Home LiteratureArticle Details
PMID: 2579101 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Human macrophage activation. Modulation of mannosyl, fucosyl receptor activity in vitro by lymphokines, gamma and alpha interferons, and dexamethasone.

The Journal of clinical investigation ·Vol. 75 ·No. 2 ·1985-02-00 ·Pages 624-31

Mokoena T, Gordon S

Abstract

We describe a sensitive assay to measure immune activation of human macrophages in cell culture. Freshly isolated blood monocytes from normal subjects lack the ability to endocytose and degrade mannosyl-terminated glycoconjugates via specific receptors, but acquired this activity after cultivation in autologous serum for approximately 3 d. Addition of specific antigen, purified protein derivative, or T cell mitogens to mononuclear cells prevented the appearance of macrophage mannosyl receptor activity and lymphokine, gamma-, and alpha-interferons selectively down-regulated receptor activity in monocyte-macrophage targets. The effects of antigen challenge and gamma-interferon on mannosyl receptors can be prevented by 10(-8) M dexamethasone. Dexamethasone also inhibited release of another macrophage activation marker, plasminogen activator, which was increased by both gamma- and alpha-interferons. These studies show that activation of human macrophages is regulated by opposing actions of lymphokines and glucocorticoids.

MeSH Terms
Adult Antigens/immunology Dexamethasone/pharmacology Fibrinolysis/drug effects Humans In Vitro Techniques Interferons/pharmacology Lectins, C-Type Lymphocyte Activation Lymphokines/pharmacology Macrophage Activation/drug effects Mannose Receptor Mannose-Binding Lectins Mitogens/pharmacology Monocytes/physiology Receptors, Cell Surface Receptors, Immunologic/drug effects,metabolism
Chemicals
Antigens Lectins, C-Type Lymphokines Mannose Receptor Mannose-Binding Lectins Mitogens Receptors, Cell Surface Receptors, Immunologic Dexamethasone Interferons
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Mokoena T
Gordon S
References (37)
37 references, click to expand
  1. Glucocorticoid suppression of macrophage migration inhibitory factor.
    J Exp Med. 1973 Apr 1;137(4):1031-41 PMID: 4693151
  2. A unique set of polypeptides is induced by gamma interferon in addition to those induced in common with alpha and beta interferons.
    Nature. 1983 Feb 3;301(5899):437-9 PMID: 6218412
  3. Macrophage plasminogen activator: modulation of enzyme production by anti-inflammatory steroids, mitotic inhibitors, and cyclic nucleotides.
    Cell. 1976 Jun;8(2):271-81 PMID: 61067
  4. Evidence for receptor-mediated binding of glycoproteins, glycoconjugates, and lysosomal glycosidases by alveolar macrophages.
    Proc Natl Acad Sci U S A. 1978 Mar;75(3):1399-403 PMID: 274729
  5. Activation of mononuclear phagocytes: fact, fancy, and future.
    J Immunol. 1978 Sep;121(3):813-6 PMID: 357655
  6. Secretion of plasminogen activator by bone marrow-derived mononuclear phagocytes and its enhancement by colony-stimulating factor.
    J Exp Med. 1979 Aug 1;150(2):231-45 PMID: 313429
  7. Plasminogen activator production by human monocytes. I. Enhancement by activated lymphocytes and lymphocyte products.
    J Immunol. 1979 Dec;123(6):2808-13 PMID: 501090
  8. Macrophage oxygen-dependent antimicrobial activity. III. Enhanced oxidative metabolism as an expression of macrophage activation.
    J Exp Med. 1980 Dec 1;152(6):1596-609 PMID: 6256463
  9. Interferon induced in human leukocytes by mitogens: production, partial purification and characterization.
    Eur J Immunol. 1980 Nov;10(11):877-83 PMID: 6161829
  10. Surface properties of bacillus Calmette-Guérin-activated mouse macrophages. Reduced expression of mannose-specific endocytosis, Fc receptors, and antigen F4/80 accompanies induction of Ia.
    J Exp Med. 1981 Jul 1;154(1):60-76 PMID: 7019381
  11. Increased secretion of plasminogen activator by human macrophages after exposure to leukocyte interferon.
    FEBS Lett. 1981 Jul 6;129(2):233-6 PMID: 6169547
  12. Regulation of human natural killing. I. The role of monocytes, interferon, and prostaglandins.
    J Immunol. 1981 Nov;127(5):2007-13 PMID: 6170683
