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PMID: 21655291 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

MALDI-TOF mass spectrometry is a fast and reliable platform for identification and ecological studies of species from family Rhizobiaceae.

PloS one ·Vol. 6 ·No. 5 ·2011-00-00 ·Pages e20223

Ferreira L, Sánchez-Juanes F, García-Fraile P, Rivas R, Mateos PF, Martínez-Molina E, González-Buitrago JM, Velázquez E

Abstract

Family Rhizobiaceae includes fast growing bacteria currently arranged into three genera, Rhizobium, Ensifer and Shinella, that contain pathogenic, symbiotic and saprophytic species. The identification of these species is not possible on the basis of physiological or biochemical traits and should be based on sequencing of several genes. Therefore alternative methods are necessary for rapid and reliable identification of members from family Rhizobiaceae. In this work we evaluated the suitability of Matrix-Assisted Laser Desorption Ionization-Time-of-Flight Mass Spectrometry (MALDI-TOF MS) for this purpose. Firstly, we evaluated the capability of this methodology to differentiate among species of family Rhizobiaceae including those closely related and then we extended the database of MALDI Biotyper 2.0 including the type strains of 56 species from genera Rhizobium, Ensifer and Shinella. Secondly, we evaluated the identification potential of this methodology by using several strains isolated from different sources previously identified on the basis of their rrs, recA and atpD gene sequences. The 100% of these strains were correctly identified showing that MALDI-TOF MS is an excellent tool for identification of fast growing rhizobia applicable to large populations of isolates in ecological and taxonomic studies.

MeSH Terms
Bacterial Proteins/genetics,metabolism Phylogeny Rhizobiaceae/classification,metabolism Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods
Chemicals
Bacterial Proteins
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Ferreira Laura
Unidad de Investigación, Hospital Universitario de Salamanca, Salamanca, Spain.
Sánchez-Juanes Fernando
García-Fraile Paula
Rivas Raúl
Mateos Pedro F
Martínez-Molina Eustoquio
González-Buitrago José Manuel
Velázquez Encarna
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Article Info
Journal
PloS one
Abbr.
PLoS One
ISSN
1932-6203
DOI
10.1371/journal.pone.0020223
Published
2011-00-00
Epub
2011-00-31
Pages
e20223
Language
English
Region
United States
NLM ID
101285081
PMCID
PMC3105015
Subset
IM
External Links
PubMed source DOI: 10.1371/journal.pone.0020223 PMC Full Text View journal details
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