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PMID: 2536685 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Rhizobium meliloti fixGHI sequence predicts involvement of a specific cation pump in symbiotic nitrogen fixation.

Journal of bacteriology ·Vol. 171 ·No. 2 ·1989-02-00 ·Pages 929-39

Kahn D, David M, Domergue O, Daveran ML, Ghai J, Hirsch PR, Batut J

Abstract

We present genetic and structural analyses of a fix operon conserved among rhizobia, fixGHI from Rhizobium meliloti. The nucleotide sequence of the operon suggests it may contain a fourth gene, fixS. Adjacent open reading frames of this operon showed an overlap between TGA stop codons and ATG start codons in the form of an ATGA motif suggestive of translational coupling. All four predicted gene products contained probable transmembrane sequences. FixG contained two cysteine clusters typical of iron-sulfur centers and is predicted to be involved in a redox process. FixI was found to be homologous with P-type ATPases, particularly with K+ pumps from Escherichia coli and Streptococcus faecalis but also with eucaryotic Ca2+, Na+/K+, H+/K+, and H+ pumps, which implies that FixI is a pump of a specific cation involved in symbiotic nitrogen fixation. Since prototrophic growth of fixI mutants appeared to be unimpaired, the predicted FixI cation pump probably has a specifically symbiotic function. We suggest that the four proteins FixG, FixH, FixI, and FixS may participate in a membrane-bound complex coupling the FixI cation pump with a redox process catalyzed by FixG.

