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PMID: 2645297 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Cell cycle-dependent association of HSP70 with specific cellular proteins.

The Journal of cell biology ·Vol. 108 ·No. 2 ·1989-02-00 ·Pages 413-23

Milarski KL, Welch WJ, Morimoto RI

Abstract

In asynchronous populations of HeLa cells maintained at control or heat shock temperatures, HSP70 levels and its subcellular distribution exhibit substantial heterogeneity as demonstrated by indirect immunofluorescence with HSP70-specific monoclonal antibodies. Of particular interest is a subpopulation of cells in which the characteristic nuclear accumulation and nucleolar association of HSP70 is not detected after heat shock treatment. This apparent variation in the heat shock response is not observed when synchronized cells are examined. In this study, we demonstrate that three monoclonal antibodies to HSP70, in particular, do not detect nucleolar-localized HSP70 in heat-shocked G2 cells. This is not due to an inability of G2 cells to respond to heat shock as measured by increased HSP70 mRNA and protein synthesis, or due to a lack of accumulation of HSP70 after heat shock in G2. Rather the epitopes recognized by the various antibodies appear to be inaccessible, perhaps due to the association of HSP70 with other proteins. Non-denaturing immunoprecipitations with these HSP70-specific antibodies suggest that HSP70 may interact with other cellular proteins in a cell cycle-dependent manner.

MeSH Terms
Antibodies, Monoclonal Cell Cycle Cell Nucleolus/metabolism Cell Nucleus/metabolism Electrophoresis, Polyacrylamide Gel Fluorescent Antibody Technique HeLa Cells Heat-Shock Proteins/metabolism Hot Temperature Humans Immunosorbent Techniques Interphase Proteins/metabolism RNA, Messenger/biosynthesis
Chemicals
Antibodies, Monoclonal Heat-Shock Proteins Proteins RNA, Messenger
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Milarski K L
Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, Illinois 60208.
Welch W J
Morimoto R I
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.108.2.413
Published
1989-02-00
Pages
413-23
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2115428
Subset
IM
Grants
NIGMS NIH HHS · GM 08061 · United States
External Links
PubMed source DOI: 10.1083/jcb.108.2.413 PMC Full Text View journal details
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