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PMID: 2447483 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Tyrosine phosphorylations in vivo associated with v-fms transformation.

Molecular and cellular biology ·Vol. 8 ·No. 1 ·1988-01-00 ·Pages 176-85

Morrison DK, Browning PJ, White MF, Roberts TM

Abstract

The role of tyrosine-specific phosphorylation in v-fms-mediated transformation was examined by immunoblotting techniques together with a high-affinity antibody that is specific for phosphotyrosine. This antiphosphotyrosine antibody detected phosphorylated tyrosine residues on the gp140v-fms molecule, but not gP180v-fms or gp120v-fms, in v-fms-transformed cells. This antibody also identified a number of cellular proteins that were either newly phosphorylated on tyrosine residues or showed enhanced phosphorylation on tyrosine residues as a result of v-fms transformation. However, the substrates of the v-fms-induced tyrosine kinase activity were not the characterized pp60v-src substrates. The phosphorylation of some of these cellular proteins and of the gp140fms molecule was found to correlate with the ability of v-fms/c-fms hybrids to transform cells. In addition, immunoblotting with the phosphotyrosine antibody allowed a comparison to be made of the substrates phosphorylated on tyrosine residues in various transformed cell lines. This study indicates that the pattern of tyrosine phosphorylation in v-fms-transformed cells is strikingly similar to that in v-sis-transformed cells.

MeSH Terms
Animals Cell Line Cell Transformation, Neoplastic/physiopathology Glycoproteins/metabolism Immunologic Techniques Mice Mink Oncogene Protein pp60(v-src) Oncogene Proteins, Viral/metabolism Oncogenes Phosphorylation Phosphotyrosine Protein-Tyrosine Kinases/metabolism Proto-Oncogene Proteins/metabolism Recombinant Fusion Proteins/metabolism Retroviridae Proteins/metabolism Structure-Activity Relationship Tyrosine/analogs & derivatives,metabolism
Chemicals
Glycoproteins Oncogene Proteins, Viral Proto-Oncogene Proteins Recombinant Fusion Proteins Retroviridae Proteins Phosphotyrosine Tyrosine Protein-Tyrosine Kinases Oncogene Protein pp60(v-src)
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Morrison D K
Dana-Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115.
Browning P J
White M F
Roberts T M
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40 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.8.1.176-185.1988
Published
1988-01-00
Pages
176-85
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC363099
Subset
IM
Grants
NCI NIH HHS · CA-30002 · United States
NCI NIH HHS · CA-43803 · United States
External Links
PubMed source DOI: 10.1128/mcb.8.1.176-185.1988 PMC Full Text View journal details
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