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PMID: 6582485 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Nucleotide sequence of the feline retroviral oncogene v-fms shows unexpected homology with oncogenes encoding tyrosine-specific protein kinases.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 81 ·No. 1 ·1984-01-00 ·Pages 85-9

Hampe A, Gobet M, Sherr CJ, Galibert F

Abstract

The nucleotide sequence encoding the transforming polyprotein of the McDonough strain of feline sarcoma virus was determined. This sequence includes 231 nucleotides specifying a leader peptide, 1,377 nucleotides encoding most of the feline leukemia virus-derived gag gene, and 2,969 nucleotides representing the viral transforming gene v-fms. A single open reading frame was predicted to encode a fusion polyprotein of 160,000 daltons (P160gag-fms). Fourteen potential sites for glycosylation were predicted within the v-fms-encoded portion of the protein, consistent with previous observations that the primary translation product is rapidly glycosylated. The presence of hydrophobic signal peptides within the amino-terminal leader sequence and in the middle of the v-fms-encoded moiety suggests that the transforming glycoprotein becomes oriented with its amino terminus within the lumen of the rough endoplasmic reticulum and its carboxyl terminus protruding across the membrane of the rough endoplasmic reticulum into the cytoplasm. The latter portion of the protein shows unexpected homology to tyrosine-specific protein kinases encoded by several of the known retroviral oncogenes.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cats Cells, Cultured Cloning, Molecular Genes, Viral Mice Molecular Weight Oncogenes Protein Kinases/genetics Protein-Tyrosine Kinases Retroviridae/enzymology,genetics Sarcoma/microbiology Transfection
Chemicals
Protein Kinases Protein-Tyrosine Kinases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Hampe A
Gobet M
Sherr C J
Galibert F
References (48)
48 references, click to expand
  1. Cancer genes come of age.
    Cell. 1983 Apr;32(4):1018-20 PMID: 6340833
  2. Mutant feline sarcoma proviruses containing the viral oncogene (v-fes) and either feline or murine control elements.
    J Virol. 1983 Mar;45(3):1004-16 PMID: 6300443
  3. The Parodi-Irgens feline sarcoma virus and simian sarcoma virus have homologous oncogenes, but in different contexts of the viral genomes.
    J Virol. 1983 May;46(2):606-13 PMID: 6302323
  4. Complete sequence of the Rous sarcoma virus env gene: identification of structural and functional regions of its product.
    J Virol. 1983 Jun;46(3):920-36 PMID: 6304351
  5. Local mutagenesis of Rous sarcoma virus: the major sites of tyrosine and serine phosphorylation of pp60src are dispensable for transformation.
    Cell. 1983 Sep;34(2):597-607 PMID: 6311433
  6. The erbB gene of avian erythroblastosis virus is a member of the src gene family.
    Cell. 1983 Nov;35(1):71-8 PMID: 6313229
  7. The transforming protein of Moloney murine sarcoma virus is a soluble cytoplasmic protein.
    Cell. 1983 May;33(1):161-72 PMID: 6380747
  8. Transmissible feline fibrosarcoma.
    Nature. 1969 Mar 15;221(5185):1074-5 PMID: 5774407
  9. Site-directed point mutation in the src gene oF rous sarcoma virus results in an inactive src gene product.
    J Virol. 1983 Mar;45(3):1211-6 PMID: 6300458
  10. Analysis of the primary translational product and integrated DNA of a new feline sarcoma virus, GR-FeSV.
    Virology. 1983 Mar;125(2):502-7 PMID: 6601328
  11. Experimental transmission of feline fibrosarcoma to cats and dogs.
    Nature. 1970 May 30;226(5248):807-9 PMID: 5444620
  12. A transmissible feline fibrosarcoma of viral origin.
    Cancer Res. 1971 Jul;31(7):953-6 PMID: 4997847
  13. A possible precursor of immunoglobulin light chains.
    Nat New Biol. 1972 Sep 27;239(91):117-20 PMID: 4507519
  14. Tunicamycin--an inhibitor of yeast glycoprotein synthesis.
    Biochem Biophys Res Commun. 1974 May 7;58(1):287-95 PMID: 4598444
  15. Membrane proteins: amino acid sequence and membrane penetration.
    J Mol Biol. 1974 Aug 25;87(4):853-8 PMID: 4427380
  16. Tunicamycin inhibition of polyisoprenyl N-acetylglucosaminyl pyrophosphate formation in calf-liver microsomes.
    Biochem Biophys Res Commun. 1975 Jul 8;65(1):248-57 PMID: 167767
  17. Transfer of proteins across membranes. I. Presence of proteolytically processed and unprocessed nascent immunoglobulin light chains on membrane-bound ribosomes of murine myeloma.
    J Cell Biol. 1975 Dec;67(3):835-51 PMID: 811671
  18. Feline leukemia virus: biochemical and immunological characterization of gag gene-coded structural proteins.
    J Virol. 1977 Sep;23(3):599-607 PMID: 197262
  19. Glycosylation of a membrane protein is restricted to the growing polypeptide chain but is not necessary for insertion as a transmembrane protein.
    Cell. 1978 Dec;15(4):1447-54 PMID: 215329
  20. Amino-terminal sequence of bovine leukemia virus major internal protein: homology with mammalian type C virus p30 structural proteins.
