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PMID: 2451126 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Dictyostelium discoideum myosin: isolation and characterization of cDNAs encoding the essential light chain.

Molecular and cellular biology ·Vol. 8 ·No. 2 ·1988-02-00 ·Pages 794-801

Chisholm RL, Rushforth AM, Pollenz RS, Kuczmarski ER, Tafuri SR

Abstract

We used an antibody specific for Dictyostelium discoideum myosin to screen a lambda gt11 cDNA expression library to obtain cDNA clones which encode the Dictyostelium essential myosin light chain (EMLC). The amino acid sequence predicted from the sequence of the cDNA clone showed 31.5% identity with the amino acid sequence of the chicken EMLC. Comparisons of the Dictyostelium EMLC, a nonmuscle cell type, with EMLC sequences from similar MLCs of skeletal- and smooth-muscle origin, showed distinct regions of homology. Much of the observed homology was localized to regions corresponding to consensus Ca2+-binding of E-F hand domains. Southern blot analysis suggested that the Dictyostelium genome contains a single gene encoding the EMLC. Examination of the pattern of EMLC mRNA expression showed that a significant increase in EMLC message levels occurred during the first few hours of development, coinciding with increased actin expression and immediately preceding the period of maximal chemotactic activity.

MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular DNA, Fungal/genetics,isolation & purification Dictyostelium/genetics Epitopes/analysis Genes Genes, Fungal Molecular Sequence Data Myosin Subfragments Myosins/genetics Nucleic Acid Hybridization Peptide Fragments/genetics
Chemicals
DNA, Fungal Epitopes Myosin Subfragments Peptide Fragments Myosins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Chisholm R L
Department of Cell Biology and Anatomy, Northwestern University Medical School, Chicago, Illinois 60611.
Rushforth A M
Pollenz R S
Kuczmarski E R
Tafuri S R
References (55)
55 references, click to expand
  1. Structural and functional changes of myosin during development: comparison with adult fast, slow and cardiac myosin.
    Dev Biol. 1975 Oct;46(2):317-25 PMID: 241672
  2. Regulation and kinetics of the actin-myosin-ATP interaction.
    Annu Rev Biochem. 1980;49:921-56 PMID: 6447472
  3. Nonmuscle contractile proteins: the role of actin and myosin in cell motility and shape determination.
    Annu Rev Biochem. 1977;46:797-822 PMID: 143236
  4. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  5. Cytoplasmic structure and contractility in amoeboid cells.
    Int Rev Cytol. 1979;56:57-144 PMID: 37189
  6. Distribution of myosin isoenzymes among skeletal muscle fiber types.
    J Cell Biol. 1979 Apr;81(1):10-25 PMID: 90047
  7. The contractile basis of ameboid movement. VI. The solation-contraction coupling hypothesis.
    J Cell Biol. 1979 Dec;83(3):633-48 PMID: 42649
  8. Structure and evolution of calcium-modulated proteins.
    CRC Crit Rev Biochem. 1980;8(2):119-74 PMID: 6105043
  9. Localization of g2 light chain in the link between the heads and tail of cardiac myosin.
    J Biochem. 1980 Jun;87(6):1603-7 PMID: 7400112
  10. Complexity of nuclear and polysomal RNAs in growing Dictyostelium discoideum cells.
    Dev Biol. 1980 Aug;78(2):268-84 PMID: 6157589
  11. Actin activation of Ca2+-sensitive Mg2+-ATPase activity of Acanthamoeba myosin II is enhanced by dephosphorylation of its heavy chains.
    J Biol Chem. 1980 Sep 10;255(17):8011-4 PMID: 6106017
  12. Cytoplasmic processing of myosin heavy chain messenger RNA: evidence provided by using a recombinant DNA plasmid.
    Proc Natl Acad Sci U S A. 1980 Oct;77(10):5749-53 PMID: 6934508
