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PMID: 2504586 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Two distinct cellular phosphoproteins bind to the c-fos serum response element.

The EMBO journal ·Vol. 8 ·No. 6 ·1989-06-00 ·Pages 1785-92

Ryan WA, Franza BR, Gilman MZ

Abstract

Induction of c-fos transcription by serum growth factors requires a 20 bp DNA sequence termed the serum response element (SRE). The SRE is a binding site for a nuclear protein, serum response factor (SRF). There is as yet no direct evidence that links SRF activity to SRE function. We show here that cells contain a second specific SRE-binding protein. It is distinguished from SRF by its size, chromatographic properties, binding specificity and contacts with nucleotides in the SRE. The presence in cells of two distinct proteins that can bind independently to the SRE suggests that the multiple functions of the SRE may be mediated by distinct proteins.

MeSH Terms
Animals Base Sequence Binding Sites DNA Probes DNA-Binding Proteins/metabolism Humans Mice Phosphoproteins/metabolism Phosphorylation Proto-Oncogene Proteins/genetics,metabolism Proto-Oncogene Proteins c-fos Transcription, Genetic
Chemicals
DNA Probes DNA-Binding Proteins Phosphoproteins Proto-Oncogene Proteins Proto-Oncogene Proteins c-fos
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Ryan W A
Cold Spring Harbor Laboratory, NY 11724.
Franza B R
Gilman M Z
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1989-06-00
Pages
1785-92
Language
English
Region
England
NLM ID
8208664
PMCID
PMC401023
Subset
IM
Grants
NCI NIH HHS · CA 09311 · United States
NCI NIH HHS · CA 40512 · United States
NCI NIH HHS · CA 45642 · United States
External Links
PubMed source PMC Full Text View journal details
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