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PMID: 26300860 Published · epublish English Journal Article Review

MALDI-TOF mass spectrometry: an emerging technology for microbial identification and diagnosis.

Frontiers in microbiology ·Vol. 6 ·2015-00-00 ·Pages 791

Singhal N, Kumar M, Kanaujia PK, Virdi JS

Abstract

Currently microorganisms are best identified using 16S rRNA and 18S rRNA gene sequencing. However, in recent years matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has emerged as a potential tool for microbial identification and diagnosis. During the MALDI-TOF MS process, microbes are identified using either intact cells or cell extracts. The process is rapid, sensitive, and economical in terms of both labor and costs involved. The technology has been readily imbibed by microbiologists who have reported usage of MALDI-TOF MS for a number of purposes like, microbial identification and strain typing, epidemiological studies, detection of biological warfare agents, detection of water- and food-borne pathogens, detection of antibiotic resistance and detection of blood and urinary tract pathogens etc. The limitation of the technology is that identification of new isolates is possible only if the spectral database contains peptide mass fingerprints of the type strains of specific genera/species/subspecies/strains. This review provides an overview of the status and recent applications of mass spectrometry for microbial identification. It also explores the usefulness of this exciting new technology for diagnosis of diseases caused by bacteria, viruses, and fungi.

Keywords
MALDI-TOF MS bacterial identification fungi peptide mass fingerprint proteomics viruses
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Singhal Neelja
Department of Microbiology, University of Delhi New Delhi, India.
Kumar Manish
Department of Biophysics, University of Delhi New Delhi, India.
Kanaujia Pawan K
Department of Microbiology, University of Delhi New Delhi, India.
Virdi Jugsharan S
Department of Microbiology, University of Delhi New Delhi, India.
References (193)
193 references, click to expand
  1. Reliable identification at the species level of Brucella isolates with MALDI-TOF-MS.
    BMC Microbiol. 2011 Dec 23;11:267 PMID: 22192890
  2. Evaluation of matrix-assisted laser desorption ionization-time of flight mass spectrometry for identification of clinically important yeast species.
    J Clin Microbiol. 2010 Oct;48(10):3482-6 PMID: 20668126
  3. MALDI-TOF MS for the diagnosis of infectious diseases.
    Clin Chem. 2015 Jan;61(1):100-11 PMID: 25278500
  4. MALDI-TOF mass spectrometry is a fast and reliable platform for identification and ecological studies of species from family Rhizobiaceae.
    PLoS One. 2011;6(5):e20223 PMID: 21655291
  5. Discrimination of Aspergillus isolates at the species and strain level by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry fingerprinting.
    Anal Biochem. 2008 Sep 15;380(2):276-81 PMID: 18577370
  6. Typing of Ochrobactrum anthropi clinical isolates using automated repetitive extragenic palindromic-polymerase chain reaction DNA fingerprinting and matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry.
    BMC Microbiol. 2014 Mar 22;14:74 PMID: 24655432
  7. Rapid discrimination between strains of beta haemolytic streptococci by intact cell mass spectrometry.
    Indian J Med Res. 2004 Jun;119(6):283-8 PMID: 15243166
  8. Direct application of MALDI-TOF mass spectrometry to cerebrospinal fluid for rapid pathogen identification in a patient with bacterial meningitis.
    Clin Chim Acta. 2014 Aug 5;435:59-61 PMID: 24797349
  9. Use of matrix-assisted laser desorption ionization-time of flight mass spectrometry to identify vancomycin-resistant enterococci and investigate the epidemiology of an outbreak.
    J Clin Microbiol. 2012 Sep;50(9):2918-31 PMID: 22740710
  10. High-throughput identification of bacteria and yeast by matrix-assisted laser desorption ionization-time of flight mass spectrometry in conventional medical microbiology laboratories.
    J Clin Microbiol. 2010 Mar;48(3):900-7 PMID: 20053859
  11. Matrix-assisted laser desorption ionization-time of flight mass spectrometry as an alternative to 16S rRNA gene sequencing for identification of difficult-to-identify bacterial strains.
    J Clin Microbiol. 2011 Feb;49(2):693-6 PMID: 21106794
  12. Investigation of spectral reproducibility in direct analysis of bacteria proteins by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
    Rapid Commun Mass Spectrom. 1998;12(8):456-64 PMID: 9586233
  13. Detection of carbapenemase activities of Bacteroides fragilis strains with matrix-assisted laser desorption ionization--time of flight mass spectrometry (MALDI-TOF MS).
    Anaerobe. 2014 Apr;26:49-52 PMID: 24480431
  14. GyrB sequence analysis and MALDI-TOF MS as identification tools for plant pathogenic Clavibacter.
    Syst Appl Microbiol. 2011 Sep;34(6):400-7 PMID: 21802235
  15. Application of matrix-assisted laser desorption ionization time-of-flight mass spectrometry in the screening of vanA-positive Enterococcus faecium.
    Eur J Mass Spectrom (Chichester). 2014;20(6):461-5 PMID: 25905870
  16. Identification of dermatophyte species causing onychomycosis and tinea pedis by MALDI-TOF mass spectrometry.
    Exp Dermatol. 2008 Apr;17(4):356-61 PMID: 17979969
  17. Simultaneous detection and identification of enteric viruses by PCR-mass assay.
    PLoS One. 2012;7(8):e42251 PMID: 22870310
  18. Evaluation of MALDI-TOF mass spectrometry for the identification of medically-important yeasts in the clinical laboratories of Dijon and Lille hospitals.
