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PMID: 2542231 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Overproduction of transposon Tn10-encoded tetracycline resistance protein results in cell death and loss of membrane potential.

Journal of bacteriology ·Vol. 171 ·No. 6 ·1989-06-00 ·Pages 3557-9

Eckert B, Beck CF

Abstract

High-level expression of the Tn10 tetracycline resistance protein TetA in Escherichia coli caused partial collapse of the membrane potential, arrest of growth, and killing of the cells. Since alpha-methylglucoside transport was not affected, the overproduced TetA protein may cause not destruction of membrane structure but rather unrestricted translocation of protons and/or ions across the membrane.

MeSH Terms
Bacterial Proteins/physiology Cell Survival DNA Transposable Elements Escherichia coli/genetics Gene Expression Regulation Membrane Potentials Methylglucosides/metabolism Proline/metabolism Tetracycline/metabolism Tetracycline Resistance
Chemicals
Bacterial Proteins DNA Transposable Elements Methylglucosides methylglucoside Proline Tetracycline
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Eckert B
Institut für Biologie III, Albert-Ludwigs-Universität Freiburg, Federal Republic of Germany.
Beck C F
References (21)
21 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.171.6.3557-3559.1989
Published
1989-06-00
Pages
3557-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC210086
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.171.6.3557-3559.1989 PMC Full Text View journal details
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