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PMID: 25093883 Published · ppublish English Journal Article

Microfluidic organs-on-chips.

Nature biotechnology ·Vol. 32 ·No. 8 ·2014-08-00 ·Pages 760-72

Bhatia SN, Ingber DE

Abstract

An organ-on-a-chip is a microfluidic cell culture device created with microchip manufacturing methods that contains continuously perfused chambers inhabited by living cells arranged to simulate tissue- and organ-level physiology. By recapitulating the multicellular architectures, tissue-tissue interfaces, physicochemical microenvironments and vascular perfusion of the body, these devices produce levels of tissue and organ functionality not possible with conventional 2D or 3D culture systems. They also enable high-resolution, real-time imaging and in vitro analysis of biochemical, genetic and metabolic activities of living cells in a functional tissue and organ context. This technology has great potential to advance the study of tissue development, organ physiology and disease etiology. In the context of drug discovery and development, it should be especially valuable for the study of molecular mechanisms of action, prioritization of lead candidates, toxicity testing and biomarker identification.

MeSH Terms
Artificial Organs Bioengineering Biosensing Techniques Caco-2 Cells Drug Design Drug Discovery Humans Microfluidics Models, Biological
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Bhatia Sangeeta N
1] Department of Electrical Engineering &Computer Science, Koch Institute and Institute for Medical Engineering and Science, Massachusetts Institute of Technology and Broad Institute, Cambridge, Massachusetts, USA. [2] Department of Medicine, Brigham and Women's Hospital, Boston, Massachusetts, USA.
Ingber Donald E
1] Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, Massachusetts, USA. [2] Vascular Biology Program, Departments of Pathology &Surgery, Boston Children's Hospital and Harvard Medical School, Boston, Massachusetts, USA. [3] School of Engineering and Applied Sciences, Harvard University, Cambridge, Massachusetts, USA.
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Article Info
Journal
Nature biotechnology
Abbr.
Nat Biotechnol
ISSN
1546-1696
DOI
10.1038/nbt.2989
Published
2014-08-00
Pages
760-72
Language
English
Region
United States
NLM ID
9604648
Subset
IM
External Links
PubMed source DOI: 10.1038/nbt.2989 View journal details
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