    Med Mycol. 2013 Jan;51(1):25-32 PMID: 22703164
  19. Sensitive and rapid detection of viruses associated with hand foot and mouth disease using multiplexed MALDI-TOF analysis.
    J Clin Virol. 2013 Feb;56(2):170-4 PMID: 23194776
  20. Evolution of Streptococcus pneumoniae and its close commensal relatives.
    PLoS One. 2008 Jul 16;3(7):e2683 PMID: 18628950
  21. Species differentiation of seafood spoilage and pathogenic gram-negative bacteria by MALDI-TOF mass fingerprinting.
    J Proteome Res. 2010 Jun 4;9(6):3169-83 PMID: 20408567
  22. MALDI-TOF mass spectrometry of bacteria.
    Mass Spectrom Rev. 2001 Jul-Aug;20(4):172-94 PMID: 11835305
  23. Direct identification of bacteria in urine samples by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and relevance of defensins as interfering factors.
    J Med Microbiol. 2012 Mar;61(Pt 3):339-44 PMID: 22275503
  24. Rapid and accurate identification of genomic species from the Acinetobacter baumannii (Ab) group by MALDI-TOF MS.
    Clin Microbiol Infect. 2012 Nov;18(11):1097-103 PMID: 22085042
  25. Performance of matrix-assisted laser desorption ionization-time of flight mass spectrometry for identification of bacterial strains routinely isolated in a clinical microbiology laboratory.
    J Clin Microbiol. 2010 May;48(5):1549-54 PMID: 20220166
  26. MALDI-TOF-MS-based species identification and typing approaches in medical mycology.
    Proteomics. 2013 Mar;13(5):788-99 PMID: 23281257
  27. Robustness of two MALDI-TOF mass spectrometry systems for bacterial identification.
    J Microbiol Methods. 2012 May;89(2):133-6 PMID: 22425492
  28. Rapid identification of microorganisms from positive blood cultures by MALDI-TOF mass spectrometry subsequent to very short-term incubation on solid medium.
    Clin Microbiol Infect. 2014 Oct;20(10):1001-6 PMID: 24698361
  29. Can MALDI-TOF mass spectrometry be used with intravascular catheters?
    Enferm Infecc Microbiol Clin. 2014 Jun-Jul;32(6):372-4 PMID: 24679820
  30. Rapid identification of Cryptococcus neoformans and Cryptococcus gattii by matrix-assisted laser desorption ionization-time of flight mass spectrometry.
    J Clin Microbiol. 2011 Aug;49(8):3050-3 PMID: 21653762
  31. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry identification of mycobacteria in routine clinical practice.
    PLoS One. 2011;6(9):e24720 PMID: 21935444
  32. pkDACLASS: Open source software for analyzing MALDI-TOF data.
    Bioinformation. 2011 Mar 02;6(1):45-7 PMID: 21464846
  33. Rapid identification of environmental bacterial strains by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
    Rapid Commun Mass Spectrom. 2004;18(18):2013-9 PMID: 15378711
  34. Characterization of intact microorganisms by MALDI mass spectrometry.
    Mass Spectrom Rev. 2001 Jul-Aug;20(4):157-71 PMID: 11835304
  35. Differentiation of Aeromonas isolated from drinking water distribution systems using matrix-assisted laser desorption/ionization-mass spectrometry.
    Anal Chem. 2007 Mar 1;79(5):1939-46 PMID: 17269751
  36. Direct bacterial profiling by matrix-assisted laser desorption-ionization time-of-flight mass spectrometry for identification of pathogenic Neisseria.
    J Mol Diagn. 2009 Jan;11(1):75-86 PMID: 19095774
  37. Comparison of direct colony method versus extraction method for identification of gram-positive cocci by use of Bruker Biotyper matrix-assisted laser desorption ionization-time of flight mass spectrometry.
    J Clin Microbiol. 2011 Aug;49(8):2868-73 PMID: 21613431
  38. Ongoing revolution in bacteriology: routine identification of bacteria by matrix-assisted laser desorption ionization time-of-flight mass spectrometry.
    Clin Infect Dis. 2009 Aug 15;49(4):543-51 PMID: 19583519
  39. Polyphasic analysis of strains of the genus Capnocytophaga and Centers for Disease Control group DF-3.
    Int J Syst Bacteriol. 1996 Jul;46(3):782-91 PMID: 8782690
  40. Origins of the reassortant 2009 pandemic influenza virus through proteotyping with mass spectrometry.
    J Mass Spectrom. 2014 Jan;49(1):93-102 PMID: 24446268
  41. Sensitive and rapid detection of ganciclovir resistance by PCR based MALDI-TOF analysis.
    J Clin Virol. 2012 Aug;54(4):359-63 PMID: 22633201
  42. Prokaryotic diversity--magnitude, dynamics, and controlling factors.
    Science. 2002 May 10;296(5570):1064-6 PMID: 12004116
  43. Rapid screening of epidemiologically important Salmonella enterica subsp. enterica serovars by whole-cell matrix-assisted laser desorption ionization-time of flight mass spectrometry.
    Appl Environ Microbiol. 2011 Jun;77(12):4136-46 PMID: 21515723
  44. Differential characterization of biogenic amine-producing bacteria involved in food poisoning using MALDI-TOF mass fingerprinting.
