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PMID: 2477797 Published · ppublish English Journal Article

Differential degradation of the Escherichia coli polynucleotide phosphorylase mRNA.

Nucleic acids research ·Vol. 17 ·No. 18 ·1989-09-25 ·Pages 7441-51

Takata R, Izuhara M, Hori K

Abstract

The transcript covering pnp, the gene encoding polynucleotide phosphorylase, is processed by RNaseIII at the 5'-upstream site of the pnp gene. In the RNaseIII-deficient strain, three species of the unprocessed transcript with different lengths could be detected. In this study, the stability of each transcript was analyzed by SI nuclease protection assay. The results show that the half-lives of the unprocessed transcripts are 8 min, whereas the half-life of the processed transcript is 1.5 min. It is also shown that the 5' segment of the unprocessed transcripts is more stable than the middle or the 3' segment.

MeSH Terms
Endoribonucleases/physiology Escherichia coli/genetics Escherichia coli Proteins Gene Expression Regulation, Bacterial Genes, Bacterial Nucleic Acid Conformation Polyribonucleotide Nucleotidyltransferase/genetics RNA Processing, Post-Transcriptional RNA, Bacterial/genetics RNA, Messenger/genetics,ultrastructure Ribonuclease III Single-Strand Specific DNA and RNA Endonucleases
Chemicals
Escherichia coli Proteins RNA, Bacterial RNA, Messenger Polyribonucleotide Nucleotidyltransferase Endoribonucleases Ribonuclease III ribonuclease III, E coli Single-Strand Specific DNA and RNA Endonucleases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Takata R
Department of Biology, Saga Medical School, Japan.
Izuhara M
Hori K
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/17.18.7441
Published
1989-09-25
Pages
7441-51
Language
English
Region
England
NLM ID
0411011
PMCID
PMC334822
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/17.18.7441 PMC Full Text View journal details
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