MeSH Terms
Adenosine Triphosphatases/genetics Amino Acid Sequence Base Sequence DNA Transposable Elements Genes Genes, Bacterial Molecular Sequence Data Multigene Family Nitrogen Fixation Plasmids Restriction Mapping Rhizobium/genetics Symbiosis Transduction, Genetic
Chemicals
DNA Transposable Elements Adenosine Triphosphatases
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Kahn D
Laboratoire de Biologie Moléculaire des Relations Plantes-Microorganismes, Institut National de la Recherche Agronomique, Centre National de la Recherche Scientifique, Castanet-Tolosan, France.
David M
Domergue O
Daveran M L
Ghai J
Hirsch P R
Batut J
References (40)
40 references, click to expand
  1. Cloning of the K+-ATPase of Streptococcus faecalis. Structural and evolutionary implications of its homology to the KdpB-protein of Escherichia coli.
    J Biol Chem. 1987 May 25;262(15):7358-62 PMID: 2953719
  2. A rapid method for the identification of plasmid desoxyribonucleic acid in bacteria.
    Plasmid. 1978 Sep;1(4):584-8 PMID: 107540
  3. The distribution of positively charged residues in bacterial inner membrane proteins correlates with the trans-membrane topology.
    EMBO J. 1986 Nov;5(11):3021-7 PMID: 16453726
  4. Identification of the structural proteins of an ATP-driven potassium transport system in Escherichia coli.
    Proc Natl Acad Sci U S A. 1978 Jul;75(7):3216-9 PMID: 356049
  5. A simple method for displaying the hydropathic character of a protein.
    J Mol Biol. 1982 May 5;157(1):105-32 PMID: 7108955
  6. Cascade regulation of nif gene expression in Rhizobium meliloti.
    Cell. 1988 Aug 26;54(5):671-83 PMID: 2842062
  7. Sequence homology between two membrane transport ATPases, the Kdp-ATPase of Escherichia coli and the Ca2+-ATPase of sarcoplasmic reticulum.
    Proc Natl Acad Sci U S A. 1984 Aug;81(15):4746-50 PMID: 6146979
  8. Rhizobium meliloti nifN (fixF) gene is part of an operon regulated by a nifA-dependent promoter and codes for a polypeptide homologous to the nifK gene product.
    J Bacteriol. 1987 Dec;169(12):5393-400 PMID: 3316182
  9. Location and nucleotide sequence of frdB, the gene coding for the iron-sulphur protein subunit of the fumarate reductase of Escherichia coli.
    Eur J Biochem. 1982 Aug;126(1):211-6 PMID: 6751816
  10. Kilo-sequencing: creation of an ordered nest of asymmetric deletions across a large target sequence carried on phage M13.
    Methods Enzymol. 1983;101:98-122 PMID: 6310345
  11. Generalized transduction in Rhizobium meliloti.
    J Bacteriol. 1984 Jul;159(1):125-9 PMID: 6330025
  12. Principle of codification for quick comparisons with the entire biomolecule databanks and associated programs in FORTRAN 77.
    Nucleic Acids Res. 1986 Jan 10;14(1):197-204 PMID: 3753764
  13. Amino-acid sequence of a Ca2+ + Mg2+-dependent ATPase from rabbit muscle sarcoplasmic reticulum, deduced from its complementary DNA sequence.
    Nature. 1985 Aug 22-28;316(6030):696-700 PMID: 2993904
  14. A new symbiotic cluster on the pSym megaplasmid of Rhizobium meliloti 2011 carries a functional fix gene repeat and a nod locus.
    J Bacteriol. 1987 May;169(5):2231-8 PMID: 3571166
  15. Amino-acid sequence of the catalytic subunit of the (Na+ + K+)ATPase deduced from a complementary DNA.
    Nature. 1985 Aug 22-28;316(6030):691-5 PMID: 2993903
  16. Mapping and expression of a regulatory nitrogen fixation gene (fixD) of Rhizobium meliloti.
    EMBO J. 1985 Nov;4(11):2751-6 PMID: 16453640
  17. The genetic complexity of nitrogen fixation. The ninth Fleming lecture.
    J Gen Microbiol. 1984 Nov;130(11):2745-55 PMID: 6396374
  18. Molecular cloning of the rat stomach (H+ + K+)-ATPase.
    J Biol Chem. 1986 Dec 25;261(36):16788-91 PMID: 3023364
  19. Conservation of symbiotic nitrogen fixation gene sequences in Rhizobium japonicum and Bradyrhizobium japonicum.
    J Bacteriol. 1985 Jul;163(1):21-6 PMID: 4008441
  20. Analysis of membrane and surface protein sequences with the hydrophobic moment plot.
    J Mol Biol. 1984 Oct 15;179(1):125-42 PMID: 6502707
  21. Use of the 'Perceptron' algorithm to distinguish translational initiation sites in E. coli.
    Nucleic Acids Res. 1982 May 11;10(9):2997-3011 PMID: 7048259
  22. Megaplasmids in the plant-associated bacteria Rhizobium meliloti and Pseudomonas solanacearum.
    J Bacteriol. 1982 Apr;150(1):402-6 PMID: 7061401
  23. Nucleotide sequence of bacteriophage lambda DNA.
    J Mol Biol. 1982 Dec 25;162(4):729-73 PMID: 6221115
  24. Plasmalemma redox activity and h extrusion: I. Activation of the h-pump by ferricyanide-induced potential depolarization and cytoplasm acidification.
    Plant Physiol. 1988 May;87(1):25-9 PMID: 16666112
  25. Genetic manipulations in Rhizobium meliloti utilizing two new transposon Tn5 derivatives.
    Mol Gen Genet. 1986 Sep;204(3):485-91 PMID: 3020382
  26. Transcription patterns of Rhizobium meliloti symbiotic plasmid pSym: identification of nifA-independent fix genes.
    J Bacteriol. 1987 May;169(5):2239-44 PMID: 2437100
  27. Characterization of translational initiation sites in E. coli.
    Nucleic Acids Res. 1982 May 11;10(9):2971-96 PMID: 7048258
  28. Nucleotide sequence of a 24,206-base-pair DNA fragment carrying the entire nitrogen fixation gene cluster of Klebsiella pneumoniae.
    J Mol Biol. 1988 Oct 5;203(3):715-38 PMID: 3062178
  29. Primary structure of the alpha-subunit of Torpedo californica (Na+ + K+)ATPase deduced from cDNA sequence.
    Nature. 1985 Aug 22-28;316(6030):733-6 PMID: 2993905
  30. A Rhizobium meliloti symbiotic regulatory gene.
    Cell. 1984 Apr;36(4):1035-43 PMID: 6423287
  31. Tertiary structure of Bacillus thermoproteolyticus [4Fe-4S] ferredoxin. Evolutionary implications for bacterial ferredoxins.
    J Mol Biol. 1988 Jan 5;199(1):183-93 PMID: 3351918
  32. Establishing homologies in protein sequences.
    Methods Enzymol. 1983;91:524-45 PMID: 6855599
  33. Relationship of Transplasmalemma Redox Activity to Proton and Solute Transport by Roots of Zea mays.
    Plant Physiol. 1986 Apr;80(4):805-11 PMID: 16664722
  34. Conservation of structure and location of Rhizobium meliloti and Klebsiella pneumoniae nifB genes.
    J Bacteriol. 1987 Mar;169(3):1120-6 PMID: 3029020
  35. Nucleotide sequence encoding the iron-sulphur protein subunit of the succinate dehydrogenase of Escherichia coli.
    Biochem J. 1984 Oct 15;223(2):507-17 PMID: 6388571
  36. Genetic and structural analysis of the Rhizobium meliloti fixA, fixB, fixC, and fixX genes.
    J Bacteriol. 1987 Mar;169(3):1127-36 PMID: 3029021
  37. A general method for site-directed mutagenesis in prokaryotes.
    Nature. 1981 Jan 1;289(5793):85-8 PMID: 6256652
  38. Yeast plasma membrane ATPase is essential for growth and has homology with (Na+ + K+), K+- and Ca2+-ATPases.
    Nature. 1986 Feb 20-26;319(6055):689-93 PMID: 3005867
  39. Characterization of three genomic loci encoding Rhizobium sp. strain ORS571 N2 fixation genes.
    J Bacteriol. 1986 Jan;165(1):72-81 PMID: 3001035
  40. Directed transposon Tn5 mutagenesis and complementation analysis of Rhizobium meliloti symbiotic nitrogen fixation genes.
    Cell. 1982 Jun;29(2):551-9 PMID: 6288262
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.171.2.929-939.1989
Published
1989-02-00
Pages
929-39
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC209684
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.171.2.929-939.1989 PMC Full Text View journal details
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