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PMID: 6777773 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Organization of actin gene sequences in the sea urchin: molecular cloning of an intron-containing DNA sequence coding for a cytoplasmic actin.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 77 ·No. 10 ·1980-10-00 ·Pages 5683-7

Durica DS, Schloss JA, Crain WR

Abstract

Southern transfer and solution hybridization experiments, using as probe a DNA fragment that encodes for Drosophila actin, demonstrate cross hybridization to DNA from the sea urchin Strongylocentrotus purpuratus. Recombinant DNA clones that contained sea urchin genomic DNA fragments were constructed and screened for the presence of actin-encoding DNA sequences by colony hybridization with the Drosophila actin sequence. Two different putative actin-encoding clones were identified and were shown to specifically hybridize actin-encoding mRNA from a complex mRNA population. Southern blot hybridization experiments with both the Drosophila actin sequence and one of the cloned sea urchin sequences, in conjunction with solution hybridization data, suggest an actin gene copy number of 5-20 per haploid genome. DNA sequence analysis of one of the cloned sequences indicates that this fragment codes for a cytoplasmic form of actin and contains an intervening sequence of at least 200 nucleotides beginning immediately after amino acid 121 in the protein sequence.

MeSH Terms
Actins/genetics Animals Cloning, Molecular DNA, Recombinant Drosophila melanogaster/genetics Genes Isoelectric Point Molecular Weight Sea Urchins/genetics
Chemicals
Actins DNA, Recombinant
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Durica D S
Schloss J A
Crain W R
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31 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.77.10.5683
Published
1980-10-00
Pages
5683-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC350133
Subset
IM
Grants
NIGMS NIH HHS · GM 24620 · United States
NIGMS NIH HHS · GM 25492 · United States
NCI NIH HHS · P30 CA12708 · United States
Databases
GENBANK
J01201
External Links
PubMed source DOI: 10.1073/pnas.77.10.5683 PMC Full Text View journal details
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