Pages
528-33
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC250564
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.62.2.528-533.1988 PMC Full Text View journal details
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