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PMID: 2564662 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Expression of Hox-2.4 homeobox gene directed by proviral insertion in a myeloid leukemia.

Nucleic acids research ·Vol. 17 ·No. 5 ·1989-03-11 ·Pages 1881-92

Kongsuwan K, Allen J, Adams JM

Abstract

The presence of an altered Hox-2.4 gene in the WEHI3B murine myeloid leukemia suggests that homeobox genes may contribute to neoplasia. A survey of 31 leukemia cell lines of the myeloid, lymphoid and erythroid lineages revealed that Hox-2.4 was expressed only in WEHI3B and the pre-B lymphoid line 70Z/3, in which no DNA rearrangement was observed. To clarify the WEHI3B alteration and normal Hox-2.4 structure, we have sequenced near full length cDNA clones from WEHI3B and 70Z/3, and the 5' portion of the normal Hox-2.4 gene. A WEHI3B cDNA clone demonstrates that an intracisternal A-particle (IAP) provirus has inserted within the first exon of the gene and generated a Hox-2.4 mRNA with a 5' sequence derived from the IAP long terminal repeat. A remarkable degree of similarity found between the amino acid sequences of Hox-2.4 and Hox-3.1, which reside on different chromosomes, supports the notion that an ancient homeobox gene cluster has been duplicated and dispersed early in vertebrate evolution.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cell Line Gene Expression Regulation Genes, Homeobox Genes, Intracisternal A-Particle Hematopoietic Stem Cells/analysis Leukemia, Myeloid/genetics Mice Mice, Inbred BALB C Molecular Sequence Data RNA, Messenger/isolation & purification Sequence Homology, Nucleic Acid Viral Proteins/genetics,isolation & purification
Chemicals
RNA, Messenger Viral Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kongsuwan K
Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria, Australia.
Allen J
Adams J M
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/17.5.1881
Published
1989-03-11
Pages
1881-92
Language
English
Region
England
NLM ID
0411011
PMCID
PMC317530
Subset
IM
Grants
NCI NIH HHS · CA12421 · United States
Databases
GENBANK
X13721
External Links
PubMed source DOI: 10.1093/nar/17.5.1881 PMC Full Text View journal details
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