    Proc Natl Acad Sci U S A. 1979 Jun;76(6):2996-3000 PMID: 223166
  21. Nature and distribution of feline sarcoma virus nucleotide sequences.
    J Virol. 1979 Jun;30(3):821-7 PMID: 225544
  22. The nonstructural components of polyproteins encoded by replication-defective mammalian transforming retroviruses are phosphorylated and have associated protein kinase activity.
    Virology. 1980 Feb;101(1):185-97 PMID: 6243819
  23. Biochemical and immunological characterization of polyproteins coded for by the McDonough, Gardner-Arnstein, and Snyder-Theilen strains of feline sarcoma virus.
    J Virol. 1980 Jan;33(1):196-207 PMID: 6154148
  24. Molecular cloning of Snyder-Theilen feline leukemia and sarcoma viruses: comparative studies of feline sarcoma virus with its natural helper virus and with Moloney murine sarcoma virus.
    J Virol. 1980 Apr;34(1):200-12 PMID: 6246254
  25. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  26. Intracellular protein topogenesis.
    Proc Natl Acad Sci U S A. 1980 Mar;77(3):1496-500 PMID: 6929499
  27. Characterization of a 170,000-dalton polyprotein encoded by the McDonough strain of feline sarcoma virus.
    J Virol. 1980 Jul;35(1):165-75 PMID: 6251265
  28. Three independent isolates of feline sarcoma virus code for three distinct gag-x polyproteins.
    J Virol. 1980 Jul;35(1):259-64 PMID: 6251274
  29. Origin and functional properties of the major gene product of the Snyder-Theilen strain of feline sarcoma virus.
    Proc Natl Acad Sci U S A. 1980 Sep;77(9):5158-62 PMID: 6254060
  30. Nucleotide sequence and formation of the transforming gene of a mouse sarcoma virus.
    Nature. 1981 Jan 22;289(5795):258-62 PMID: 6256659
  31. Homology exists among the transforming sequences of avian and feline sarcoma viruses.
    Proc Natl Acad Sci U S A. 1980 Nov;77(11):6536-40 PMID: 6256742
  32. Structural and functional domains of the Rous sarcoma virus transforming protein (pp60src).
    Proc Natl Acad Sci U S A. 1981 Mar;78(3):1624-8 PMID: 6262821
  33. Structure of glycosylated and unglycosylated gag polyproteins of Rauscher murine leukemia virus: carbohydrate attachment sites.
    J Virol. 1981 May;38(2):581-92 PMID: 7241663
  34. Recombinant bacteriophages containing the integrated transforming provirus of Gardner--Arnstein feline sarcoma virus.
    Proc Natl Acad Sci U S A. 1981 Jul;78(7):4036-40 PMID: 6270655
  35. Gene products of McDonough feline sarcoma virus have an in vitro-associated protein kinase that phosphorylates tyrosine residues: lack of detection of this enzymatic activity in vivo.
    J Virol. 1981 Dec;40(3):812-21 PMID: 6275118
  36. Proposal for naming host cell-derived inserts in retrovirus genomes.
    J Virol. 1981 Dec;40(3):953-7 PMID: 7321107
  37. Origin and biological properties of a new feline sarcoma virus.
    Virology. 1982 Feb;117(1):238-44 PMID: 6175084
  38. Analysis of the sequence of amino acids surrounding sites of tyrosine phosphorylation.
    Proc Natl Acad Sci U S A. 1982 Feb;79(4):973-7 PMID: 6280176
  39. McDonough feline sarcoma virus: characterization of the molecularly cloned provirus and its feline oncogene (v-fms).
    J Virol. 1982 Feb;41(2):489-500 PMID: 6281462
  40. Viral src gene products are related to the catalytic chain of mammalian cAMP-dependent protein kinase.
    Proc Natl Acad Sci U S A. 1982 May;79(9):2836-9 PMID: 6283546
  41. Nucleotide sequences of feline retroviral oncogenes (v-fes) provide evidence for a family of tyrosine-specific protein kinase genes.
    Cell. 1982 Oct;30(3):775-85 PMID: 6183005
  42. Nucleotide sequence of Fujinami sarcoma virus: evolutionary relationship of its transforming gene with transforming genes of other sarcoma viruses.
    Cell. 1982 Oct;30(3):787-95 PMID: 6291784
  43. Monoclonal antibodies to the transformation-specific glycoprotein encoded by the feline retroviral oncogene v-fms.
    J Virol. 1982 Nov;44(2):696-702 PMID: 6292527
  44. Nucleotide sequences of feline sarcoma virus long terminal repeats and 5' leaders show extensive homology to those of other mammalian retroviruses.
    J Virol. 1983 Jan;45(1):466-72 PMID: 6296453
  45. Identification and characterization of the avian erythroblastosis virus erbB gene product as a membrane glycoprotein.
    Cell. 1983 Feb;32(2):579-88 PMID: 6297801
  46. Antibodies of predetermined specificity detect two retroviral oncogene products and inhibit their kinase activities.
    Proc Natl Acad Sci U S A. 1983 Mar;80(5):1246-50 PMID: 6572383
  47. Phosphorylation of tyrosine-416 is not required for the transforming properties and kinase activity of pp60v-src.
    Cell. 1983 Mar;32(3):891-901 PMID: 6403250
  48. The product of the avian erythroblastosis virus erbB locus is a glycoprotein.
    Cell. 1983 Apr;32(4):1257-67 PMID: 6301688
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.81.1.85
Published
1984-01-00
Pages
85-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC344615
Subset
IM
Databases
GENBANK
K01643
External Links
PubMed source DOI: 10.1073/pnas.81.1.85 PMC Full Text View journal details
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