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PMID: 6096859 Published · ppublish English Journal Article

Mouse c-mos oncogene activation is prevented by upstream sequences.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 81 ·No. 24 ·1984-12-00 ·Pages 7817-21

Wood TG, McGeady ML, Baroudy BM, Blair DG, Vande Woude GF

Abstract

Although the molecularly cloned mouse c-mos oncogene locus can be efficiently activated by insertion of a retroviral long terminal repeat (LTR) 5' to its coding region, only low-frequency transformation occurs with the LTR element inserted 3' to this region. Analysis of several of the latter transformed cell lines suggested that loss of 2 kilobases (kb) of normal mouse DNA sequences preceding c-mos was required for oncogene activation. The determination of the transforming potential of deletion mutants containing only portions of this region followed by analysis of their nucleotide sequences identified a region termed upstream mouse sequence (UMS) as a cis-acting locus that prevents c-mos activation by a 3' LTR. The UMS region is approximately 1 kb in length and is located 0.8-1.8 kb upstream from the first ATG in the open reading frame of c-mos. Insertion of UMS 5' to the v-mos coding region also prevents 3' LTR enhancement of its transforming activity, but this inhibition is position dependent and functions only when inserted between v-mos and its putative promoter. The results presented here suggest that UMS may function to regulate c-mos proto-oncogene expression and may explain the lack of detectable c-mos transcripts in normal mouse cells.

