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PMID: 2448293 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Expression of an Agrobacterium Ti plasmid gene involved in cytokinin biosynthesis is regulated by virulence loci and induced by plant phenolic compounds.

Journal of bacteriology ·Vol. 170 ·No. 2 ·1988-02-00 ·Pages 790-5

John MC, Amasino RM

Abstract

The nopaline-type Ti plasmid T37 of Agrobacterium tumefaciens carries two distinct genes that encode enzymes involved in cytokinin biosynthesis. In this report, we show that the level of expression of one of these genes was increased dramatically by culture conditions that increased the expression of Ti plasmid virulence genes, including coculture with plant cells or treatment with acetosyringone, a plant phenolic compound. When this nopaline-type Ti plasmid gene was introduced into Agrobacterium strains containing an octopine-type Ti plasmid, similar induction of expression by culture conditions was observed, and analysis of virulence region mutants demonstrated that this induction was under the control of the virA and virG regulatory loci. We further show that induction was strongly pH dependent in octopine strains but, under the conditions examined, pH independent in nopaline strains.

MeSH Terms
Acetophenones/pharmacology Autoradiography Cytokinins/biosynthesis Gene Expression Regulation Genes, Bacterial Genes, Regulator Hydrogen-Ion Concentration Nucleic Acid Hybridization Plant Growth Regulators/biosynthesis Plant Tumors Plasmids RNA, Bacterial/genetics RNA, Messenger/genetics Rhizobium/genetics,metabolism Sequence Homology, Nucleic Acid
Chemicals
Acetophenones Cytokinins Plant Growth Regulators RNA, Bacterial RNA, Messenger acetosyringone
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
John M C
Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin-Madison 53706-1569.
Amasino R M
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25 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.170.2.790-795.1988
Published
1988-02-00
Pages
790-5
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC210723
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.170.2.790-795.1988 PMC Full Text View journal details
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