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PMID: 2572668 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Induction of human IgE synthesis by CD4+ T cell clones. Requirement for interleukin 4 and low molecular weight B cell growth factor.

The Journal of experimental medicine ·Vol. 170 ·No. 5 ·1989-11-01 ·Pages 1477-93

DeKruyff RH, Turner T, Abrams JS, Palladino MA, Umetsu DT

Abstract

We have analyzed in detail the precise requirements for the induction of human IgE synthesis using several experimental approaches with purified B cells and well-characterized alloantigen-specific CD4+ T cell clones expressing different profiles of lymphokine secretion. Using these clones under cognate conditions in which the B cells expressed alloantigens recognized by the cloned T cells, we have confirmed that IL-4 is required for the induction of IgE synthesis, but we have clearly demonstrated that IL-4 by itself is not sufficient. With several cloned CD4+ T cell lines, including an IL-4-producing clone that could not induce IgE synthesis, and cloned T cells pretreated with cyclosporin A to inhibit lymphokine synthesis, we showed that Th cell-B cell interactions are necessary for IgE synthesis, and that low molecular weight B cell growth factor (LMW-BCGF) and IL-4, in combination, are lymphokines of major importance in the induction of IgE synthesis. Together our results indicate that optimal induction of an IgE-specific response requires the exposure of B cells to a particular complex of signals that include (a) a signal(s) involving Th-B cell interaction that primes B cells to receive additional signals from soluble lymphokines, (b) a specific B cell proliferative signal provided by LMW-BCGF, and (c) a specific B cell differentiation signal provided by IL-4.

