gyrA and gyrB mutations in quinolone-resistant strains of Escherichia coli.

Antimicrobial agents and chemotherapy ·Vol. 33 ·No. 2 ·1989-02-00 ·Pages 254-5

Nakamura S, Nakamura M, Kojima T, Yoshida H

Abstract

The proportion of gyrA and gyrB mutations in quinolone-resistant Escherichia coli strains was examined by introducing cloned wild-type gyrA and gyrB genes. In 25 spontaneous mutants of strain KL16, 13 had gyrA and 12 had gyrB mutations. In eight clinical isolates, five had gyrA mutations and one had a gyrB mutation; mutations in two isolates remained unidentified.

MeSH Terms

Drug Resistance, Microbial Escherichia coli/drug effects,genetics Genes, Bacterial Mutation Plasmids Quinolines/pharmacology

Chemicals

Quinolines

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Nakamura S

Research Laboratories, Dainippon Pharmaceutical Co., Ltd., Osaka, Japan.

Nakamura M

Kojima T

Yoshida H

References (12)

12 references, click to expand

Nalidixic acid-resistant mutations of the gyrB gene of Escherichia coli.
Mol Gen Genet. 1986 Sep;204(3):367-73 PMID: 3020376
Isolation and characterization of norfloxacin-resistant mutants of Escherichia coli K-12.
Antimicrob Agents Chemother. 1986 Aug;30(2):248-53 PMID: 3532944
Antagonism of wild-type and resistant Escherichia coli and its DNA gyrase by the tricyclic 4-quinolone analogs ofloxacin and S-25930 stereoisomers.
Antimicrob Agents Chemother. 1987 Nov;31(11):1861-3 PMID: 2829717
Quinolone-resistant mutations of the gyrA gene of Escherichia coli.
Mol Gen Genet. 1988 Jan;211(1):1-7 PMID: 2830458
Escherichia coli K-12 mutants resistant to nalidixic acid: genetic mapping and dominance studies.
J Bacteriol. 1969 Jul;99(1):238-41 PMID: 4895844
Genetic and biochemical characterization of norfloxacin resistance in Escherichia coli.
Antimicrob Agents Chemother. 1986 Apr;29(4):639-44 PMID: 3010850
Purification of subunits of Escherichia coli DNA gyrase and reconstitution of enzymatic activity.
Proc Natl Acad Sci U S A. 1978 Apr;75(4):1773-7 PMID: 347446
Hybrid plasmids containing an active thymidine kinase gene of Herpes simplex virus 1.
Nucleic Acids Res. 1979 Oct 25;7(4):859-77 PMID: 228250
New nalidixic acid resistance mutations related to deoxyribonucleic acid gyrase activity.
J Bacteriol. 1981 Nov;148(2):450-8 PMID: 6271730
Selection of multiple antibiotic resistance by quinolones, beta-lactams, and aminoglycosides with special reference to cross-resistance between unrelated drug classes.
Antimicrob Agents Chemother. 1984 Dec;26(6):797-801 PMID: 6098219
Mutation affecting resistance of Escherichia coli K12 to nalidixic acid.
J Gen Microbiol. 1985 Sep;131(9):2285-92 PMID: 3906038
Studies on the mechanism of action of nalidixic acid.
Antimicrob Agents Chemother. 1973 Oct;4(4):479-86 PMID: 4208771

Article Info

Journal

Antimicrobial agents and chemotherapy

Abbr.

Antimicrob Agents Chemother

ISSN

0066-4804

DOI

10.1128/AAC.33.2.254

Published

1989-02-00

Pages

254-5

Language

English

Region

United States

NLM ID

0315061

PMCID

PMC171470

Subset

External Links

PubMed source DOI: 10.1128/AAC.33.2.254 PMC Full Text View journal details

Analysis Services

gyrA and gyrB mutations in quinolone-resistant strains of Escherichia coli.

Contact

WeChat Official Account