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PMID: 3275876 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Complex regulation of heat shock- and glucose-responsive genes in human cells.

Molecular and cellular biology ·Vol. 8 ·No. 1 ·1988-01-00 ·Pages 393-405

Watowich SS, Morimoto RI

Abstract

We have isolated a human genomic clone that encodes the glucose-responsive protein GRP78 and have used this cloned gene probe, together with a cloned HSP70 gene, to study the expression of both stress-induced genes in response to inhibitors of cellular metabolism. On the basis of the effects of this group of chemicals on GRP78 and HSP70 expression, we have identified three classes of stress gene inducers. The first class induces GRP78 expression and includes inhibitors of glycoprotein processing. The second class results in coordinate activation of both GRP78 and HSP70 synthesis and includes amino acid analogs and heavy metals. Chemicals in the third class coordinately induce GRP78 and repress HSP70 expression; this class includes the calcium ionophore A23187 and the glucose analog 2-deoxyglucose. Whereas induction of GRP78 or HSP70 expression is primarily due to transcriptional activation, chemicals that repress HSP70 expression act through posttranscriptional regulation. These results reveal that the regulation of GRP78 and HSP70 expression is complex and may be dependent on the specificity and magnitude of physiological damage.

MeSH Terms
Azetidines/pharmacology Cloning, Molecular Deoxy Sugars/pharmacology Deoxyglucose/pharmacology Endoplasmic Reticulum Chaperone BiP Gene Expression Regulation/drug effects Genes HSP70 Heat-Shock Proteins Heat-Shock Proteins/genetics,immunology Hot Temperature Humans Immunologic Techniques Membrane Proteins/genetics,immunology Multigene Family Nucleic Acid Hybridization RNA, Messenger/genetics Sequence Homology, Nucleic Acid Tunicamycin/pharmacology
Chemicals
Azetidines Deoxy Sugars Endoplasmic Reticulum Chaperone BiP HSP70 Heat-Shock Proteins HSPA5 protein, human Heat-Shock Proteins Membrane Proteins RNA, Messenger glucose-regulated proteins Tunicamycin Deoxyglucose
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Watowich S S
Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, Illinois 60208.
Morimoto R I
References (53)
53 references, click to expand
  1. Impaired intracellular migration and altered solubility of nonglycosylated glycoproteins of vesicular stomatitis virus and Sindbis virus.
    J Biol Chem. 1977 Dec 25;252(24):9018-23 PMID: 200626
  2. Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange.
    Proc Natl Acad Sci U S A. 1977 Nov;74(11):4835-8 PMID: 73185
  3. Role of carbohydrates in protein secretion and turnover: effects of tunicamycin on the major cell surface glycoprotein of chick embryo fibroblasts.
    Cell. 1978 Mar;13(3):461-73 PMID: 657267
  4. Transcription and translation of Newcastle disease virus mRNA's in vitro.
    J Virol. 1978 Oct;28(1):324-36 PMID: 702653
  5. The isolation of structural genes from libraries of eucaryotic DNA.
    Cell. 1978 Oct;15(2):687-701 PMID: 719759
  6. Sequence organization and transcription at two heat shock loci in Drosophila.
    Proc Natl Acad Sci U S A. 1978 Nov;75(11):5613-7 PMID: 103099
  7. The isolation and characterization of linked delta- and beta-globin genes from a cloned library of human DNA.
    Cell. 1978 Dec;15(4):1157-74 PMID: 728996
  8. Infection with paramyxoviruses stimulates synthesis of cellular polypeptides that are also stimulated in cells transformed by Rous sarcoma virus or deprived of glucose.
    Proc Natl Acad Sci U S A. 1978 Dec;75(12):6120-4 PMID: 216013
  9. Sequence organization of two recombinant plasmids containing genes for the major heat shock-induced protein of D. melanogaster.
