Home LiteratureArticle Details
PMID: 2680757 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

A general screen for mutant of Saccharomyces cerevisiae deficient in tRNA biosynthesis.

Genetics ·Vol. 123 ·No. 1 ·1989-09-00 ·Pages 55-68

van Zyl WH, Wills N, Broach JR

Abstract

We have devised a general screen for isolating conditional lethal mutants defective in synthesis of mature tRNA in Saccharomyces cerevisiae. Using this screen, we have identified several new genes in yeast that are required for production of mature tRNA. These genes most likely encode essential functions, since the mutations we isolated are recessive and cause temperature-sensitive growth. One of the mutants, tpd3, is defective in de novo transcription of 4S RNA at the nonpermissive temperature. A second mutant, tpd1, is specifically defective in excision of intervening sequence from a variety of tRNA species. Finally, two other mutants are defective in production of tRNA from a suppressor tRNA locus, as measured by an in vitro suppression assay. The specific lesion in these strains, though, is not known. These data confirm that the screen does, in fact, yield a broad spectrum of mutants defective in tRNA maturation.

MeSH Terms
Blotting, Northern Gene Expression Regulation Genes, Lethal Mutation RNA, Transfer/biosynthesis Saccharomyces cerevisiae/genetics Suppression, Genetic
Chemicals
RNA, Transfer
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
van Zyl W H
Biology Department, Princeton University, New Jersey 08544.
Wills N
Broach J R
References (25)
25 references, click to expand
  1. Macromolecule synthesis in temperature-sensitive mutants of yeast.
    J Bacteriol. 1967 May;93(5):1662-70 PMID: 5337848
  2. Transfer RNA modification.
    Annu Rev Biochem. 1987;56:263-87 PMID: 3304135
  3. Mutants of Escherichia coli thermosensitive for the synthesis of transfer RNA.
    Proc Natl Acad Sci U S A. 1973 Jul;70(7):2091-5 PMID: 4579013
  4. Temperature-sensitive nonsense suppressors in yeast.
    Genetics. 1973 Nov;75(3):459-64 PMID: 4590686
  5. Beta-D-fructofuranoside fructohydrolase from yeast.
    Methods Enzymol. 1975;42:504-11 PMID: 237205
  6. Preparation of RNA and ribosomes from yeast.
    Methods Cell Biol. 1975;12:45-64 PMID: 1105073
  7. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
    Anal Biochem. 1976 May 7;72:248-54 PMID: 942051
  8. Identification of amber and ochre mutants of the human virus Ad2+ND1.
    Proc Natl Acad Sci U S A. 1977 Oct;74(10):4567-71 PMID: 200921
  9. Mutants of Saccharomyces cerevisiae unresponsive to cell division control by polypeptide mating hormone.
    J Cell Biol. 1980 Jun;85(3):811-22 PMID: 6993497
  10. Two differentially regulated mRNAs with different 5' ends encode secreted with intracellular forms of yeast invertase.
    Cell. 1982 Jan;28(1):145-54 PMID: 7039847
  11. Defects in modification of cytoplasmic and mitochondrial transfer RNAs are caused by single nuclear mutations.
    Cell. 1982 Mar;28(3):543-50 PMID: 7074684
  12. Precise excision of intervening sequences from precursor tRNAs by a membrane-associated yeast endonuclease.
    Cell. 1983 Feb;32(2):525-36 PMID: 6186398
  13. Construction and use of gene fusions to lacZ (beta-galactosidase) that are expressed in yeast.
    Methods Enzymol. 1983;101:167-80 PMID: 6310320
  14. One-step gene disruption in yeast.
    Methods Enzymol. 1983;101:202-11 PMID: 6310324
  15. Beta-galactosidase gene fusions for analyzing gene expression in escherichia coli and yeast.
    Methods Enzymol. 1983;100:293-308 PMID: 6312261
  16. Effects of altered 5'-flanking sequences on the in vivo expression of a Saccharomyces cerevisiae tRNATyr gene.
    Mol Cell Biol. 1984 Apr;4(4):657-65 PMID: 6371493
  17. Regulation of mating-type information in yeast. Negative control requiring sequences both 5' and 3' to the regulated region.
    J Mol Biol. 1984 Jul 5;176(3):307-31 PMID: 6379190
  18. Processing of tRNA in prokaryotes and eukaryotes.
    CRC Crit Rev Biochem. 1984;17(1):45-71 PMID: 6094100
  19. A positive selection for mutants lacking orotidine-5'-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance.
    Mol Gen Genet. 1984;197(2):345-6 PMID: 6394957
  20. Saccharomyces cerevisiae tRNA ligase. Purification of the protein and isolation of the structural gene.
    J Biol Chem. 1986 Feb 25;261(6):2978-86 PMID: 3512545
  21. A position effect on the expression of a tRNA gene mediated by the SIR genes in Saccharomyces cerevisiae.
    Mol Cell Biol. 1986 Feb;6(2):494-501 PMID: 3023851
  22. RPC40, a unique gene for a subunit shared between yeast RNA polymerases A and C.
    Cell. 1987 Feb 27;48(4):627-37 PMID: 3815519
  23. Molecular characterization of the yeast PRT1 gene in which mutations affect translation initiation and regulation of cell proliferation.
    J Biol Chem. 1987 Feb 25;262(6):2845-51 PMID: 3029094
  24. Cloning and characterization of LOS1, a Saccharomyces cerevisiae gene that affects tRNA splicing.
    Mol Cell Biol. 1987 Mar;7(3):1208-16 PMID: 3031485
  25. Studies on microbial ribonucleic acid. IV. Two mutants of Saccharomyces cerevisiae lacking N-2-dimethylguanine in soluble ribonucleic acid.
    J Mol Biol. 1967 Jun 28;26(3):509-18 PMID: 6029741
Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/123.1.55
Published
1989-09-00
Pages
55-68
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1203791
Subset
IM
Grants
NIGMS NIH HHS · GM34545 · United States
External Links
PubMed source DOI: 10.1093/genetics/123.1.55 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2