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PMID: 2674938 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Codon choice and gene expression: synonymous codons differ in translational accuracy.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 18 ·1989-09-00 ·Pages 6888-92

Dix DB, Thompson RC

Abstract

Ribosomes programmed by different synonymous codons also differ in discriminating among near-cognate aminoacylated tRNAs. In the initial step of the recognition reaction ribosomes programmed by UUC discriminate less well than ribosomes programmed by UUU against ternary complexes containing three types of Leu-tRNA, and ribosomes programmed by CUC discriminate less well than ribosomes programmed by CUU against ternary complexes containing Phe-tRNA. Furthermore, in the proofreading step ribosomes programmed by UUC discriminate less well than ribosomes programmed by UUU against two of three near-cognate Leu-tRNAs, and ribosomes programmed by CUC discriminate less well than ribosomes programmed by CUU against near-cognate Phe-tRNA. The codon-induced change in reaction rate with near-cognate ternary complexes is greater than that with cognate ternary complexes: the most efficient codon is, therefore, the least accurate. Because the efficient, but inaccurate, codon UUC is used preferentially in highly expressed mRNAs of Escherichia coli, maximization of translational accuracy apparently has not been significant in the evolution of this particular biased codon choice in E. coli.

MeSH Terms
Anticodon/genetics Base Sequence Codon/genetics Guanosine 5'-O-(3-Thiotriphosphate) Guanosine Triphosphate/analogs & derivatives,metabolism Kinetics Peptide Elongation Factor Tu/metabolism Protein Biosynthesis RNA, Messenger/genetics,metabolism RNA, Transfer/genetics,metabolism RNA, Transfer, Leu/metabolism RNA, Transfer, Phe/metabolism Ribosomes/metabolism Thionucleotides/metabolism
Chemicals
Anticodon Codon RNA, Messenger RNA, Transfer, Leu RNA, Transfer, Phe Thionucleotides Guanosine 5'-O-(3-Thiotriphosphate) Guanosine Triphosphate RNA, Transfer Peptide Elongation Factor Tu
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Dix D B
Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder 80309.
Thompson R C
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.86.18.6888
Published
1989-09-00
Pages
6888-92
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC297955
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.86.18.6888 PMC Full Text View journal details
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