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PMID: 2681160 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Role of the CheW protein in bacterial chemotaxis: overexpression is equivalent to absence.

Journal of bacteriology ·Vol. 171 ·No. 11 ·1989-11-00 ·Pages 6271-8

Sanders DA, Mendez B, Koshland DE

Abstract

The cheW gene from Escherichia coli has been cloned an inducible promoter, and the effects of the overproduction of the CheW protein on chemotactic behavior and receptor covalent modification have been examined. Plasmids that contain the cheW gene behind a regulatable promoter complement a cheW mutation when the CheW protein is produced at low levels. However, when the CheW protein is greatly overproduced in either a wild-type strain or a cheW mutant, chemotaxis is greatly inhibited, cheW null mutant cells swim smoothly as if they were constantly responding to an attractant. Surprisingly, cells in which the CheW protein is overproduced also swim smoothly. The behavioral defect produced by overproduction of the CheW protein does not require the presence of the cheR, cheB, or cheZ gene. Receptor demethylation is also inhibited by overproduction of the CheW protein, as it is by a mutation in the cheW gene or a response to an attractant. In all respects, therefore, overproduction of the CheW protein has the same consequences as does a mutation in the cheW gene or a response to an attractant. A model involving two states of the CheW protein is proposed to explain its role in bacterial chemotaxis.

MeSH Terms
Bacterial Proteins/biosynthesis,genetics Chemotactic Factors/genetics Chemotaxis Cloning, Molecular Escherichia coli/genetics,physiology Escherichia coli Proteins Genes, Bacterial Kinetics Molecular Weight Plasmids Restriction Mapping
Chemicals
Bacterial Proteins CheW protein, E coli Chemotactic Factors Escherichia coli Proteins CheW protein, Bacteria
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Sanders D A
Department of Biochemistry, University of California, Berkeley 94704.
Mendez B
Koshland D E
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.171.11.6271-6278.1989
Published
1989-11-00
Pages
6271-8
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC210499
Subset
IM
Grants
NIDDK NIH HHS · DK09765 · United States
External Links
PubMed source DOI: 10.1128/jb.171.11.6271-6278.1989 PMC Full Text View journal details
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