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PMID: 2653216 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Chromosomal beta-lactamase of Klebsiella oxytoca, a new class A enzyme that hydrolyzes broad-spectrum beta-lactam antibiotics.

Antimicrobial agents and chemotherapy ·Vol. 33 ·No. 1 ·1989-01-00 ·Pages 63-70

Arakawa Y, Ohta M, Kido N, Mori M, Ito H, Komatsu T, Fujii Y, Kato N

Abstract

The chromosomally encoded beta-lactamase gene of Klebsiella oxytoca E23004, a strain resistant to cefoperazone and aztreonam, was cloned and expressed in Escherichia coli HB101. The molecular mass and pI of this enzyme were 28 kilodaltons and 7.4, respectively. Although the beta-lactamase of K. oxytoca hydrolyzed many cephalosporins, including broad-spectrum drugs, the nucleotide sequence and deduced amino acid sequence lacked homology with chromosomal class C beta-lactamase genes (ampC) of E. coli or Citrobacter freundii. Rather, about 45% nucleotide sequence homology and 40% deduced amino acid sequence homology were observed between the K. oxytoca beta-lactamase and TEM-1, a class A beta-lactamase which does not efficiently hydrolyze cephalosporins. Values of Km, relative Vmax, and relative Vmax/Km for the K. oxytoca beta-lactamase indicated that the enzyme is a penicillinase but that it can hydrolyze cefoperazone effectively and other broad-spectrum cephems weakly. Hence, the chromosomal beta-lactamase of K. oxytoca E23004 belongs to class A but differences in its amino acid sequence provide a broader spectrum of activity.

MeSH Terms
Ampicillin/pharmacology Base Sequence Cefotaxime/pharmacology Cephaloridine/pharmacology Chromosomes, Bacterial/metabolism Cloning, Molecular DNA, Bacterial/analysis Drug Resistance, Microbial/genetics Escherichia coli/enzymology,genetics Klebsiella/enzymology,genetics Klebsiella pneumoniae/enzymology,genetics Restriction Mapping beta-Lactamases/analysis,genetics,metabolism
Chemicals
DNA, Bacterial Ampicillin beta-Lactamases Cephaloridine Cefotaxime
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Arakawa Y
Department of Bacteriology, Nagoya University School of Medicine, Japan.
Ohta M
Kido N
Mori M
Ito H
Komatsu T
Fujii Y
Kato N
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Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.33.1.63
Published
1989-01-00
Pages
63-70
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC171422
Subset
IM
Databases
GENBANK
M27459
Corrections
ErratumIn
-
External Links
PubMed source DOI: 10.1128/AAC.33.1.63 PMC Full Text View journal details
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