  13. Regulation of myosin self-assembly: phosphorylation of Dictyostelium heavy chain inhibits formation of thick filaments.
    Proc Natl Acad Sci U S A. 1980 Dec;77(12):7292-6 PMID: 6452632
  14. Differential expression and 5' end mapping of actin genes in Dictyostelium.
    Cell. 1981 Jun;24(3):799-807 PMID: 6894715
  15. Hydrolysis of ATP and reversible binding to F-actin by myosin heavy chains free of all light chains.
    Nature. 1981 Aug 6;292(5823):560-2 PMID: 6454850
  16. Proximity of alkali light chains to 27K domain of the heavy chain in myosin subfragment 1.
    Biochem Biophys Res Commun. 1981 Sep 16;102(1):466-75 PMID: 6458297
  17. Amino acid sequences of the cardiac L-2A, L-2B and gizzard 17 000-Mr light chains of chicken muscle myosin.
    FEBS Lett. 1981 Dec 7;135(2):232-6 PMID: 7319048
  18. The free heavy chain of vertebrate skeletal myosin subfragment 1 shows full enzymatic activity.
    J Biol Chem. 1982 Jan 25;257(2):1102-5 PMID: 6119311
  19. Chemoresponsiveness to cAMP and folic acid during growth, development, and dedifferentiation in Dictyostelium discoideum.
    Differentiation. 1981;18(3):151-60 PMID: 6276256
  20. Distribution and properties of myosin isozymes in developing avian and mammalian skeletal muscle fibers.
    J Cell Biol. 1982 Feb;92(2):471-84 PMID: 6174531
  21. A 40,000-dalton protein from Dictyostelium discoideum affects assembly properties of actin in a Ca2+-dependent manner.
    J Cell Biol. 1982 Apr;93(1):205-10 PMID: 7068756
  22. Cloning and characterization of cDNA sequences corresponding to myosin light chains 1, 2, and 3, troponin-C, troponin-T, alpha-tropomyosin, and alpha-actin.
    J Biol Chem. 1982 Sep 25;257(18):11078-86 PMID: 6179945
  23. Crosslinking by thiol disulfide interchange of 5,5'-dithiobis(2-nitrobenzoic acid)-treated light chain and heavy chain of rabbit skeletal myosin.
    Eur J Biochem. 1982 Sep 1;126(3):603-9 PMID: 6754373
  24. The binding of smooth muscle heavy meromyosin to actin in the presence of ATP. Effect of phosphorylation.
    J Biol Chem. 1982 Dec 10;257(23):13880-3 PMID: 6128340
  25. Mechanism of interaction of Dictyostelium severin with actin filaments.
    J Cell Biol. 1982 Dec;95(3):711-9 PMID: 6897549
  26. Sequence organization in Dictyostelium: unique structure at the 5'-ends of protein coding genes.
    Nucleic Acids Res. 1983 Jan 25;11(2):541-52 PMID: 6828377
  27. Efficient isolation of genes by using antibody probes.
    Proc Natl Acad Sci U S A. 1983 Mar;80(5):1194-8 PMID: 6219389
  28. Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination.
    Proc Natl Acad Sci U S A. 1983 Jul;80(13):3963-5 PMID: 6575390
  29. On the mode of the alkali light chain association to the heavy chain of myosin subfragment 1. Evidence for the involvement of the carboxyl-terminal region of the heavy chain.
    Biochemistry. 1983 Jun 21;22(13):3046-53 PMID: 6882736
  30. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
    Anal Biochem. 1983 Jul 1;132(1):6-13 PMID: 6312838
  31. Electron microscopy of scallop myosin. Location of regulatory light chains.
    J Mol Biol. 1983 Sep 25;169(3):723-41 PMID: 6415287
  32. A comprehensive set of sequence analysis programs for the VAX.
    Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387-95 PMID: 6546423
  33. A convenient and adaptable package of computer programs for DNA and protein sequence management, analysis and homology determination.
    Nucleic Acids Res. 1984 Jan 11;12(1 Pt 2):643-55 PMID: 6320100
  34. Alternative transcription and two modes of splicing results in two myosin light chains from one gene.
    Nature. 1984 Mar 22-28;308(5957):333-8 PMID: 6709041