MeSH Terms
CD4-Positive T-Lymphocytes/immunology Clone Cells Cyclosporins/pharmacology Humans Immunoglobulin E/biosynthesis In Vitro Techniques Interleukin-2/pharmacology Interleukin-4/physiology Lymphocyte Cooperation Molecular Weight T-Lymphocytes, Helper-Inducer/immunology
Chemicals
Cyclosporins Interleukin-2 Interleukin-4 Immunoglobulin E
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
DeKruyff R H
Department of Pediatrics, Stanford University School of Medicine, California 94305.
Turner T
Abrams J S
Palladino M A
Umetsu D T
References (54)
54 references, click to expand
  1. Suppression of in vivo polyclonal IgE responses by monoclonal antibody to the lymphokine B-cell stimulatory factor 1.
    Proc Natl Acad Sci U S A. 1986 Dec;83(24):9675-8 PMID: 3491987
  2. Mechanism of interleukin-2 signaling: mediation of different outcomes by a single receptor and transduction pathway.
    Science. 1989 Feb 10;243(4892):781-6 PMID: 2492678
  3. Cloned, Ia-restricted T cells that do not produce interleukin 4(IL 4)/B cell stimulatory factor 1(BSF-1) fail to help antigen-specific B cells.
    J Immunol. 1987 Mar 15;138(6):1674-9 PMID: 2950166
  4. Molecular cloning and expression of a human B-cell growth factor gene in Escherichia coli.
    Science. 1987 Mar 20;235(4795):1489-92 PMID: 3547651
  5. Interferon-gamma and B cell stimulatory factor-1 reciprocally regulate Ig isotype production.
    Science. 1987 May 22;236(4804):944-7 PMID: 3107127
  6. Induction of human IgE synthesis requires interleukin 4 and T/B cell interactions involving the T cell receptor/CD3 complex and MHC class II antigens.
    J Exp Med. 1989 Apr 1;169(4):1295-307 PMID: 2522501
  7. Induction of antigen-specific antibody responses in primed and unprimed B cells. Functional heterogeneity among Th1 and Th2 T cell clones.
    J Immunol. 1989 Apr 15;142(8):2575-82 PMID: 2522962
  8. 'B-cell factors' are pleiotropic.
    Immunol Today. 1988 Feb;9(2):45-54 PMID: 3151436
  9. Species-specificity of T cell stimulating activities of IL 2 and BSF-1 (IL 4): comparison of normal and recombinant, mouse and human IL 2 and BSF-1 (IL 4).
    J Immunol. 1987 Mar 15;138(6):1813-6 PMID: 3493289
  10. Identification of a T cell-derived b cell growth factor distinct from interleukin 2.
    J Exp Med. 1982 Mar 1;155(3):914-23 PMID: 6977612
  11. Dissociation of two signals required for activation of resting B cells.
    Proc Natl Acad Sci U S A. 1982 Mar;79(6):1989-93 PMID: 6979046
  12. Induction of human IgE synthesis by a factor derived from T cells of patients with hyper-IgE states.
    J Immunol. 1983 Jan;130(1):242-7 PMID: 6292303
  13. Role of the major histocompatibility complex in T cell activation of B cell subpopulations. Ir gene regulation of the T cell-dependent activation of distinct B cell subpopulations.
    J Immunol. 1983 Jan;130(1):67-71 PMID: 6401188
  14. Development of a human T-T cell hybridoma secreting B cell growth factor.
    J Exp Med. 1983 Jan 1;157(1):60-8 PMID: 6600272
  15. Helper signals in the plaque-forming cell response to protein-bound haptens.
    J Exp Med. 1983 Aug 1;158(2):317-33 PMID: 6193219
  16. A cloned major histocompatibility complex-restricted trinitrophenyl-reactive human helper T cell line that activates B cell subsets via two distinct pathways.
    J Exp Med. 1983 Nov 1;158(5):1444-58 PMID: 6195284
  17. One out of five peripheral blood B lymphocytes is activated to high-rate Ig production by human alloreactive T cell clones.
    Eur J Immunol. 1983 Oct;13(10):820-4 PMID: 6227488
  18. Detection and functional studies of p60-65 (Tac antigen) on activated human B cells.
    J Exp Med. 1984 Nov 1;160(5):1597-602 PMID: 6092512
  19. Human interleukin 2 can promote the growth and differentiation of single hapten-specific B cells in the presence of specific antigen.
    Proc Natl Acad Sci U S A. 1984 Dec;81(24):7917-21 PMID: 6334854
  20. Interleukin 2 receptors on human B cells. Implications for the role of interleukin 2 in human B cell function.
    J Exp Med. 1985 Jan 1;161(1):181-97 PMID: 2981952
  21. B-cell growth factor (B-cell growth factor I or B-cell-stimulating factor, provisional 1) is a differentiation factor for resting B cells and may not induce cell growth.
    Proc Natl Acad Sci U S A. 1985 Apr;82(8):2465-7 PMID: 3873068
  22. Pneumococcal polysaccharides induce antibody formation by human B lymphocytes in vitro.
    J Immunol. 1985 Jul;135(1):1-4 PMID: 3889152
  23. Differential requirements of B cells from normal and allergic subjects for the induction of IgE synthesis by an alloreactive T cell clone.
    J Exp Med. 1985 Jul 1;162(1):202-14 PMID: 3874259
  24. Regulation of the IgM and IgA anti-dextran B1355S response: synergy between IFN-gamma, BCGF II, and IL 2.
    J Immunol. 1985 Dec;135(6):4015-20 PMID: 2415594
  25. A T cell activity that enhances polyclonal IgE production and its inhibition by interferon-gamma.
    J Immunol. 1986 Feb 1;136(3):949-54 PMID: 2934482
  26. Phase I trial of recombinant interferon gamma in cancer patients.