    Electrophoresis. 2010 Mar;31(6):1116-27 PMID: 20151397
  45. Rapid identification of Staphylococci isolated in clinical microbiology laboratories by matrix-assisted laser desorption ionization-time of flight mass spectrometry.
    J Clin Microbiol. 2007 Jul;45(7):2156-61 PMID: 17507519
  46. Identification of lethal Aspergillus at early growth stages based on matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
    Diagn Microbiol Infect Dis. 2011 Jul;70(3):344-54 PMID: 21546196
  47. Robust hepatitis B virus genotyping by mass spectrometry.
    J Clin Microbiol. 2010 Nov;48(11):4161-8 PMID: 20810764
  48. Identification of biomarkers of whole Coxiella burnetii phase I by MALDI-TOF mass spectrometry.
    Anal Chem. 2004 Jul 15;76(14):4017-22 PMID: 15253637
  49. Matrix-assisted laser desorption and ionization-time-of-flight mass spectrometry, 16S rRNA gene sequencing, and API 32E for identification of Cronobacter spp.: a comparative study.
    J Food Prot. 2011 Dec;74(12):2182-7 PMID: 22186062
  50. Subtyping of the influenza virus by high resolution mass spectrometry.
    Anal Chem. 2009 May 1;81(9):3500-6 PMID: 19402721
  51. SpectraBank: an open access tool for rapid microbial identification by MALDI-TOF MS fingerprinting.
    Electrophoresis. 2012 Jul;33(14):2138-42 PMID: 22821489
  52. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry for fast and accurate identification of clinically relevant Aspergillus species.
    Clin Microbiol Infect. 2011 May;17(5):750-5 PMID: 20673266
  53. Rapid screening and dereplication of bacterial isolates from marine sponges of the sula ridge by intact-cell-MALDI-TOF mass spectrometry (ICM-MS).
    Appl Microbiol Biotechnol. 2005 Jun;67(4):539-48 PMID: 15614563
  54. Rapid genus- and species-specific identification of Cronobacter spp. by matrix-assisted laser desorption ionization-time of flight mass spectrometry.
    J Clin Microbiol. 2010 Aug;48(8):2846-51 PMID: 20554814
  55. Identification of Francisella tularensis by whole-cell matrix-assisted laser desorption ionization-time of flight mass spectrometry: fast, reliable, robust, and cost-effective differentiation on species and subspecies levels.
    J Clin Microbiol. 2010 Apr;48(4):1061-9 PMID: 20181907
  56. Strain and phase identification of the U.S. category B agent Coxiella burnetii by matrix assisted laser desorption/ionization time-of-flight mass spectrometry and multivariate pattern recognition.
    Anal Chim Acta. 2007 Jan 30;583(1):23-31 PMID: 17386522
  57. Quantitative proteomics to study carbapenem resistance in Acinetobacter baumannii.
    Front Microbiol. 2014 Sep 26;5:512 PMID: 25309531
  58. The development of a matrix-assisted laser desorption/ionization mass spectrometry-based method for the protein fingerprinting and identification of Aeromonas species using whole cells.
    J Microbiol Methods. 2006 Jun;65(3):380-9 PMID: 16176841
  59. Effects of varied pH, growth rate and temperature using controlled fermentation and batch culture on matrix assisted laser desorption/ionization whole cell protein fingerprints.
    J Microbiol Methods. 2005 Sep;62(3):259-71 PMID: 15979749
  60. A description of the lactic acid bacteria microbiota associated with the production of traditional fermented vegetables in Vietnam.
    Int J Food Microbiol. 2013 Apr 15;163(1):19-27 PMID: 23500611
  61. Identification of Acinetobacter species: is Bruker biotyper MALDI-TOF mass spectrometry a good alternative to molecular techniques?
    Infect Genet Evol. 2012 Mar;12(2):345-9 PMID: 22266021
  62. Short communication: Identification of subclinical cow mastitis pathogens in milk by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
    J Dairy Sci. 2010 Dec;93(12):5661-7 PMID: 21094737
  63. Identification of European mosquito species by MALDI-TOF MS.
    Parasitol Res. 2014 Jun;113(6):2375-8 PMID: 24737398
  64. Evaluation of the MALDI TOF-MS method for identification of Candida strains isolated from blood cultures.
    Diagn Microbiol Infect Dis. 2012 May;73(1):65-7 PMID: 22578939
  65. Discrimination of Francisella tularensis subspecies using surface enhanced laser desorption ionization mass spectrometry and multivariate data analysis.
    FEMS Microbiol Lett. 2005 Feb 1;243(1):303-10 PMID: 15668033
  66. Rapid discrimination of environmental Vibrio by matrix-assisted laser desorption ionization time-of-flight mass spectrometry.
    Microbiol Res. 2012 Apr 20;167(4):226-30 PMID: 22015259
  67. Matrix-assisted laser desorption ionization-time of flight (mass spectrometry) for hepatitis C virus genotyping.
    J Clin Microbiol. 2005 Jun;43(6):2810-5 PMID: 15956402
  68. Identification of haemolytic Haemophilus species isolated from human clinical specimens and description of Haemophilus sputorum sp. nov.
