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PMID: 2591367 Published · ppublish English Comparative Study Journal Article

Primary structure of sensory rhodopsin I, a prokaryotic photoreceptor.

The EMBO journal ·Vol. 8 ·No. 13 ·1989-12-20 ·Pages 3963-71

Blanck A, Oesterhelt D, Ferrando E, Schegk ES, Lottspeich F

Abstract

The gene coding for sensory rhodopsin I (SR-I) has been identified in a restriction fragment of genomic DNA from the Halobacterium halobium strain L33. Of the 1014 nucleotides whose sequence was determined, 720 belong to the structural gene of SR-I. In the 5' non-coding region two putative promoter elements and a ribosomal binding site have been identified. The 3' flanking region bears a potential terminator structure. The SR-I protein moiety carries no signal peptide and is not processed at its N terminus. The C terminus, however, lacks the last aspartic acid residue encoded by the gene. Analysis of the primary structure of SR-I reveals no consistent homology with the eukaryotic photoreceptor rhodopsin, but 14% homology with the halobacterial ion pumps, bacteriorhodopsin (BR) and halorhodopsin (HR). Residues conserved in all three proteins are discussed with respect to their contribution to secondary structure, retinal binding and ion translocation. The aspartic acid residue which mediates in BR the reprotonation of the Schiff base (D96) is replaced in SR-I by a tyrosine (Y87). This amino acid replacement is proposed to be of crucial importance in the evolution of the slow-cycling photosensing pigment SR-I.

MeSH Terms
Amino Acid Sequence Bacteriorhodopsins/genetics Base Sequence DNA, Bacterial/genetics,isolation & purification Genes, Bacterial Halobacterium/genetics Halorhodopsins Molecular Sequence Data Peptide Fragments/isolation & purification Protein Conformation Sensory Rhodopsins Sequence Homology, Nucleic Acid Terminator Regions, Genetic
Chemicals
DNA, Bacterial Halorhodopsins Peptide Fragments SRI protein, Halobacterium Sensory Rhodopsins Bacteriorhodopsins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Blanck A
Max-Planck-Institut für Biochemie, Martinsried, FRG.
Oesterhelt D
Ferrando E
Schegk E S
Lottspeich F
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1989-12-20
Pages
3963-71
Language
English
Region
England
NLM ID
8208664
PMCID
PMC401571
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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