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PMID: 2467992 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Identification of a neutralizing epitope on glycoprotein gp58 of human cytomegalovirus.

Journal of virology ·Vol. 63 ·No. 5 ·1989-05-00 ·Pages 1995-2001

Utz U, Britt W, Vugler L, Mach M

Abstract

Human cytomegalovirus contains an envelope glycoprotein of 58 kilodaltons (gp58). The protein, which is derived from a glycosylated precursor molecule of 160 kilodaltons via proteolytic cleavage, is capable of inducing neutralizing antibodies. We have mapped the epitopes recognized by the neutralizing monoclonal antibody 7-17 and a second antibody (27-287) which is not neutralizing. Overlapping fragments of the carboxy-terminal part of the open reading frame coding for gp58 were expressed in Escherichia coli as beta-galactosidase fusion proteins. The reactivities of antibodies 7-17 and 27-287 were determined by Western blot (immunoblot) analysis. Both antibodies recognized sequences between amino acids 608 and 625 of the primary gp58 translation product. The antibodies almost completely inhibited one another in a competitive binding assay with intact virus as antigen. Moreover, antibody 27-287 was able to inhibit the complement-independent neutralizing activity of antibody 7-17.

MeSH Terms
Antibodies, Monoclonal/immunology Antigens, Viral/genetics Binding, Competitive Chromosome Mapping Cytomegalovirus/genetics,immunology Epitopes Neutralization Tests Recombinant Proteins/immunology Restriction Mapping Solubility Viral Envelope Proteins/genetics,immunology
Chemicals
Antibodies, Monoclonal Antigens, Viral Epitopes Recombinant Proteins Viral Envelope Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Utz U
Institut für Klinische und Molekulare Virologie, Universität Erlangen-Nürnberg, Federal Republic of Germany.
Britt W
Vugler L
Mach M
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.63.5.1995-2001.1989
Published
1989-05-00
Pages
1995-2001
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC250614
Subset
IM
Grants
NICHD NIH HHS · HD10699 · United States
External Links
PubMed source DOI: 10.1128/JVI.63.5.1995-2001.1989 PMC Full Text View journal details
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