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PMID: 2601696 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The rat albumin promoter is composed of six distinct positive elements within 130 nucleotides.

Molecular and cellular biology ·Vol. 9 ·No. 11 ·1989-11-00 ·Pages 4750-8

Herbomel P, Rollier A, Tronche F, Ott MO, Yaniv M, Weiss MC

Abstract

No fewer than six different positive regulatory elements concentrated within 130 base pairs constitute the rat albumin promoter, which drives highly tissue specific transcription in rat hepatoma cells in culture. Inactivation of each element led to a decrease in transcriptional efficiency: from upstream to downstream, 3- to 4-fold for distal elements III and II, 15-fold for distal element I, and 50-fold for the CCAAT box and the proximal element (PE). Three of these elements, distal elements III and II and, more crucially, the PE, were found to be involved in the tissue-specific character of transcription, with an additional negative regulation possibly superimposed at the level of the PE. Finally, our mapping of these regulatory elements in vivo entirely coincided with footprint data obtained in vitro, thereby allowing the tentative assignment of specific factors to the effects observed in vivo.

MeSH Terms
Albumins/genetics Animals Base Sequence Cells, Cultured DNA Mutational Analysis Liver/metabolism Molecular Sequence Data Mutation Organ Specificity/genetics Promoter Regions, Genetic Rats Regulatory Sequences, Nucleic Acid Transcription, Genetic
Chemicals
Albumins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Herbomel P
Unité des Virus Oncogènes, Institut Pasteur, Paris, France.
Rollier A
Tronche F
Ott M O
Yaniv M
Weiss M C
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.9.11.4750-4758.1989
Published
1989-11-00
Pages
4750-8
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC363623
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.9.11.4750-4758.1989 PMC Full Text View journal details
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