MeSH Terms
Animals Base Sequence Cell Transformation, Neoplastic Cells, Cultured Cloning, Molecular DNA Restriction Enzymes Gene Expression Regulation Mice Mice, Inbred Strains Moloney murine sarcoma virus/genetics Oncogenes Transfection
Chemicals
DNA Restriction Enzymes
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Wood T G
McGeady M L
Baroudy B M
Blair D G
Vande Woude G F
References (42)
42 references, click to expand
  1. A virus-induced rhabdomyosarcoma of mice.
    Natl Cancer Inst Monogr. 1966 Sep;22:139-42 PMID: 4288557
  2. Are U4 small nuclear ribonucleoproteins involved in polyadenylation?
    Nature. 1984 May 10-16;309(5964):179-82 PMID: 6325940
  3. Sequence at the 3' end of globin mRNA shows homology with immunoglobulin light chain mRNA.
    Nature. 1974 Nov 29;252(5482):359-62 PMID: 4139664
  4. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  5. Methylmercury as a reversible denaturing agent for agarose gel electrophoresis.
    Anal Biochem. 1976 Jan;70(1):75-85 PMID: 1259158
  6. Nucleotide sequences in mouse DNA and RNA specific for Moloney sarcoma virus.
    Proc Natl Acad Sci U S A. 1976 Oct;73(10):3705-9 PMID: 185623
  7. Rate of divergence of cellular sequences homologous to segments of Moloney sarcoma virus.
    J Virol. 1977 Jan;21(1):153-60 PMID: 189062
  8. Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels.
    J Mol Biol. 1977 Feb 15;110(1):119-46 PMID: 845942
  9. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
    J Mol Biol. 1977 Jun 15;113(1):237-51 PMID: 881736
  10. Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5350-4 PMID: 414220
  11. Retroviruses.
    Annu Rev Biochem. 1978;47:35-88 PMID: 354499
  12. RNA structures near poly(A) of adenovirus-2 late messenger RNAs.
    J Mol Biol. 1978 Sep 5;124(1):27-31 PMID: 712833
  13. Properties of a normal mouse cell DNA sequence (sarc) homologous to the src sequence of Moloney sarcoma virus.
    Science. 1980 Mar 14;207(4436):1222-4 PMID: 6243788
  14. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  15. Computer analysis of nucleic acids and proteins.
    Methods Enzymol. 1980;65(1):595-609 PMID: 6154871
  16. Biological activity of cloned Moloney sarcoma virus DNA: Terminally redundant sequences may enhance transformation efficiency.
    Proc Natl Acad Sci U S A. 1980 Jun;77(6):3504-8 PMID: 6251463
  17. Nucleotide sequences of integrated Moloney sarcoma provirus long terminal repeats and their host and viral junctions.
    Proc Natl Acad Sci U S A. 1980 Jul;77(7):3937-41 PMID: 6254003
  18. Nucleotide sequence analysis of the transforming region and large terminal redundancies of Moloney murine sarcoma virus.
    Proc Natl Acad Sci U S A. 1980 Sep;77(9):5234-8 PMID: 6254069
  19. Transcripts from the adenovirus-2 major late promoter yield a single early family of 3' coterminal mRNAs and five late families.
    Cell. 1980 Dec;22(3):905-16 PMID: 7460018
  20. Enemies within: the genesis of retrovirus oncogenes.
    Cell. 1981 Jan;23(1):5-6 PMID: 7214527
  21. Systematic punctuation of eukaryotic DNA by A+T-rich sequences.
    Proc Natl Acad Sci U S A. 1981 Mar;78(3):1341-5 PMID: 6940161
  22. Activation of the transforming potential of a normal cell sequence: a molecular model for oncogenesis.
    Science. 1981 May 22;212(4497):941-3 PMID: 7233190
  23. The sequence 5'-AAUAAA-3'forms parts of the recognition site for polyadenylation of late SV40 mRNAs.
    Cell. 1981 Apr;24(1):251-60 PMID: 6113054
  24. Alpha-Globin-gene switching during the development of chicken embryos: expression and chromosome structure.
    Cell. 1981 May;24(2):333-44 PMID: 7237551
  25. Transcription units of adenovirus type 2. Termination of transcription beyond the poly(A) addition site in early regions 2 and 4.
    J Mol Biol. 1980 Dec 15;144(3):377-86 PMID: 7253021
  26. The long terminal repeat of Moloney sarcoma provirus.
    Cold Spring Harb Symp Quant Biol. 1981;45 Pt 2:699-705 PMID: 6266761
  27. Nucleotide sequence of Moloney murine leukaemia virus.
    Nature. 1981 Oct 15-21;293(5833):543-8 PMID: 6169994
  28. Nucleotide sequence of the genome of a murine sarcoma virus.
    Cell. 1981 Nov;27(1 Pt 2):97-108 PMID: 6173134
  29. A + T-rich linkers define functional domains in eukaryotic DNA.
    Nature. 1982 Jan 21;295(5846):260-2 PMID: 6460190
  30. Activation of SV40 genome by 72-base pair tandem repeats of Moloney sarcoma virus.
    Nature. 1982 Feb 18;295(5850):568-72 PMID: 6276775
  31. Cellular Moloney murine sarcoma (c-mos) sequences are hypermethylated and transcriptionally silent in normal and transformed rodent cells.
    Mol Cell Biol. 1982 Jan;2(1):42-51 PMID: 6287220
  32. Human DNA sequence homologous to the transforming gene (mos) of Moloney murine sarcoma virus.
    Proc Natl Acad Sci U S A. 1982 Jul;79(13):4078-82 PMID: 6287464
  33. Activation of a cellular oncogene by DNA rearrangement: possible involvement of an IS-like element.
    Nature. 1982 Dec 16;300(5893):607-11 PMID: 6292737
  34. Globin RNA transcription: a possible termination site and demonstration of transcriptional control correlated with altered chromatin structure.
    Cell. 1982 Jul;29(3):887-93 PMID: 7151174
  35. Recombinational junctions of variants of Moloney murine sarcoma virus: generation and divergence of a mammalian transforming gene.
    J Virol. 1983 Feb;45(2):607-17 PMID: 6300424
  36. Homology between an endogenous viral LTR and sequences inserted in an activated cellular oncogene.
    Nature. 1983 Apr 7;302(5908):547-8 PMID: 6835385
  37. Somatic generation of antibody diversity.
    Nature. 1983 Apr 14;302(5909):575-81 PMID: 6300689
  38. Long terminal repeat enhancement of v-mos transforming activity: identification of essential regions.
    J Virol. 1983 Jun;46(3):726-36 PMID: 6190012
  39. Cellular oncogenes and retroviruses.
    Annu Rev Biochem. 1983;52:301-54 PMID: 6351725
  40. Rearranged c-mos locus in a MOPC 21 murine myeloma cell line and its persistence in hybridomas.
    Nature. 1983 Dec 22-1984 Jan 4;306(5945):795-6 PMID: 6656882
  41. Rearrangement of the oncogene c-mos in mouse myeloma NSI and hybridomas.
    Nature. 1983 Dec 22-1984 Jan 4;306(5945):797-9 PMID: 6656883
  42. A new technique for the assay of infectivity of human adenovirus 5 DNA.
    Virology. 1973 Apr;52(2):456-67 PMID: 4705382
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.81.24.7817
Published
1984-12-00
Pages
7817-21
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC392243
Subset
IM
Databases
GENBANK
J00371, J00372
External Links
PubMed source DOI: 10.1073/pnas.81.24.7817 PMC Full Text View journal details
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