    Int J Med Microbiol. 2012 Mar;302(2):78-83 PMID: 22336150
  69. Identification of harmless and pathogenic algae of the genus Prototheca by MALDI-MS.
    Proteomics Clin Appl. 2009 Jul;3(7):774-84 PMID: 21136986
  70. The essence on mass spectrometry based microbial diagnostics.
    Curr Opin Microbiol. 2012 Jun;15(3):397-402 PMID: 22410108
  71. Characterisation of bacteria by matrix-assisted laser desorption/ionisation and electrospray mass spectrometry.
    FEMS Microbiol Rev. 2000 Apr;24(2):193-219 PMID: 10717314
  72. Performance of the VITEK MS matrix-assisted laser desorption ionization-time of flight mass spectrometry system for rapid bacterial identification in two diagnostic centres in China.
    J Med Microbiol. 2015 Jan;64(Pt 1):18-24 PMID: 25418737
  73. Rapid identification and speciation of Haemophilus bacteria by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
    J Mass Spectrom. 1998 Aug;33(8):750-6 PMID: 9745723
  74. Rapid identification and typing of Yersinia pestis and other Yersinia species by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry.
    BMC Microbiol. 2010 Nov 12;10:285 PMID: 21073689
  75. Rapid and accurate bacterial identification in probiotics and yoghurts by MALDI-TOF mass spectrometry.
    J Food Sci. 2011 Oct;76(8):M568-72 PMID: 22417598
  76. Matrix-assisted laser desorption ionization-time of flight mass spectrometry-based functional assay for rapid detection of resistance against β-lactam antibiotics.
    J Clin Microbiol. 2012 Mar;50(3):927-37 PMID: 22205812
  77. Detection and quantification of bacterial spoilage in milk and pork meat using MALDI-TOF-MS and multivariate analysis.
    Anal Chem. 2012 Jul 17;84(14):5951-8 PMID: 22698768
  78. Evaluation of matrix-assisted laser desorption ionization-time of flight mass spectrometry for rapid identification of Beta-hemolytic streptococci.
    J Clin Microbiol. 2011 Aug;49(8):3004-5 PMID: 21697322
  79. Rapid detection of Salmonella sp. by means of a combination of selective enrichment broth and MALDI-TOF MS.
    Eur J Clin Microbiol Infect Dis. 2012 May;31(5):767-73 PMID: 21842294
  80. Applications of MALDI-TOF mass spectrometry in clinical diagnostic microbiology.
    FEMS Microbiol Rev. 2012 Mar;36(2):380-407 PMID: 22092265
  81. Development of a MALDI two-layer volume sample preparation technique for analysis of colored conidia spores of Fusarium by MALDI linear TOF mass spectrometry.
    Anal Bioanal Chem. 2009 Nov;395(5):1373-83 PMID: 19727682
  82. Formic acid-based direct, on-plate testing of yeast and Corynebacterium species by Bruker Biotyper matrix-assisted laser desorption ionization-time of flight mass spectrometry.
    J Clin Microbiol. 2012 Sep;50(9):3093-5 PMID: 22760034
  83. Rapid and specific influenza virus detection by functionalized magnetic nanoparticles and mass spectrometry.
    J Nanobiotechnology. 2011 Nov 16;9:52 PMID: 22088100
  84. Rapid species identification of seafood spoilage and pathogenic Gram-positive bacteria by MALDI-TOF mass fingerprinting.
    Electrophoresis. 2011 Nov;32(21):2951-65 PMID: 22009363
  85. Rapid identification of protein biomarkers of Escherichia coli O157:H7 by matrix-assisted laser desorption ionization-time-of-flight-time-of-flight mass spectrometry and top-down proteomics.
    Anal Chem. 2010 Apr 1;82(7):2717-25 PMID: 20232878
  86. Liquid chromatography/mass spectrometry characterization of Escherichia coli and Shigella species.
    J Am Soc Mass Spectrom. 2008 Nov;19(11):1621-8 PMID: 18692404
  87. Application of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for the study of Helicobacter pylori.
    Rapid Commun Mass Spectrom. 2010 Feb;24(3):328-34 PMID: 20049887
  88. Identification of Aeromonas isolates by matrix-assisted laser desorption ionization time-of-flight mass spectrometry.
    Diagn Microbiol Infect Dis. 2011 Sep;71(1):1-5 PMID: 21763094
  89. MALDI-TOF mass spectrometry - a rapid method for the identification of dermatophyte species.
    Med Mycol. 2013 Jan;51(1):17-24 PMID: 22574631
  90. MALDI-TOF mass spectrometry compatible inactivation method for highly pathogenic microbial cells and spores.
    Anal Chem. 2008 Mar 15;80(6):2026-34 PMID: 18290666
  91. Multiplex detection of protein toxins using MALDI-TOF-TOF tandem mass spectrometry: application in unambiguous toxin detection from bioaerosol.
    Anal Chem. 2012 Dec 4;84(23):10500-7 PMID: 23083074
  92. Diafiltration MALDI-TOF mass spectrometry method for culture-independent detection and identification of pathogens directly from urine specimens.
    Am J Clin Pathol. 2014 Feb;141(2):204-12 PMID: 24436267
  93. Rapid identification and classification of Mycobacterium spp. using whole-cell protein barcodes with matrix assisted laser desorption ionization time of flight mass spectrometry in comparison with multigene phylogenetic analysis.
    Anal Chim Acta. 2012 Feb 24;716:133-7 PMID: 22284888
  94. Typing of nosocomial outbreaks of Acinetobacter baumannii by use of matrix-assisted laser desorption ionization-time of flight mass spectrometry.