    Cell. 1979 Mar;16(3):575-88 PMID: 110452
  10. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
    Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201-5 PMID: 6159641
  11. Selective stimulation of the synthesis of an 80,000-dalton protein by calcium ionophores.
    J Biol Chem. 1981 Jun 10;256(11):5309-12 PMID: 6165714
  12. "Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate--polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A.
    Anal Biochem. 1981 Apr;112(2):195-203 PMID: 6266278
  13. Highly conserved glucose-regulated protein in hamster and chicken cells: preliminary characterization of its cDNA clone.
    Proc Natl Acad Sci U S A. 1981 Aug;78(8):4922-5 PMID: 6946438
  14. Drosophila gene related to the major heat shock-induced gene is transcribed at normal temperatures and not induced by heat shock.
    Proc Natl Acad Sci U S A. 1982 Jan;79(2):525-9 PMID: 6804941
  15. A major cytoplasmic glucose-regulated protein is associated with the Rous sarcoma virus pp60src protein.
    J Biol Chem. 1982 Aug 10;257(15):8604-7 PMID: 6807976
  16. Saccharomyces cerevisiae contains a complex multigene family related to the major heat shock-inducible gene of Drosophila.
    Mol Cell Biol. 1982 Nov;2(11):1388-98 PMID: 6761581
  17. Biochemical characterization of the mammalian stress proteins and identification of two stress proteins as glucose- and Ca2+-ionophore-regulated proteins.
    J Biol Chem. 1983 Jun 10;258(11):7102-11 PMID: 6406494
  18. Isolation and characterization of full-length cDNA clones for human alpha-, beta-, and gamma-actin mRNAs: skeletal but not cytoplasmic actins have an amino-terminal cysteine that is subsequently removed.
    Mol Cell Biol. 1983 May;3(5):787-95 PMID: 6865942
  19. The effects of glucose on protein synthesis and thermosensitivity in Chinese hamster ovary cells.
    J Biol Chem. 1983 Oct 25;258(20):12091-3 PMID: 6630181
  20. Immunoglobulin heavy chain binding protein.
    Nature. 1983 Nov 24-30;306(5941):387-9 PMID: 6417546
  21. Transcription and post-transcriptional regulation of avian HSP70 gene expression.
    J Biol Chem. 1986 Nov 25;261(33):15740-5 PMID: 3782087
  22. Calcium ionophore A23187 as a regulator of gene expression in mammalian cells.
    J Cell Biol. 1986 Dec;103(6 Pt 1):2145-52 PMID: 3097027
  23. Expression of human HSP70 during the synthetic phase of the cell cycle.
    Proc Natl Acad Sci U S A. 1986 Dec;83(24):9517-21 PMID: 3540942
  24. Chromosomal location of human genes encoding major heat-shock protein HSP70.
    Somat Cell Mol Genet. 1987 Mar;13(2):119-30 PMID: 3470951
  25. Posttranscriptional regulation of hsp70 expression in human cells: effects of heat shock, inhibition of protein synthesis, and adenovirus infection on translation and mRNA stability.
    Mol Cell Biol. 1987 Dec;7(12):4357-68 PMID: 3437893
  26. Transcriptional activation and subsequent control of the human heat shock gene during adenovirus infection.
    Mol Cell Biol. 1983 Nov;3(11):2058-65 PMID: 6689197
  27. Major heat shock gene of Drosophila and the Escherichia coli heat-inducible dnaK gene are homologous.
    Proc Natl Acad Sci U S A. 1984 Feb;81(3):848-52 PMID: 6322174
  28. SV40 T antigen and the exocytotic pathway.
    EMBO J. 1985 Jun;4(6):1479-89 PMID: 3849428
  29. Rapid purification of mammalian 70,000-dalton stress proteins: affinity of the proteins for nucleotides.
    Mol Cell Biol. 1985 Jun;5(6):1229-37 PMID: 4033650
  30. Transcription of the human hsp70 gene is induced by serum stimulation.
    Proc Natl Acad Sci U S A. 1985 Sep;82(18):6070-4 PMID: 3862119
  31. The heat shock response.
    CRC Crit Rev Biochem. 1985;18(3):239-80 PMID: 2412760
