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PMID: 2448242 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Cross-neutralization of staphylococcal and streptococcal pyrogenic toxins by monoclonal and polyclonal antibodies.

Infection and immunity ·Vol. 56 ·No. 2 ·1988-02-00 ·Pages 400-4

Bohach GA, Hovde CJ, Handley JP, Schlievert PM

Abstract

We evaluated cross-reactivity of antibodies against staphylococcal and streptococcal pyrogenic toxins. Monoclonal antibodies against staphylococcal enterotoxin (ET) C1 and streptococcal pyrogenic exotoxin (SPE) A were tested for reactivity with homologous and heterologous pyrogenic toxins in vitro. Ten immunoglobulin G1 anti-ET C1 monoclonal antibodies showed little or no cross-reactivity in an enzyme-linked immunosorbent assay, but many of these could neutralize the mitogenic effect of ET B, SPE A, or both. Two immunoglobulin M anti-ET C1 monoclonal antibodies and eight immunoglobulin M anti-SPE A monoclonal antibodies showed extensive cross-reactivity in the enzyme-linked immunosorbent assay and the mitogenicity neutralization assay. No cross-reactivity was observed with SPE C or toxic shock syndrome toxin 1. Rabbits immunized against ET B, ET C1, or SPE A were resistant to challenge with the immunizing toxin. In addition, reciprocal immunity was stimulated by the two ETs, and immunity to SPE A provided protection against ET B but not ET C1. These results show that staphylococcal and streptococcal pyrogenic toxins which share sequence homology have common antigenic determinants which may not be detected in Ouchterlony immunodiffusion assays.

MeSH Terms
Animals Antibodies, Monoclonal/immunology Bacterial Toxins/immunology Epitopes Lymphocyte Activation/drug effects Mice Neutralization Tests Pyrogens/immunology Rabbits Staphylococcus/immunology Staphylococcus aureus/immunology
Chemicals
Antibodies, Monoclonal Bacterial Toxins Epitopes Pyrogens
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Bohach G A
Department of Microbiology, University of Minnesota Medical School, Minneapolis 55455.
Hovde C J
Handley J P
Schlievert P M
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29 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.56.2.400-404.1988
Published
1988-02-00
Pages
400-4
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC259295
Subset
IM
Grants
NHLBI NIH HHS · HL36611 · United States
External Links
PubMed source DOI: 10.1128/iai.56.2.400-404.1988 PMC Full Text View journal details
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