    J Clin Microbiol. 2013 Feb;51(2):603-6 PMID: 23175257
  95. Identification of non-diphtheriae corynebacterium by use of matrix-assisted laser desorption ionization-time of flight mass spectrometry.
    J Clin Microbiol. 2012 Jan;50(1):160-3 PMID: 22075579
  96. Characterization of intact Penicillium spores by matrix-assisted laser desorption/ionization mass spectrometry.
    Rapid Commun Mass Spectrom. 2005;19(23):3564-8 PMID: 16276495
  97. Multiplex detection of 60 hepatitis B virus variants by maldi-tof mass spectrometry.
    Clin Chem. 2009 Aug;55(8):1503-9 PMID: 19541863
  98. Differentiation of vanA-positive Enterococcus faecium from vanA-negative E. faecium by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry.
    Int J Antimicrob Agents. 2014 Sep;44(3):256-9 PMID: 25104134
  99. Fingerprinting of Helicobacter pylori strains by matrix-assisted laser desorption/ionization mass spectrometric analysis.
    Rapid Commun Mass Spectrom. 1999;13(11):1067-71 PMID: 10368980
  100. Localization of analyte molecules in MALDI preparations by confocal laser scanning microscopy.
    Anal Chem. 2001 Mar 1;73(5):1016-22 PMID: 11289411
  101. Characterization of β-lactamase enzyme activity in bacterial lysates using MALDI-mass spectrometry.
    J Proteome Res. 2012 Jan 1;11(1):79-84 PMID: 22013912
  102. A novel approach to identify plant parasitic nematodes using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
    Rapid Commun Mass Spectrom. 2005;19(11):1454-60 PMID: 15880621
  103. Biotyping of multidrug-resistant Klebsiella pneumoniae clinical isolates from France and Algeria using MALDI-TOF MS.
    PLoS One. 2013 Apr 19;8(4):e61428 PMID: 23620754
  104. Evaluation of matrix-assisted laser desorption ionization-time of flight whole cell profiles for assessing the cultivable diversity of aerobic and moderately halophilic prokaryotes thriving in solar saltern sediments.
    Syst Appl Microbiol. 2011 Feb;34(1):69-75 PMID: 21242046
  105. Antifungal activities of posaconazole, ravuconazole, and voriconazole compared to those of itraconazole and amphotericin B against 239 clinical isolates of Aspergillus spp. and other filamentous fungi: report from SENTRY Antimicrobial Surveillance Program, 2000.
    Antimicrob Agents Chemother. 2002 Apr;46(4):1032-7 PMID: 11897586
  106. A molecular view of microbial diversity and the biosphere.
    Science. 1997 May 2;276(5313):734-40 PMID: 9115194
  107. Identification of a variety of Staphylococcus species by matrix-assisted laser desorption ionization-time of flight mass spectrometry.
    J Clin Microbiol. 2010 Mar;48(3):941-5 PMID: 20032251
  108. Evaluation of matrix-assisted laser desorption/ionization (MALDI) preparation techniques for surface characterization of intact Fusarium spores by MALDI linear time-of-flight mass spectrometry.
    Rapid Commun Mass Spectrom. 2009 Mar;23(6):877-84 PMID: 19224532
  109. Rapid identification of bacteria in positive blood culture broths by matrix-assisted laser desorption ionization-time of flight mass spectrometry.
    J Clin Microbiol. 2010 Feb;48(2):444-7 PMID: 19955282
  110. Intact cell mass spectrometry (ICMS) used to type methicillin-resistant Staphylococcus aureus: media effects and inter-laboratory reproducibility.
    J Microbiol Methods. 2002 Feb;48(2-3):117-26 PMID: 11777562
  111. Direct maldi-tof mass spectrometry assay of blood culture broths for rapid identification of Candida species causing bloodstream infections: an observational study in two large microbiology laboratories.
    J Clin Microbiol. 2012 Jan;50(1):176-9 PMID: 22090401
  112. Characterization of protein biomarkers desorbed by MALDI from whole fungal cells.
    Anal Chem. 2001 Nov 1;73(21):5228-31 PMID: 11721923
  113. Matrix-assisted laser desorption ionization-time of flight mass spectrometry for direct bacterial identification from positive blood culture pellets.
    J Clin Microbiol. 2010 Apr;48(4):1481-3 PMID: 20164269
  114. Matrix-assisted laser desorption ionization-time of flight mass spectrometry for the identification of clinically relevant bacteria.
    PLoS One. 2011 Jan 25;6(1):e16424 PMID: 21283545
  115. The use of ITS DNA sequence analysis and MALDI-TOF mass spectrometry in diagnosing an infection with Fusarium proliferatum.
    Exp Dermatol. 2008 Nov;17(11):965-71 PMID: 18547323
  116. What is new in clinical microbiology-microbial identification by MALDI-TOF mass spectrometry: a paper from the 2011 William Beaumont Hospital Symposium on molecular pathology.
    J Mol Diagn. 2012 Sep;14(5):419-23 PMID: 22795961
  117. [Study on detection and identification of Salmonella species by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry].
    Wei Sheng Yan Jiu. 2008 Nov;37(6):685-9 PMID: 19239002
  118. Direct analysis and identification of pathogenic Lichtheimia species by matrix-assisted laser desorption ionization-time of flight analyzer-mediated mass spectrometry.