    J Clin Oncol. 1986 Feb;4(2):137-46 PMID: 3080551
  27. Induction of IgG and IgE synthesis in normal B cells by autoreactive T cell clones.
    J Immunol. 1986 Apr 15;136(8):2851-5 PMID: 2937838
  28. B cell stimulatory factor-1 enhances the IgE response of lipopolysaccharide-activated B cells.
    J Immunol. 1986 Jun 15;136(12):4538-41 PMID: 3486902
  29. Serum-free in vitro bioassay for the detection of tumor necrosis factor.
    J Immunol Methods. 1986 Nov 6;93(2):201-6 PMID: 3772113
  30. Antigen presentation by hapten-specific B lymphocytes. III. Analysis of the immunoglobulin-dependent pathway of antigen presentation to interleukin 1-dependent T lymphocytes.
    Eur J Immunol. 1986 Nov;16(11):1407-12 PMID: 2946591
  31. B cell growth-promoting activity of recombinant human interleukin 4.
    J Immunol. 1987 Aug 15;139(4):1135-41 PMID: 3112225
  32. Regulation of murine IgE production in SJA/9 and nude mice. Potentiation of IgE production by recombinant interleukin 4.
    J Immunol. 1987 Oct 15;139(8):2538-44 PMID: 3498762
  33. Heterogeneity among T helper cells. Interleukin 2 secretion and help for immunoglobulin secretion.
    J Immunol. 1987 Oct 15;139(8):2567-72 PMID: 2958555
  34. Synergistic interaction between interleukin 4 and anti-Bp50 (CDw40) revealed in a novel B cell restimulation assay.
    Eur J Immunol. 1987 Oct;17(10):1535-8 PMID: 3500063
  35. A mouse T cell product that preferentially enhances IgA production. I. Biologic characterization.
    J Immunol. 1987 Dec 1;139(11):3685-90 PMID: 2960739
  36. Lymphotoxin is an important T cell-derived growth factor for human B cells.
    Science. 1987 Nov 20;238(4830):1144-6 PMID: 3500512
  37. Induction of lymphokine responsiveness of hapten-specific B lymphocytes promoted through an antigen-mediated T helper lymphocyte interaction.
    J Immunol. 1988 Jan 15;140(2):367-75 PMID: 2447174
  38. Differential regulation of IgG1 and IgE synthesis by interleukin 4.
    J Exp Med. 1988 Jan 1;167(1):183-96 PMID: 3257252
  39. T cell-dependent hapten-specific and polyclonal B cell responses require release of interleukin 5.
    J Immunol. 1988 Feb 1;140(3):705-12 PMID: 2448371
  40. The essential role of B cell stimulatory factor 2 (BSF-2/IL-6) for the terminal differentiation of B cells.
    J Exp Med. 1988 Feb 1;167(2):332-44 PMID: 3258006
  41. Receptor-directed focusing of lymphokine release by helper T cells.
    Nature. 1988 Mar 24;332(6162):378-80 PMID: 2965306
  42. IL-4 is an essential factor for the IgE synthesis induced in vitro by human T cell clones and their supernatants.
    J Immunol. 1988 Jun 15;140(12):4193-8 PMID: 2967330
  43. Functional heterogeneity among human inducer T cell clones.
    J Immunol. 1988 Jun 15;140(12):4211-6 PMID: 2967331
  44. Interleukin 5 enhances interleukin 4-induced IgE production by normal human B cells. The role of soluble CD23 antigen.
    Eur J Immunol. 1988 Jun;18(6):929-35 PMID: 3260186
  45. Recognition by a human alloreactive T-cell clone of an HLA-DP-associated epitope on monocytes and fibroblasts but not on B cells.
    J Clin Immunol. 1988 May;8(3):163-70 PMID: 2455729
  46. Simultaneous production of IL-2, IL-4, and IFN-gamma by activated human CD4+ and CD8+ T cell clones.
    J Immunol. 1988 Aug 1;141(3):849-55 PMID: 2969394
  47. IgE production by normal human B cells induced by alloreactive T cell clones is mediated by IL-4 and suppressed by IFN-gamma.
    J Immunol. 1988 Aug 15;141(4):1218-24 PMID: 3135324
  48. Regulation of antibody isotype secretion by subsets of antigen-specific helper T cells.
    Nature. 1988 Jul 21;334(6179):255-8 PMID: 2456466
  49. Profiles of lymphokine activities and helper function for IgE in human T cell clones.
    Eur J Immunol. 1988 Jul;18(7):1045-50 PMID: 2969818
  50. IgE production by normal human lymphocytes is induced by interleukin 4 and suppressed by interferons gamma and alpha and prostaglandin E2.
    Proc Natl Acad Sci U S A. 1988 Sep;85(18):6880-4 PMID: 2970644
  51. IL-4 is required to generate and sustain in vivo IgE responses.
    J Immunol. 1988 Oct 1;141(7):2335-41 PMID: 2459206
  52. Interleukin 4 causes isotype switching to IgE in T cell-stimulated clonal B cell cultures.
    J Exp Med. 1988 Sep 1;168(3):853-62 PMID: 3049907
  53. Clonal analysis of functionally distinct human CD4+ T cell subsets.
    J Exp Med. 1988 Nov 1;168(5):1659-73 PMID: 2903211
  54. Receptors for B-cell stimulatory factor-1 expressed on cells of haematopoietic lineage.
    Nature. 1987 Feb 5-11;325(6104):537-40 PMID: 3100961
Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.170.5.1477
Published
1989-11-01
Pages
1477-93
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2189506
Subset
IM
Grants
NIAID NIH HHS · AI-24571 · United States
NIAID NIH HHS · AI-26322 · United States
NCRR NIH HHS · RR-05353 · United States
External Links
PubMed source DOI: 10.1084/jem.170.5.1477 PMC Full Text View journal details
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