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PMID: 2539209 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Multiple conductance states of the purified calcium release channel complex from skeletal sarcoplasmic reticulum.

Biophysical journal ·Vol. 55 ·No. 3 ·1989-03-00 ·Pages 415-24

Liu QY, Lai FA, Rousseau E, Jones RV, Meissner G

Abstract

The CHAPS-solubilized and purified 30S ryanodine receptor protein complex from skeletal sarcoplasmic reticulum (SR) was incorporated into planar lipid bilayers. The resulting electrical activity displayed similar responses to agents such as Ca2+, ATP, ryanodine, or caffeine as the native Ca2+ release channel, confirming the identification of the 30S complex as the Ca2+ release channel. The purified channel was permeable to monovalent ions such as Na+, with the permeability ratio PCa/PNa approximately 5, and was highly selective for cations over anions. The purified channel also showed at least four distinct conductance levels for both Na+ and Ca2+ conducting ions, with the major subconducting level in NaCl buffers possessing half the conductance value of the main conductance state. These levels may be produced by intrinsic subconductances present within the channel oligomer. Several of these conductances may be cooperatively coupled to produce the characteristic 100 +/- 10 pS unitary Ca2+ conductance of the native channel.

MeSH Terms
Animals Calcium/pharmacology Calcium Channels/drug effects,physiology Egtazic Acid/pharmacology Electric Conductivity Kinetics Lipid Bilayers Muscles/physiology Receptors, Cholinergic/drug effects,isolation & purification,metabolism,physiology Ryanodine Receptor Calcium Release Channel Sarcoplasmic Reticulum/physiology
Chemicals
Calcium Channels Lipid Bilayers Receptors, Cholinergic Ryanodine Receptor Calcium Release Channel Egtazic Acid Calcium
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Liu Q Y
Department of Biochemistry, University of North Carolina School of Medicine, Chapel Hill 27599-7260.
Lai F A
Rousseau E
Jones R V
Meissner G
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32 references, click to expand
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Article Info
Journal
Biophysical journal
Abbr.
Biophys J
ISSN
0006-3495
DOI
10.1016/S0006-3495(89)82835-8
Published
1989-03-00
Pages
415-24
Language
English
Region
United States
NLM ID
0370626
PMCID
PMC1330495
Subset
IM
Grants
NIAMS NIH HHS · AR18687 · United States
External Links
PubMed source DOI: 10.1016/S0006-3495(89)82835-8 PMC Full Text View journal details
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