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PMID: 2459713 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Two monoclonal rat antibodies with specificity for the beta-chain variable region V beta 6 of the murine T-cell receptor.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 20 ·1988-10-00 ·Pages 7695-8

Payne J, Huber BT, Cannon NA, Schneider R, Schilham MW, Acha-Orbea H, MacDonald HR, Hengartner H

Abstract

Two rat monoclonal antibodies (mAbs), 44-22-1 and 46-6B5, which recognize an alloreactive cytotoxic clone, 3F9, have been further tested on a panel of T hybridomas and cytotoxic T-cell clones for binding and functional activities. The mAbs recognized only those cells sharing the expression of the T-cell receptor beta-chain variable region gene V beta 6 with 3F9. All V beta 6+ cells were activated by these mAbs under cross-linking conditions and their antigen-specific activation was blocked by soluble mAb. Furthermore, depletion of 46-6B5+ normal lymph node T cells eliminated all cells expressing the epitope recognized by 44-22-1 and V beta 6 mRNA.

MeSH Terms

Animals Antibodies, Monoclonal/immunology Antibody Specificity Cell Line Clone Cells Cricetinae Epitopes/immunology Hybridomas Mice Rats Receptors, Antigen, T-Cell/immunology T-Lymphocytes/immunology T-Lymphocytes, Cytotoxic/immunology T-Lymphocytes, Helper-Inducer/immunology

Chemicals

Antibodies, Monoclonal Epitopes Receptors, Antigen, T-Cell

Authors & Affiliations

8 authors, click to expand affiliations / ORCID

Payne J

Department of Pathology, Tufts University School of Medicine, Boston, MA 02111.

Huber B T

Cannon N A

Schneider R

Schilham M W

Acha-Orbea H

MacDonald H R

Hengartner H

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20 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.85.20.7695

Published

1988-10-00

Pages

7695-8

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC282259

Subset

Grants

NIAID NIH HHS · AI14910 · United States

NIAID NIH HHS · AI23031 · United States

NIAID NIH HHS · AI23495 · United States

External Links

PubMed source DOI: 10.1073/pnas.85.20.7695 PMC Full Text View journal details

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Two monoclonal rat antibodies with specificity for the beta-chain variable region V beta 6 of the murine T-cell receptor.

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