  35. Drosophila melanogaster has only one myosin alkali light-chain gene which encodes a protein with considerable amino acid sequence homology to chicken myosin alkali light chains.
    Mol Cell Biol. 1984 May;4(5):956-65 PMID: 6328279
  36. Mechanism of sequential induction of cell-type specific mRNAs in Dictyostelium differentiation.
    Nature. 1984 Jul 5-11;310(5972):67-9 PMID: 6429548
  37. Myosin.
    Annu Rev Biochem. 1984;53:35-73 PMID: 6383197
  38. A single locus in the mouse encodes both myosin light chains 1 and 3, a second locus corresponds to a related pseudogene.
    Cell. 1984 Nov;39(1):129-40 PMID: 6091905
  39. Fast skeletal muscle myosin light chains 1 and 3 are produced from a single gene by a combined process of differential RNA transcription and splicing.
    J Biol Chem. 1984 Nov 10;259(21):13595-604 PMID: 6092382
  40. Developmental variations in the splicing pattern of transcripts from the Drosophila gene encoding myosin alkali light chain result in different carboxyl-terminal amino acid sequences.
    Proc Natl Acad Sci U S A. 1985 Jan;82(2):449-53 PMID: 2982157
  41. DNA-mediated transformation in Dictyostelium discoideum: regulated expression of an actin gene fusion.
    Mol Cell Biol. 1984 Dec;4(12):2890-8 PMID: 6098825
  42. Identification of human glucocorticoid receptor complementary DNA clones by epitope selection.
    Science. 1985 May 10;228(4700):740-2 PMID: 2581314
  43. Cloning and characterization of a nonmuscle myosin heavy chain cDNA.
    Proc Natl Acad Sci U S A. 1985 Oct;82(20):6807-10 PMID: 3901008
  44. Rapid transfer of DNA from agarose gels to nylon membranes.
    Nucleic Acids Res. 1985 Oct 25;13(20):7207-21 PMID: 4059056
  45. Chemoattractant-elicited increases in myosin phosphorylation in Dictyostelium.
    Cell. 1985 Nov;43(1):307-14 PMID: 3000604
  46. Phenocopy of discoidin I-minus mutants by antisense transformation in Dictyostelium.
    Cell. 1985 Dec;43(3 Pt 2):633-41 PMID: 4075402
  47. Myosin light-chain 1 and 3 gene has two structurally distinct and differentially regulated promoters evolving at different rates.
    Mol Cell Biol. 1985 Nov;5(11):3168-82 PMID: 3018505
  48. The essential light chains constitute part of the active site of smooth muscle myosin.
    Nature. 1986 Nov 6-12;324(6092):78-80 PMID: 3641060
  49. Conserved protein domains in a myosin heavy chain gene from Dictyostelium discoideum.
    Proc Natl Acad Sci U S A. 1986 Dec;83(24):9433-7 PMID: 3540939
  50. Partial purification of two myosin heavy chain kinases from Dictyostelium discoideum.
    J Muscle Res Cell Motil. 1986 Dec;7(6):501-9 PMID: 3027120
  51. Myosin specific phosphatases isolated from Dictyostelium discoideum.
    J Muscle Res Cell Motil. 1986 Dec;7(6):510-6 PMID: 3027121
  52. Myosin light chain kinase and myosin light chain phosphatase from Dictyostelium: effects of reversible phosphorylation on myosin structure and function.
    J Cell Biol. 1987 May;104(5):1309-23 PMID: 3032987
  53. Antisense RNA inactivation of myosin heavy chain gene expression in Dictyostelium discoideum.
    Science. 1987 May 29;236(4805):1081-6 PMID: 3576221
  54. Disruption of the Dictyostelium myosin heavy chain gene by homologous recombination.
    Science. 1987 May 29;236(4805):1086-91 PMID: 3576222
  55. Purification and structural analysis of myosins from brain and other non-muscle tissues.
    J Mol Biol. 1975 Nov 25;99(1):1-14 PMID: 128633
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.8.2.794-801.1988
Published
1988-02-00
Pages
794-801
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC363206
Subset
IM
Databases
GENBANK
M17923, M19337
External Links
PubMed source DOI: 10.1128/mcb.8.2.794-801.1988 PMC Full Text View journal details
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