Home LiteratureArticle Details
PMID: 2671664 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Characterization of the ColE1 mobilization region and its protein products.

Molecular & general genetics : MGG ·Vol. 217 ·No. 2-3 ·1989-06-00 ·Pages 488-98

Boyd AC, Archer JA, Sherratt DJ

Abstract

A third of the 6.6 kb genome of ColE1 is devoted to mobilization (mob) genes necessary to promote its specific transfer in the presence of conjugative plasmids. The mob region is genetically complex: two mob genes are entirely overlapped by a third. Oligonucleotide-directed mutagenesis was used to insert an amber codon into one of the overlapped genes and make possible a full complementation analysis of mob. Four mob genes essential for mobilization by R64drd11 were thus identified. Fragments of mob were subcloned under control of the Ptac promoter in a suitable vector, overexpressed in minicells and the mobilization proteins visualized. A comprehensive alignment of the mob region of ColE1 with those of its close relatives ColK and ColA demonstrating that the four essential mob genes are conserved is also presented.

MeSH Terms
Amino Acid Sequence Base Sequence Colicins/genetics Conjugation, Genetic DNA, Bacterial/genetics Escherichia coli/genetics Gene Expression Regulation Genes, Bacterial Molecular Sequence Data Plasmids Sequence Homology, Nucleic Acid
Chemicals
Colicins DNA, Bacterial
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Boyd A C
Department of Molecular Biology, University of Edinburgh, UK.
Archer J A
Sherratt D J
References (42)
42 references, click to expand
  1. Proteins synthesized in minicells containing plasmid ColE1 and its mutants.
    J Bacteriol. 1978 Mar;133(3):1444-51 PMID: 346572
  2. The complete nucleotide sequence of the colicinogenic plasmid ColA. High extent of homology with ColE1.
    Mol Gen Genet. 1988 Feb;211(2):231-43 PMID: 2832701
  3. Gene expression in vitro of colicin El plasmid.
    Nucleic Acids Res. 1979 Oct 10;7(3):639-49 PMID: 388345
  4. Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.
    Proc Natl Acad Sci U S A. 1969 Apr;62(4):1159-66 PMID: 4894690
  5. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  6. Search algorithm for pattern match analysis of nucleic acid sequences.
    Nucleic Acids Res. 1983 May 11;11(9):2943-57 PMID: 6344023
  7. Vectors bearing a hybrid trp-lac promoter useful for regulated expression of cloned genes in Escherichia coli.
    Gene. 1983 Nov;25(2-3):167-78 PMID: 6363212
  8. Isolation and characterization of Co1E2 plasmid mutants unable to kill colicin-sensitive cells.
    Mol Gen Genet. 1976 Aug 2;146(3):239-45 PMID: 794689
  9. An optimized freeze-squeeze method for the recovery of DNA fragments from agarose gels.
    Anal Biochem. 1983 Jul 1;132(1):14-9 PMID: 6312834
  10. The transposon Tn1 as a probe for studying ColE1 structure and function.
    Mol Gen Genet. 1977 Mar 7;151(2):151-60 PMID: 327263
  11. A system for shotgun DNA sequencing.
    Nucleic Acids Res. 1981 Jan 24;9(2):309-21 PMID: 6259625
  12. Nucleotide sequence and gene organization of ColE1 DNA.
    J Biol Chem. 1985 Jul 25;260(15):8925-35 PMID: 2991225
  13. Multimer resolution systems of ColE1 and ColK: localisation of the crossover site.
    Mol Gen Genet. 1985;201(2):334-8 PMID: 3003536
  14. Processing of plasmid DNA during bacterial conjugation.
    Microbiol Rev. 1984 Mar;48(1):24-41 PMID: 6201705
  15. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.
    Gene. 1982 Oct;19(3):259-68 PMID: 6295879
  16. Cell-free synthesis of proteins coding for mobilisation functions of ColE1 and transposition functions of Tn3.
    Gene. 1979 May;6(1):29-42 PMID: 383577
  17. A promoter of pBR322 activated by cAMP receptor protein.
    Nucleic Acids Res. 1981 Jul 24;9(14):3365-77 PMID: 6269081
