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PMID: 2461092 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The effects of estrogens on tumor growth and on prolactin and growth hormone mRNA expression in rat pituitary tissues.

The American journal of pathology ·Vol. 133 ·No. 2 ·1988-11-00 ·Pages 397-406

Lloyd RV, Cano M, Landefeld TD

Abstract

Estrogens inhibit tumor growth and modify PRL and GH expression in the MtT/W15 transplantable rat pituitary tumor. The effects of estradiol (E2) and diethylstilbestrol (DES) on PRL and GH mRNA levels were investigated. Estrogens increased GH mRNA levels and decreased PRL mRNA levels as detected by in situ hybridization and Northern blot hybridization with oligonucleotide probes, while inhibiting tumor growth. Similar changes in immunoreactive GH and PRL were seen in the tumor cells. The pituitary glands of tumor-bearing rats treated with estrogen for 3 weeks were increased in weight with a concurrent increase in pituitary PRL mRNA when analyzed by dot blot hybridization. These results indicate that estrogens have an inhibitory effect on the growth of the MtT/W15 tumor and increase GH protein and mRNA levels, while causing PRL protein and mRNA levels to decrease. The pituitaries of tumor-bearing rats concomitantly undergo PRL cell hyperplasia with an increase in PRL mRNA. These results also demonstrate a paradoxical effect of estrogens on different pituitary tissues.

MeSH Terms
Animals Cell Division/drug effects Diethylstilbestrol/pharmacology Estradiol/pharmacology Estrogens/pharmacology Female Growth Hormone/genetics Immunoblotting Nucleic Acid Hybridization Pituitary Gland/analysis Pituitary Neoplasms/pathology Prolactin/genetics RNA RNA, Messenger/analysis Rats Rats, Inbred WF
Chemicals
Estrogens RNA, Messenger Estradiol RNA Diethylstilbestrol Prolactin Growth Hormone
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Lloyd R V
Department of Pathology, University of Michigan Medical School, Ann Arbor.
Cano M
Landefeld T D
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Article Info
Journal
The American journal of pathology
Abbr.
Am J Pathol
ISSN
0002-9440
Published
1988-11-00
Pages
397-406
Language
English
Region
United States
NLM ID
0370502
PMCID
PMC1880788
Subset
IM
Grants
NCI NIH HHS · CA37238 · United States
NCI NIH HHS · CA42951 · United States
NICHD NIH HHS · HD12016 · United States
External Links
PubMed source PMC Full Text View journal details
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