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PMID: 2641278 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Nucleotide sequence and cellular distribution of rat chromogranin B (secretogranin I) mRNA in the neuroendocrine system.

Journal of molecular neuroscience : MN ·Vol. 1 ·No. 2 ·1989-00-00 ·Pages 63-75

Forss-Petter S, Danielson P, Battenberg E, Bloom F, Sutcliffe JG

Abstract

The mRNA of rat secretory-vesicle protein chromogranin B is abundant in brain, adrenal medulla, and anterior pituitary. The primary translation product predicted from the cDNA sequence of this 2,337-nucleotide transcript corresponds to a hydrophilic 655-residue protein preceded by a signal peptide. Both termini of the mature 75-kD protein show extensive similarity to other chromogranins; the more variable internal region is characterized by glutamic acid clusters and numerous pairs of basic residues. In rodent brain, mRNA accumulation starts around embryonic days 13-14 and peaks by postnatal day 20. In situ hybridization in brain sections shows that the mRNA is enriched in the hippocampal formation, the endocrine hypothalamus, the olfactory system, and in anatomically distinct structures in the pons-medulla.

MeSH Terms
Amino Acid Sequence Animals Animals, Newborn Base Sequence Chromogranin B Chromogranins/genetics Cloning, Molecular Embryo, Mammalian Gene Library Humans Mice Mice, Inbred BALB C Molecular Sequence Data Nerve Tissue Proteins/genetics Neurosecretory Systems/metabolism Nucleic Acid Hybridization Organ Specificity RNA, Messenger/analysis,genetics Rats Rats, Inbred Strains Restriction Mapping Sequence Homology, Nucleic Acid Transcription, Genetic
Chemicals
CHGB protein, human Chromogranin B Chromogranins Nerve Tissue Proteins RNA, Messenger chromogranin B, mouse chromogranin B, rat
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Forss-Petter S
Department of Molecular Biology, Scripps Clinic and Research Foundation, La Jolla, CA 92037.
Danielson P
Battenberg E
Bloom F
Sutcliffe J G
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Article Info
Journal
Journal of molecular neuroscience : MN
Abbr.
J Mol Neurosci
ISSN
0895-8696
DOI
10.1007/BF02896890
Published
1989-00-00
Pages
63-75
Language
English
Region
United States
NLM ID
9002991
Subset
IM
Grants
NINDS NIH HHS · NS 22111 · United States
NINDS NIH HHS · NS 22347 · United States
External Links
PubMed source DOI: 10.1007/BF02896890 View journal details
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