  18. Use of the 'Perceptron' algorithm to distinguish translational initiation sites in E. coli.
    Nucleic Acids Res. 1982 May 11;10(9):2997-3011 PMID: 7048259
  19. Nucleotide sequence of bacteriophage lambda DNA.
    J Mol Biol. 1982 Dec 25;162(4):729-73 PMID: 6221115
  20. The conjugation system of F, the fertility factor of Escherichia coli.
    Annu Rev Genet. 1986;20:593-624 PMID: 2880557
  21. Control of ColE1 DNA replication: the rop gene product negatively affects transcription from the replication primer promoter.
    Proc Natl Acad Sci U S A. 1982 Oct;79(20):6313-7 PMID: 6183660
  22. A comprehensive set of sequence analysis programs for the VAX.
    Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387-95 PMID: 6546423
  23. A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
    Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 PMID: 388356
  24. pHG165: a pBR322 copy number derivative of pUC8 for cloning and expression.
    Plasmid. 1986 May;15(3):172-81 PMID: 3012611
  25. Analysis of ColE1 expression in vitro after chromosome fragmentation.
    J Bacteriol. 1983 May;154(2):650-5 PMID: 6188745
  26. Toxicity of an overproduced foreign gene product in Escherichia coli and its use in plasmid vectors for the selection of transcription terminators.
    Gene. 1984 Feb;27(2):161-72 PMID: 6202587
  27. Structure and nucleotide sequence of the region encoding the mobilization proteins of plasmid CloDF13.
    Gene. 1987;51(2-3):171-8 PMID: 3596243
  28. The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites.
    Proc Natl Acad Sci U S A. 1974 Apr;71(4):1342-6 PMID: 4598299
  29. Plasmid ColE1 conjugal mobility: the nature of bom, a region required in cis for transfer.
    Mol Gen Genet. 1982;185(2):344-51 PMID: 7045589
  30. Oligonucleotide-directed mutagenesis by microscale 'shot-gun' gene synthesis.
    Nucleic Acids Res. 1985 May 10;13(9):3305-16 PMID: 3889852
  31. Pedigrees of some mutant strains of Escherichia coli K-12.
    Bacteriol Rev. 1972 Dec;36(4):525-57 PMID: 4568763
  32. Trans-complementable copy-number mutants of plasmid ColE1.
    Nature. 1980 Jan 10;283(5743):216-8 PMID: 7350544
  33. A proposal for a uniform nomenclature in bacterial genetics.
    Genetics. 1966 Jul;54(1):61-76 PMID: 5961488
  34. Induced mutagenesis in dam- mutants of Escherichia coli: a role for 6-methyladenine residues in mutation avoidance.
    Mol Gen Genet. 1978 Jul 25;163(3):307-12 PMID: 355857
  35. Polar mobilization of the Escherichia coli chromosome by the ColE1 transfer origin.
    Mol Gen Genet. 1986 Jun;203(3):496-504 PMID: 3018432
  36. Relaxation complexes of plasmid DNA and protein. II. Characterization of the proteins associated with the unrelaxed and relaxed complexes of plasmid ColE1.
    J Biol Chem. 1975 Nov 25;250(22):8790-5 PMID: 1102544
  37. Complementation of transfer deficient ColE1 mutants.
    Mol Gen Genet. 1977 Mar 7;151(2):197-201 PMID: 327266
  38. Natural variation in the genetic code.
    Annu Rev Genet. 1987;21:67-91 PMID: 3327473
  39. Isolation and computer-aided characterization of MmeI, a type II restriction endonuclease from Methylophilus methylotrophus.
    Nucleic Acids Res. 1986 Jul 11;14(13):5255-74 PMID: 3016643
  40. Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis.
    Gene. 1983 Dec;26(1):101-6 PMID: 6323249
  41. Translational coupling during expression of the tryptophan operon of Escherichia coli.
    Genetics. 1980 Aug;95(4):785-95 PMID: 6162715
  42. Identification of functional regions of the colicinogenic plasmid ColA.
    Mol Gen Genet. 1988 Feb;211(2):223-30 PMID: 2832700
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF02464922
Published
1989-06-00
Pages
488-98
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Databases
GENBANK
X15873
External Links
PubMed source DOI: 10.1007/BF02464922
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2