  13. Evidence that types I and II interferons have different receptors.
    Nature. 1981 Dec 24;294(5843):768-70 PMID: 6172715
  14. Expression of human immune interferon cDNA in E. coli and monkey cells.
    Nature. 1982 Feb 11;295(5849):503-8 PMID: 6173769
  15. Interferon-induced changes in the monocyte membrane: inhibition by retinol and retinoic acid.
    Immunology. 1982 Mar;45(3):531-6 PMID: 6174424
  16. Expression of a mannosyl-fucosyl receptor for endocytosis on cultured primary macrophages and their hybrids.
    J Cell Biol. 1982 Apr;93(1):49-56 PMID: 6279673
  17. Down-regulation of mannosyl receptor-mediated endocytosis and antigen F4/80 in bacillus Calmette-Guérin-activated mouse macrophages. Role of T lymphocytes and lymphokines.
    J Exp Med. 1982 Jun 1;155(6):1623-37 PMID: 6978922
  18. Monoclonal antimacrophage antibodies: human pulmonary macrophages express HLA-DR (Ia-like) antigens in culture.
    Am Rev Respir Dis. 1982 May;125(5):586-93 PMID: 6952778
  19. Lymphokines enhance the capacity of human monocytes to secret reactive oxygen intermediates.
    J Clin Invest. 1982 Nov;70(5):1042-8 PMID: 7130393
  20. Interferon receptors. Cross-linking of human leukocyte interferon alpha-2 to its receptor on human cells.
    J Biol Chem. 1982 Dec 10;257(23):13884-7 PMID: 6292213
  21. Regulation of murine macrophage Ia antigen expression by a lymphokine with immune interferon activity.
    J Exp Med. 1982 Dec 1;156(6):1780-93 PMID: 6816896
  22. Analysis and purification of human lymphoblastoid (Namalwa) interferon using a monoclonal antibody.
    J Gen Virol. 1982 Nov;63 (Pt 1):207-12 PMID: 7175502
  23. Characterization of the mannose/fucose receptor on human mononuclear phagocytes.
    J Reticuloendothel Soc. 1982 Dec;32(6):423-31 PMID: 6298410
  24. Recombinant interferon-gamma increases HLA-DR synthesis and expression.
    J Immunol. 1983 Apr;130(4):1492-4 PMID: 6403609
  25. Macrophage-activating factor produced by a T cell hybridoma: physiochemical and biosynthetic resemblance to gamma-interferon.
    J Immunol. 1983 Aug;131(2):826-32 PMID: 6408190
  26. Superoxide release by peritoneal and bone marrow-derived mouse macrophages. Modulation by adherence and cell activation.
    Immunology. 1983 Aug;49(4):693-704 PMID: 6307867
  27. Identification of interferon-gamma as the lymphokine that activates human macrophage oxidative metabolism and antimicrobial activity.
    J Exp Med. 1983 Sep 1;158(3):670-89 PMID: 6411853
  28. Increased expression of human monocyte HLA-DR antigens and Fc gamma receptors in response to human interferon in vivo.
    Clin Exp Immunol. 1983 Sep;53(3):739-43 PMID: 6311468
  29. Immune interferon induces the receptor for monomeric IgG1 on human monocytic and myeloid cells.
    J Exp Med. 1983 Oct 1;158(4):1092-113 PMID: 6225822
  30. Lymphokines secreted by an established lymphocyte line modulate receptor-mediated endocytosis in macrophages derived from human monocytes.
    J Immunol. 1983 Nov;131(5):2368-73 PMID: 6313804
  31. Dexamethasone stimulates insulin receptor synthesis in cultured rat hepatocytes.
    J Biol Chem. 1983 Dec 10;258(23):14130-5 PMID: 6358216
  32. Activation of human monocyte cytotoxicity by natural and recombinant immune interferon.
    J Immunol. 1983 Dec;131(6):2821-6 PMID: 6417232
  33. Immune interferon and leukocyte-conditioned medium induce normal and leukemic myeloid cells to differentiate along the monocytic pathway.
    J Exp Med. 1983 Dec 1;158(6):2058-80 PMID: 6417261
  34. Glucocorticosteroid response modifying factors.
    Lymphokine Res. 1982;1(4):113-20 PMID: 6240574
  35. THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY.
    Biochem J. 1963 Oct;89:114-23 PMID: 14097352
  36. The interferons.
    Clin Exp Immunol. 1983 Oct;54(1):1-13 PMID: 6193915
  37. Esterases in human leukocytes.
    J Histochem Cytochem. 1973 Jan;21(1):1-12 PMID: 4694536
Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/JCI111740
Published
1985-02-00
Pages
624-31
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC423539
Subset
IM
External Links
PubMed source DOI: 10.1172/JCI111740 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2