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PMID: 6953429 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Isolation and characterization of rat skeletal muscle and cytoplasmic actin genes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 9 ·1982-05-00 ·Pages 2763-7

Nudel U, Katcoff D, Zakut R, Shani M, Carmon Y, Finer M, Czosnek H, Ginsburg I, Yaffe D

Abstract

Southern blots of rat genomic DNA indicate the existence of at least 12 EcoRI DNA fragments containing actin gene sequences. By using specific probes and stringent conditions of hybridization, it was found that only one of these fragments contains sequences of the skeletal muscle alpha-actin gene. Recombinant bacteriophages originating from eight different actin genes were isolated from rat genomic DNA libraries. One of them, Act 15, contains the skeletal muscle actin gene. Another clone, Act I, contains a gene coding for a cytoplasmic actin, identified tentatively as the beta-actin gene. Both genes have a large intron very close to the 5' end of their transcribed region, followed by several small introns. DNA sequence analysis and comparison with the available data on actin genes in other organisms indicated an interesting relationship between the positions of introns and the evolutionary relatedness. Several intron sites are conserved from at least the echinoderms to the vertebrates; others appear to be present in some actin genes and not in others.

MeSH Terms
Actins/genetics Animals Base Sequence Biological Evolution Cytoplasm/physiology Genes Muscles/physiology Nucleic Acid Hybridization Rats
Chemicals
Actins
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Nudel U
Katcoff D
Zakut R
Shani M
Carmon Y
Finer M
Czosnek H
Ginsburg I
Yaffe D
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.79.9.2763
Published
1982-05-00
Pages
2763-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC346286
Subset
IM
Grants
NIGMS NIH HHS · R01 GM 22767 · United States
External Links
PubMed source DOI: 10.1073/pnas.79.9.2763 PMC Full Text View journal details
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