    J Clin Microbiol. 2012 Feb;50(2):419-27 PMID: 22135259
  119. Identification of Streptococcus agalactiae isolates from various phylogenetic lineages by matrix-assisted laser desorption ionization-time of flight mass spectrometry.
    J Clin Microbiol. 2009 Jul;47(7):2284-7 PMID: 19403759
  120. Identification of mycobacteria in solid-culture media by matrix-assisted laser desorption ionization-time of flight mass spectrometry.
    J Clin Microbiol. 2011 May;49(5):1790-4 PMID: 21411597
  121. Integrated microanalytical technology enabling rapid and automated protein identification.
    Anal Chem. 2000 Jan 15;72(2):286-93 PMID: 10658321
  122. Discrimination of different species from the genus Drosophila by intact protein profiling using matrix-assisted laser desorption ionization mass spectrometry.
    BMC Evol Biol. 2010 Apr 07;10:95 PMID: 20374617
  123. Evaluation of matrix-assisted laser desorption ionization-time-of-flight mass spectrometry in comparison to 16S rRNA gene sequencing for species identification of nonfermenting bacteria.
    J Clin Microbiol. 2008 Jun;46(6):1946-54 PMID: 18400920
  124. MALDI-TOF mass spectrometry and microsatellite markers to evaluate Candida parapsilosis transmission in neonatal intensive care units.
    Eur J Clin Microbiol Infect Dis. 2012 Nov;31(11):2919-28 PMID: 22644055
  125. Comparison of matrix-assisted laser desorption ionization-time of flight (maldi-tof) mass spectrometry platforms for the identification of gram-negative rods from patients with cystic fibrosis.
    J Clin Microbiol. 2013 Nov;51(11):3852-4 PMID: 23966494
  126. Identification of Tsetse (Glossina spp.) using matrix-assisted laser desorption/ionisation time of flight mass spectrometry.
    PLoS Negl Trop Dis. 2013 Jul 11;7(7):e2305 PMID: 23875040
  127. MALDI-TOF MS, a useful instrument for differentiating metallo-β-lactamases in Enterobacteriaceae and Pseudomonas spp.
    Lett Appl Microbiol. 2014 Apr;58(4):325-9 PMID: 24286119
  128. Rapid species specific identification and subtyping of Yersinia enterocolitica by MALDI-TOF mass spectrometry.
    J Microbiol Methods. 2011 Nov;87(2):150-3 PMID: 21914454
  129. MALDI-TOF MS: an upcoming tool for rapid detection of antibiotic resistance in microorganisms.
    Proteomics Clin Appl. 2013 Dec;7(11-12):767-78 PMID: 24123965
  130. Rapid identification of Vibrio parahaemolyticus by whole-cell matrix-assisted laser desorption ionization-time of flight mass spectrometry.
    Appl Environ Microbiol. 2009 Nov;75(21):6745-56 PMID: 19749061
  131. Antifungal susceptibility profile of human-pathogenic species of Lichtheimia.
    Antimicrob Agents Chemother. 2010 Jul;54(7):3058-60 PMID: 20421405
  132. A method for the detection of antibiotic resistance markers in clinical strains of Escherichia coli using MALDI mass spectrometry.
    J Microbiol Methods. 2015 Apr;111:1-8 PMID: 25633625
  133. Identification of Leishmania at the species level with matrix-assisted laser desorption ionization time-of-flight mass spectrometry.
    Clin Microbiol Infect. 2014 Jun;20(6):551-7 PMID: 24165542
  134. A new PCR-based mass spectrometry system for high-risk HPV, part I: methods.
    Am J Clin Pathol. 2011 Dec;136(6):913-9 PMID: 22095377
  135. Development of a clinically comprehensive database and a simple procedure for identification of molds from solid media by matrix-assisted laser desorption ionization-time of flight mass spectrometry.
    J Clin Microbiol. 2013 Mar;51(3):828-34 PMID: 23269728
  136. MALDI-TOF mass signatures for differentiation of yeast species, strain grouping and monitoring of morphogenesis markers.
    Anal Bioanal Chem. 2008 Oct;392(3):439-49 PMID: 18690424
  137. Carbapenemase activity detection by matrix-assisted laser desorption ionization-time of flight mass spectrometry.
    J Clin Microbiol. 2011 Sep;49(9):3222-7 PMID: 21775535
  138. Rapid identification of bacteria from positive blood culture bottles by use of matrix-assisted laser desorption-ionization time of flight mass spectrometry fingerprinting.
    J Clin Microbiol. 2010 May;48(5):1584-91 PMID: 20237093
  139. New procedure for rapid identification of microorganisms causing urinary tract infection from urine samples by mass spectrometry (MALDI-TOF).
    Enferm Infecc Microbiol Clin. 2015 Feb;33(2):89-94 PMID: 24796945
  140. Rapid identification of viridans streptococci by mass spectrometric discrimination.
    J Clin Microbiol. 2007 Aug;45(8):2392-7 PMID: 17553974
  141. Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry applied to virus identification.
    Sci Rep. 2014 Oct 30;4:6803 PMID: 25354905
  142. MALDI-TOF MS fingerprinting allows for discrimination of major methicillin-resistant Staphylococcus aureus lineages.
