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PMID: 2461962 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

In vitro proliferative responses and antibody titers specific to human acetylcholine receptor synthetic peptides in patients with myasthenia gravis and relation to HLA class II genes.

The Journal of clinical investigation ·Vol. 82 ·No. 6 ·1988-12-00 ·Pages 1894-900

Brocke S, Brautbar C, Steinman L, Abramsky O, Rothbard J, Neumann D, Fuchs S, Mozes E

Abstract

To investigate which parts of the acetylcholine receptor are involved in the initiation and development of myasthenia gravis (MG), peptides representing different sequences of the human acetylcholine receptor alpha-subunit were synthesized. These peptides were tested for their ability to stimulate T cells of myasthenic patients and healthy control patients in proliferation assays and to bind to sera antibodies. Three of eight peptides discriminated significantly between the two groups in the proliferation assay, as well as in their ability to bind to serum antibodies. HLA-DR3 and DR5 were associated with proliferative responses to specific AChR peptides in the group of myasthenics. Acetylcholine receptor epitopes that might play a specific role in myasthenia gravis thus were demonstrated.

MeSH Terms
Amino Acid Sequence Cell Division/drug effects Epitopes/analysis,immunology Genes, MHC Class II HLA-D Antigens/genetics HLA-DR Antigens/genetics HLA-DR3 Antigen HLA-DR5 Antigen Humans Macromolecular Substances Molecular Sequence Data Myasthenia Gravis/immunology Receptors, Cholinergic/chemical synthesis,immunology
Chemicals
Epitopes HLA-D Antigens HLA-DR Antigens HLA-DR3 Antigen HLA-DR5 Antigen Macromolecular Substances Receptors, Cholinergic
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Brocke S
Department of Chemical Immunology, Weizmann Institute of Science, Rehovot, Israel.
Brautbar C
Steinman L
Abramsky O
Rothbard J
Neumann D
Fuchs S
Mozes E
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Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/JCI113807
Published
1988-12-00
Pages
1894-900
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC442769
Subset
IM
External Links
PubMed source DOI: 10.1172/JCI113807 PMC Full Text View journal details
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