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PMID: 3320962 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Purification of intercalator-released p67, a polypeptide that interacts specifically with the c-fos serum response element.

Nucleic acids research ·Vol. 15 ·No. 24 ·1987-12-23 ·Pages 10145-58

Schröter H, Shaw PE, Nordheim A

Abstract

Incubation of intact nuclei in buffers containing the DNA intercalating drug chloroquine leads to release of proteins that interact with DNA. We demonstrate here that a protein which binds to a motif within the human c-fos promoter, identified as the serum response element (SRE), is quantitatively released from HeLa nuclei, whereas nuclear factor 1 (NF 1) is not. Purification of the SRE binding protein by affinity chromatography to greater than 95% homogeneity allowed us to identify it as a polypeptide of approximately 67,000 daltons. The DNA contacts made by p67, as identified by methylation interference experiments, are indistinguishable from those of the serum response factor described previously.

MeSH Terms
Binding Sites Chloroquine/pharmacology DNA-Binding Proteins/isolation & purification HeLa Cells Molecular Weight Promoter Regions, Genetic Protein Binding/drug effects Proto-Oncogene Proteins/genetics Regulatory Sequences, Nucleic Acid
Chemicals
DNA-Binding Proteins Proto-Oncogene Proteins Chloroquine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Schröter H
Zentrum für Molekulare Biologie (ZMBH), Universität Heidelberg, FRG.
Shaw P E
Nordheim A
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27 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/15.24.10145
Published
1987-12-23
Pages
10145-58
Language
English
Region
England
NLM ID
0411011
PMCID
PMC339936
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/15.24.10145 PMC Full Text View journal details
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