    Int J Med Microbiol. 2011 Jan;301(1):64-8 PMID: 20728405
  143. Mass spectrometry biotyper system identifies enteric bacterial pathogens directly from colonies grown on selective stool culture media.
    J Clin Microbiol. 2010 Nov;48(11):3888-92 PMID: 20844226
  144. Species identification of clinical isolates of Bacteroides by matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry.
    Clin Microbiol Infect. 2009 Aug;15(8):796-802 PMID: 19438622
  145. Novel mass spectrometry-based tool for genotypic identification of mycobacteria.
    J Clin Microbiol. 2004 Jan;42(1):339-46 PMID: 14715774
  146. Direct identification of urinary tract pathogens from urine samples by matrix-assisted laser desorption ionization-time of flight mass spectrometry.
    J Clin Microbiol. 2010 Jun;48(6):2110-5 PMID: 20392910
  147. Rapid Identification of microbes in positive blood cultures by use of the vitek MS matrix-assisted laser desorption ionization-time of flight mass spectrometry system.
    J Clin Microbiol. 2013 Nov;51(11):3717-9 PMID: 23985920
  148. Identification of animal Pasteurellaceae by MALDI-TOF mass spectrometry.
    J Microbiol Methods. 2012 Apr;89(1):1-7 PMID: 22343217
  149. Pulsed field gel electrophoresis typing of human and retail foodstuff Campylobacters: an Irish perspective.
    Food Microbiol. 2011 May;28(3):426-33 PMID: 21356447
  150. Comparing inactivation protocols of Yersinia organisms for identification with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
    Rapid Commun Mass Spectrom. 2012 Mar 30;26(6):710-4 PMID: 22328226
  151. Filamentous fungal characterizations by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
    J Appl Microbiol. 2010 Feb;108(2):375-85 PMID: 19659699
  152. Application of matrix-assisted laser desorption ionization-time of flight mass spectrometry for discrimination of Escherichia strains possessing highly conserved ribosomal RNA gene sequences.
    Biol Pharm Bull. 2011;34(3):430-2 PMID: 21372397
  153. Multiplex detection of microbial and plant toxins by immunoaffinity enrichment and matrix-assisted laser desorption/ionization mass spectrometry.
    Anal Chem. 2010 Apr 1;82(7):2916-24 PMID: 20199054
  154. Rapid identification of bacteria from bioMérieux BacT/ALERT blood culture bottles by MALDI-TOF MS.
    Br J Biomed Sci. 2013;70(4):149-55 PMID: 24400426
  155. Evaluation of matrix-assisted laser desorption ionization-time of flight mass spectrometry for identification of nocardia species.
    J Clin Microbiol. 2010 Nov;48(11):4015-21 PMID: 20861335
  156. Identification of Gallibacterium species by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evaluated by multilocus sequence analysis.
    Int J Med Microbiol. 2011 Aug;301(6):513-22 PMID: 21596619
  157. Dermatophyte identification using matrix-assisted laser desorption ionization-time of flight mass spectrometry.
    J Clin Microbiol. 2011 Dec;49(12):4067-71 PMID: 21956979
  158. Proteomic profiling of intact mycobacteria by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
    Anal Chem. 2004 Oct 1;76(19):5769-76 PMID: 15456297
  159. Proteomics in the study of the molecular taxonomy and epidemiology of bacterial pathogens.
    Electrophoresis. 2009 Jun;30 Suppl 1:S133-41 PMID: 19517493
  160. Rapid detection of Bacillus spore aerosol particles by direct in situ analysis using MALDI-TOF mass spectrometry.
    Lett Appl Microbiol. 2014 Aug;59(2):177-83 PMID: 24702137
  161. Successful identification of clinical dermatophyte and Neoscytalidium species by matrix-assisted laser desorption ionization-time of flight mass spectrometry.
    J Clin Microbiol. 2012 Jul;50(7):2277-81 PMID: 22535981
  162. Identification of Bacillus anthracis by using matrix-assisted laser desorption ionization-time of flight mass spectrometry and artificial neural networks.
    Appl Environ Microbiol. 2009 Nov;75(22):7229-42 PMID: 19767470
  163. Characterization of Aspergillus spores by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
    Rapid Commun Mass Spectrom. 2000;14(24):2393-400 PMID: 11114056
  164. Mass spectrometry based methods for the discrimination and typing of mycobacteria.
    Infect Genet Evol. 2012 Jun;12(4):838-45 PMID: 22230718
  165. Discrimination of Penicillium isolates by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry fingerprinting.
    Rapid Commun Mass Spectrom. 2008 Aug;22(16):2555-60 PMID: 18646251
  166. Evaluation of MALDI-TOF mass spectrometry and Sepsityper Kit™ for the direct identification of organisms from sterile body fluids in a Canadian pediatric hospital.
    Can J Infect Dis Med Microbiol. 2013 Winter;24(4):191-4 PMID: 24489560
  167. What is a species in Aspergillus?
    Med Mycol. 2009;47 Suppl 1:S13-20 PMID: 19255907
  168. Comparison of two matrix-assisted laser desorption ionization-time of flight mass spectrometry methods with conventional phenotypic identification for routine identification of bacteria to the species level.
    J Clin Microbiol. 2010 Apr;48(4):1169-75 PMID: 20164271
  169. mMass data miner: an open source alternative for mass spectrometric data analysis.
