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PMID: 2453884 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Use of an Epstein-Barr virus episomal replicon for anti-sense RNA-mediated gene inhibition in a human cytotoxic T-cell clone.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 11 ·1988-06-00 ·Pages 4010-4

Hambor JE, Hauer CA, Shu HK, Groger RK, Kaplan DR, Tykocinski ML

Abstract

A methodology was developed for stable gene transfer into cloned nontransformed human T lymphocytes. Stable high-level gene expression was achieved in cloned human T cells by using a self-replicating Epstein-Barr virus (EBV) episomal replicon. A comparison of five eukaryotic promoters established that the Rous sarcoma virus 3' long terminal repeat (RSV 3' LTR) and the lymphopapilloma virus (LPV) 5' LTR are optimal for episome-based expression in T cells. Effective (greater than 95%), selective, and reversible anti-sense RNA-mediated gene inhibition of a model T-cell-associated molecule (CD8) was achieved in a cytotoxic human T-cell clone by using an EBV episome-based, RSV 3' LTR-driven expression system. The linking of anti-sense RNA mutagenesis and T-cell cloning technologies should contribute significantly to studies of human T-cell function.

MeSH Terms
Antigens, Differentiation, T-Lymphocyte/genetics CD8 Antigens Cloning, Molecular Gene Expression Regulation Genetic Vectors Herpesvirus 4, Human/genetics Plasmids RNA/genetics RNA, Complementary Replicon T-Lymphocytes, Cytotoxic/physiology Transfection
Chemicals
Antigens, Differentiation, T-Lymphocyte CD8 Antigens RNA, Complementary RNA
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Hambor J E
Institute of Pathology, Case Western Reserve University, Cleveland, OH 44106.
Hauer C A
Shu H K
Groger R K
Kaplan D R
Tykocinski M L
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.85.11.4010
Published
1988-06-00
Pages
4010-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC280350
Subset
IM
Grants
NCI NIH HHS · P30 CA 43703 · United States
NIAID NIH HHS · R01 AI 22505 · United States
NCI NIH HHS · R01 CA 36189 · United States
External Links
PubMed source DOI: 10.1073/pnas.85.11.4010 PMC Full Text View journal details
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