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PMID: 2907477 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

DNA rearrangement of a homeobox gene in myeloid leukaemic cells.

The EMBO journal ·Vol. 7 ·No. 13 ·1988-12-20 ·Pages 4283-90

Blatt C, Aberdam D, Schwartz R, Sachs L

Abstract

A homeobox gene rearrangement has been detected in WEHI-3B mouse myeloid leukaemic cells. The rearranged gene was identified as Hox-2.4 which is a member of the Hox-2 gene cluster on mouse chromosome 11. Both the normal and the rearranged genes were cloned and analysed, and the rearranged genomic Hox-2.4 gene was sequenced. The results indicate that the rearrangement is due to insertion of an intracisternal A particle 5' upstream to Hox-2.4 and that this resulted in constitutive expression of the homeobox gene. It is suggested that constitutive expression of the homeobox gene may interrupt the normal development program in these leukaemic cells.

MeSH Terms
Amino Acid Sequence Animals Base Sequence DNA, Neoplasm/genetics Gene Expression Regulation Gene Rearrangement Genes, Homeobox Genes, Intracisternal A-Particle Leukemia, Myeloid/genetics Mice Molecular Sequence Data Restriction Mapping Tumor Cells, Cultured
Chemicals
DNA, Neoplasm
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Blatt C
Department of Genetics, Weizmann Institute of Science, Rehovot, Israel.
Aberdam D
Schwartz R
Sachs L
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1988-12-20
Pages
4283-90
Language
English
Region
England
NLM ID
8208664
PMCID
PMC455142
Subset
IM
Corrections
ErratumIn
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External Links
PubMed source PMC Full Text View journal details
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