  32. Conserved features of eukaryotic hsp70 genes revealed by comparison with the nucleotide sequence of human hsp70.
    Proc Natl Acad Sci U S A. 1985 Oct;82(19):6455-9 PMID: 3931075
  33. The 70-kd mammalian heat shock proteins are structurally and functionally related to the uncoating protein that releases clathrin triskelia from coated vesicles.
    EMBO J. 1985 Dec 16;4(13A):3385-91 PMID: 2868889
  34. Uncoating ATPase is a member of the 70 kilodalton family of stress proteins.
    Cell. 1986 Apr 11;45(1):3-13 PMID: 2937542
  35. Posttranslational association of immunoglobulin heavy chain binding protein with nascent heavy chains in nonsecreting and secreting hybridomas.
    J Cell Biol. 1986 May;102(5):1558-66 PMID: 3084497
  36. An Hsp70-like protein in the ER: identity with the 78 kd glucose-regulated protein and immunoglobulin heavy chain binding protein.
    Cell. 1986 Jul 18;46(2):291-300 PMID: 3087629
  37. The heat-shock response.
    Annu Rev Biochem. 1986;55:1151-91 PMID: 2427013
  38. Expression of wild-type and mutant forms of influenza hemagglutinin: the role of folding in intracellular transport.
    Cell. 1986 Sep 12;46(6):939-50 PMID: 3757030
  39. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  40. High resolution two-dimensional electrophoresis of proteins.
    J Biol Chem. 1975 May 25;250(10):4007-21 PMID: 236308
  41. Inhibition of the multiplication of enveloped viruses by glucose derivatives.
    Curr Top Microbiol Immunol. 1975;70:101-19 PMID: 808397
  42. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  43. An efficient mRNA-dependent translation system from reticulocyte lysates.
    Eur J Biochem. 1976 AUG 1;67(1):247-56 PMID: 823012
  44. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
    J Mol Biol. 1977 Jun 15;113(1):237-51 PMID: 881736
  45. Induction of two transformation-sensitive membrane polypeptides in normal fibroblasts by a block in glycoprotein synthesis or glucose deprivation.
    Cell. 1977 Aug;11(4):941-7 PMID: 196769
  46. Highly specific transcription of globin sequences in isolated reticulocyte nuclei.
    Biochem Biophys Res Commun. 1977 Aug 22;77(4):1478-85 PMID: 901547
  47. Glucose depletion accounts for the induction of two transformation-sensitive membrane proteinsin Rous sarcoma virus-transformed chick embryo fibroblasts.
    Proc Natl Acad Sci U S A. 1977 Sep;74(9):3840-4 PMID: 198809
  48. Nuclear and nucleolar localization of the 72,000-dalton heat shock protein in heat-shocked mammalian cells.
    J Biol Chem. 1984 Apr 10;259(7):4501-13 PMID: 6368558
  49. Proteins related to the mouse L-cell major heat shock protein are synthesized in the absence of heat shock gene expression.
    Proc Natl Acad Sci U S A. 1984 Apr;81(8):2317-21 PMID: 6201852
  50. Induction of glucose-regulated proteins during anaerobic exposure and of heat-shock proteins after reoxygenation.
    Proc Natl Acad Sci U S A. 1984 Aug;81(15):4843-7 PMID: 6589630
  51. Heat shock-induced translational control of HSP70 and globin synthesis in chicken reticulocytes.
    Mol Cell Biol. 1984 Nov;4(11):2437-48 PMID: 6513924
  52. Structure and expression of the human gene encoding major heat shock protein HSP70.
    Mol Cell Biol. 1985 Feb;5(2):330-41 PMID: 2858050
  53. The effect of castanospermine on the oligosaccharide structures of glycoproteins from lymphoma cell lines.
    Biochem J. 1985 May 1;227(3):795-804 PMID: 3924028
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.8.1.393-405.1988
Published
1988-01-00
Pages
393-405
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC363136
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.8.1.393-405.1988 PMC Full Text View journal details
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