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PMID: 25467443 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Species-wide genetic incompatibility analysis identifies immune genes as hot spots of deleterious epistasis.

Cell ·Vol. 159 ·No. 6 ·2014-12-04 ·Pages 1341-51

Chae E, Bomblies K, Kim ST, Karelina D, Zaidem M, Ossowski S, Martín-Pizarro C, Laitinen RA, Rowan BA, Tenenboim H, Lechner S, Demar M, Habring-Müller A, Lanz C, Rätsch G, Weigel D

Abstract

Intraspecific genetic incompatibilities prevent the assembly of specific alleles into single genotypes and influence genome- and species-wide patterns of sequence variation. A common incompatibility in plants is hybrid necrosis, characterized by autoimmune responses due to epistatic interactions between natural genetic variants. By systematically testing thousands of F1 hybrids of Arabidopsis thaliana strains, we identified a small number of incompatibility hot spots in the genome, often in regions densely populated by nucleotide-binding domain and leucine-rich repeat (NLR) immune receptor genes. In several cases, these immune receptor loci interact with each other, suggestive of conflict within the immune system. A particularly dangerous locus is a highly variable cluster of NLR genes, DM2, which causes multiple independent incompatibilities with genes that encode a range of biochemical functions, including NLRs. Our findings suggest that deleterious interactions of immune receptors limit the combinations of favorable disease resistance alleles accessible to plant genomes.

MeSH Terms
Amino Acid Sequence Arabidopsis/classification,genetics,immunology Crosses, Genetic Epistasis, Genetic Genome, Plant Hybridization, Genetic Molecular Sequence Data Phylogeny Plant Physiological Phenomena Sequence Alignment
Authors & Affiliations
16 authors, click to expand affiliations / ORCID
Chae Eunyoung
Department of Molecular Biology, Max Planck Institute for Developmental Biology, 72076 Tübingen, Germany.
Bomblies Kirsten
Department of Molecular Biology, Max Planck Institute for Developmental Biology, 72076 Tübingen, Germany.
Kim Sang-Tae
Department of Molecular Biology, Max Planck Institute for Developmental Biology, 72076 Tübingen, Germany.
Karelina Darya
Department of Molecular Biology, Max Planck Institute for Developmental Biology, 72076 Tübingen, Germany; Friedrich Miescher Laboratory, Max Planck Society, 72076 Tübingen, Germany.
Zaidem Maricris
Department of Molecular Biology, Max Planck Institute for Developmental Biology, 72076 Tübingen, Germany.
Ossowski Stephan
Department of Molecular Biology, Max Planck Institute for Developmental Biology, 72076 Tübingen, Germany.
Martín-Pizarro Carmen
Department of Molecular Biology, Max Planck Institute for Developmental Biology, 72076 Tübingen, Germany.
Laitinen Roosa A E
Department of Molecular Biology, Max Planck Institute for Developmental Biology, 72076 Tübingen, Germany.
Rowan Beth A
Department of Molecular Biology, Max Planck Institute for Developmental Biology, 72076 Tübingen, Germany.
Tenenboim Hezi
Department of Molecular Biology, Max Planck Institute for Developmental Biology, 72076 Tübingen, Germany.
Lechner Sarah
Department of Molecular Biology, Max Planck Institute for Developmental Biology, 72076 Tübingen, Germany.
Demar Monika
Department of Molecular Biology, Max Planck Institute for Developmental Biology, 72076 Tübingen, Germany.
Habring-Müller Anette
Department of Molecular Biology, Max Planck Institute for Developmental Biology, 72076 Tübingen, Germany.
Lanz Christa
Department of Molecular Biology, Max Planck Institute for Developmental Biology, 72076 Tübingen, Germany.
Rätsch Gunnar
Friedrich Miescher Laboratory, Max Planck Society, 72076 Tübingen, Germany.
Weigel Detlef
Department of Molecular Biology, Max Planck Institute for Developmental Biology, 72076 Tübingen, Germany. Electronic address: [email protected].
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Article Info
Journal
Cell
Abbr.
Cell
ISSN
1097-4172
DOI
10.1016/j.cell.2014.10.049
Email
[email protected]
Published
2014-12-04
Epub
2014-00-20
Pages
1341-51
Language
English
Region
United States
NLM ID
0413066
PMCID
PMC4269942
Subset
IM
Grants
NIGMS NIH HHS · F32 GM075741 · United States
Databases
GENBANK
KJ454428, KJ634210, KJ634211
Corrections
CommentIn
PMID: 25480288
External Links
PubMed source DOI: 10.1016/j.cell.2014.10.049 PMC Full Text View journal details
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