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PMID: 2684651 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Induction of a growth-phase-dependent promoter triggers transcription of bolA, an Escherichia coli morphogene.

The EMBO journal ·Vol. 8 ·No. 12 ·1989-12-01 ·Pages 3923-31

Aldea M, Garrido T, Hernández-Chico C, Vicente M, Kushner SR

Abstract

The bolA gene, which is involved in the morphogenetic pathways of Escherichia coli, was sequenced and two potential promoters were identified. Expression from promoter P1, proximal to the bolA structural gene is specifically induced during the transition to the stationary phase of growth. This promoter contains an unusual--10 region (CGGCTAGTA), which defines a new class of E. coli promoters necessary for the dramatic increase in the rate of synthesis of a large set of proteins during the cessation of logarithmic growth. This conclusion was confirmed by identifying two additional E. coli promoters and one plasmid promoter, which also were induced during the transition to the stationary phase of growth. Analysis of proteins produced during the exponential and stationary phases of growth in a bolA null mutant suggest a possible role for the BolA protein in the induction of the expression of penicillin-binding protein 6 (PBP6) in the transition to the stationary phase. Supporting this hypothesis is the presence of a putative DNA-binding domain within the bolA coding sequence.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/biosynthesis,genetics,isolation & purification Base Sequence DNA, Bacterial/genetics,metabolism Escherichia coli/genetics,growth & development Gene Expression Regulation, Bacterial Genes, Bacterial Lac Operon Molecular Sequence Data Mutation Promoter Regions, Genetic Restriction Mapping Transcription, Genetic
Chemicals
Bacterial Proteins DNA, Bacterial
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Aldea M
Department of Genetics, University of Georgia, Athens 30602.
Garrido T
Hernández-Chico C
Vicente M
Kushner S R
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1989-12-01
Pages
3923-31
Language
English
Region
England
NLM ID
8208664
PMCID
PMC402084
Subset
IM
Grants
NIGMS NIH HHS · GM27997 · United States
NIGMS NIH HHS · GM28760 · United States
External Links
PubMed source PMC Full Text View journal details
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