    Rapid Commun Mass Spectrom. 2008;22(6):905-8 PMID: 18293430
  170. An extraction method of positive blood cultures for direct identification of Candida species by Vitek MS matrix-assisted laser desorption ionization time of flight mass spectrometry.
    Med Mycol. 2013 Aug;51(6):652-6 PMID: 23373445
  171. MALDIquant: a versatile R package for the analysis of mass spectrometry data.
    Bioinformatics. 2012 Sep 1;28(17):2270-1 PMID: 22796955
  172. Direct identification of bacteria in positive blood culture bottles by matrix-assisted laser desorption ionisation time-of-flight mass spectrometry.
    PLoS One. 2009 Nov 25;4(11):e8041 PMID: 19946369
  173. Identification of Trueperella (Arcanobacterium) bernardiae by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis and by species-specific PCR.
    J Med Microbiol. 2012 Mar;61(Pt 3):457-9 PMID: 22096130
  174. Characterisation of single nucleotide polymorphisms in the genome of JC polyomavirus using MALDI TOF mass spectrometry.
    J Virol Methods. 2010 Mar;164(1-2):63-7 PMID: 19961879
  175. Progress with gene-product mapping of the Mollicutes: Mycoplasma genitalium.
    Electrophoresis. 1995 Jul;16(7):1090-4 PMID: 7498152
  176. Typing of human and animal strains of influenza virus with conserved signature peptides of matrix M1 protein by high resolution mass spectrometry.
    J Virol Methods. 2010 May;165(2):178-85 PMID: 20117137
  177. Proteotyping for the rapid identification of influenza virus and other biopathogens.
    Chem Soc Rev. 2013 Nov 21;42(22):8584-95 PMID: 23632861
  178. Gram-stain plus MALDI-TOF MS (Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry) for a rapid diagnosis of urinary tract infection.
    PLoS One. 2014 Jan 22;9(1):e86915 PMID: 24466289
  179. Identification of clinically relevant Corynebacterium spp., Arcanobacterium haemolyticum, and Rhodococcus equi by matrix-assisted laser desorption ionization-time of flight mass spectrometry.
    J Clin Microbiol. 2012 May;50(5):1745-7 PMID: 22337985
  180. Matrix-assisted laser desorption ionization (MALDI)-time of flight mass spectrometry- and MALDI biotyper-based identification of cultured biphenyl-metabolizing bacteria from contaminated horseradish rhizosphere soil.
    Appl Environ Microbiol. 2011 Oct;77(19):6858-66 PMID: 21821747
  181. Proteomics for routine identification of microorganisms.
    Proteomics. 2011 Aug;11(15):3143-53 PMID: 21726051
  182. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry and database for identification of Legionella species.
    Can J Microbiol. 2011 Jul;57(7):533-8 PMID: 21767077
  183. Effect of culture conditions on microorganism identification by matrix-assisted laser desorption ionization mass spectrometry.
    Appl Environ Microbiol. 2005 Jan;71(1):58-64 PMID: 15640170
  184. Differentiation of Campylobacter species by surface-enhanced laser desorption/ionization-time-of-flight mass spectrometry.
    Foodborne Pathog Dis. 2011 Aug;8(8):875-85 PMID: 21524195
  185. Rapid identification of Cardiobacterium hominis by MALDI-TOF mass spectrometry during infective endocarditis.
    Jpn J Infect Dis. 2011;64(4):327-9 PMID: 21788710
  186. Comparison of Bruker Biotyper matrix-assisted laser desorption ionization-time of flight mass spectrometer to BD Phoenix automated microbiology system for identification of gram-negative bacilli.
    J Clin Microbiol. 2011 Mar;49(3):887-92 PMID: 21209160
  187. Multiplex detection of human herpesviruses from archival specimens by using matrix-assisted laser desorption ionization-time of flight mass spectrometry.
    J Clin Microbiol. 2008 Feb;46(2):540-5 PMID: 18094141
  188. Application and use of various mass spectrometry methods in clinical microbiology.
    Clin Microbiol Infect. 2010 Nov;16(11):1604-13 PMID: 20969670
  189. The contribution of proteomics towards deciphering the enigma of Coxiella burnetii.
    Proteomics Clin Appl. 2013 Jan;7(1-2):193-204 PMID: 23255407
  190. Matrix-assisted laser desorption/ionisation-time of flight mass spectrometry: rapid identification of bacteria isolated from patients with cystic fibrosis.
    Br J Biomed Sci. 2013;70(4):144-8 PMID: 24400425
  191. Towards a natural system of organisms: proposal for the domains Archaea, Bacteria, and Eucarya.
    Proc Natl Acad Sci U S A. 1990 Jun;87(12):4576-9 PMID: 2112744
  192. Mass spectrometry and the age of the proteome.
    J Mass Spectrom. 1998 Jan;33(1):1-19 PMID: 9449829
  193. On species descriptions based on a single strain: proposal to introduce the status species proponenda (sp. pr.).
    Int J Syst Evol Microbiol. 2007 Sep;57(Pt 9):2185-7 PMID: 17766896
Article Info
Journal
Frontiers in microbiology
Abbr.
Front Microbiol
ISSN
1664-302X
DOI
10.3389/fmicb.2015.00791
Published
2015-00-00
Epub
2015-00-05
Pages
791
Language
English
Region
Switzerland
NLM ID
101548977
PMCID
PMC4525378
External Links
PubMed source DOI: 10.3389/fmicb.2015.00791 PMC Full Text View journal details
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