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PMID: 2447829 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Isolation and characterization of Streptococcus cremoris Wg2-specific promoters.

Applied and environmental microbiology ·Vol. 53 ·No. 10 ·1987-10-00 ·Pages 2452-7

van der Vossen JM, van der Lelie D, Venema G

Abstract

By cloning MboI fragments in the promoter selection vector pGKV210, which replicates in Streptococcus lactis, Bacillus subtilis, and Escherichia coli and carries a promoterless chloramphenicol acetyltransferase gene, we obtained a number of fragments endowed with promoter activity, partly by direct selection for chloramphenicol resistance in S. lactis IL1403 and partly by selection in B. subtilis. Five fragments were sequenced, and the promoters were mapped with S1 nuclease. The promoters agreed with the E. coli promoter consensus and the B. subtilis vegetative sigma 43 promoter consensus. The promoters were preceded by an A + T-rich region (ranging from 64 to 78% A + T). S1 nuclease mapping data showed that the transcriptional start point in three of the fragments was at a TAG sequence 5 to 9 nucleotides downstream from the promoter. Three fragments carried an open reading frame preceded by a ribosome-binding site which can be recognized by E. coli, B. subtilis, and S. lactis ribosomes.

MeSH Terms
Bacillus subtilis/genetics Base Sequence Cloning, Molecular DNA, Bacterial/genetics Escherichia coli/genetics Genes, Bacterial Lactococcus lactis/genetics Molecular Sequence Data Nucleotide Mapping Promoter Regions, Genetic RNA, Bacterial/genetics Streptococcus/genetics Transcription, Genetic beta-Galactosidase/genetics
Chemicals
DNA, Bacterial RNA, Bacterial beta-Galactosidase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
van der Vossen J M
Department of Genetics, University of Groningen, Haren, The Netherlands.
van der Lelie D
Venema G
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Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
DOI
10.1128/aem.53.10.2452-2457.1987
Published
1987-10-00
Pages
2452-7
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC204128
Subset
IM
External Links
PubMed source DOI: 10.1128/aem.53.10.2452-2457.1987 PMC Full Text View journal details
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