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PMID: 2474670 Published · ppublish English Journal Article

Generation and characterization of monoclonal antibodies to the putative CD4-binding domain of human immunodeficiency virus type 1 gp120.

Journal of virology ·Vol. 63 ·No. 9 ·1989-09-00 ·Pages 3579-85

Sun NC, Ho DD, Sun CR, Liou RS, Gordon W, Fung MS, Li XL, Ting RC, Lee TH, Chang NT

Abstract

A panel of seven monoclonal antibodies against the relatively conserved CD4-binding domain on human immunodeficiency virus type 1 (HIV-1) gp120 was generated by immunizing mice with purified gp120. These monoclonal antibodies reacted specifically with gp120 in an enzyme-linked immunosorbent assay and Western blots (immunoblots). By using synthetic peptides as antigens in the immunosorbent assay, the epitopes of these seven monoclonal antibodies were mapped to amino acid residues 423 to 437 of gp120. Further studies with radioimmunoprecipitation assays showed that they cross-reacted with both gp120 and gp160 of diverse HIV-1 isolates (HTLV-IIIB, HTLV-IIIRF, HTLV-IIIAL, and HTLV-IIIWMJ). They also bound specifically to H9 cells infected with HTLV-IIIB, HTLV-IIIRF, HTLV-IIIAL, HTLV-IIIZ84, and HTLV-IIIZ34 in indirect immunofluorescence studies. In addition, they blocked effectively the binding of HIV-1 to CD4+ C8166 cells. Despite the similarity of these properties, the monoclonal antibodies differed in neutralizing activity against HTLV-IIIB, HTLV-IIIRF, and HTLV-IIIAL, as demonstrated in both syncytium-forming assays and infectivity assays. Our findings suggest that these group-specific monoclonal antibodies to the putative CD4-binding domain on gp120 are potential candidates for development of therapeutic agents against acquired immunodeficiency disease syndrome.

MeSH Terms
Animals Antibodies, Monoclonal/biosynthesis,immunology Antigens, Differentiation, T-Lymphocyte/immunology Epitopes/analysis HIV/immunology HIV Envelope Protein gp120 Male Mice Mice, Inbred BALB C Neutralization Tests Receptors, HIV Receptors, Virus/analysis,immunology Retroviridae Proteins/immunology
Chemicals
Antibodies, Monoclonal Antigens, Differentiation, T-Lymphocyte Epitopes HIV Envelope Protein gp120 Receptors, HIV Receptors, Virus Retroviridae Proteins
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Sun N C
Tanox Biosystems, Inc., Houston, Texas 77025.
Ho D D
Sun C R
Liou R S
Gordon W
Fung M S
Li X L
Ting R C
Lee T H
Chang N T
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.63.9.3579-3585.1989
Published
1989-09-00
Pages
3579-85
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC250947
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.63.9.3579-3585.1989 PMC Full Text View journal details
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