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PMID: 2685748 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Promoter selectivity of Escherichia coli RNA polymerase: omega factor is responsible for the ppGpp sensitivity.

Nucleic acids research ·Vol. 17 ·No. 21 ·1989-11-11 ·Pages 8755-65

Igarashi K, Fujita N, Ishihama A

Abstract

Transcription in vitro of stringently controlled Escherichia coli genes by purified RNA polymerase holoenzyme is inhibited by guanosine tetraphosphate (ppGpp). In order to examine possible role of omega factor in this ppGpp sensitivity, RNA polymerases with or without the omega factor were reconstituted and tested for their ppGpp sensitivity using an in vitro mixed transcription system. RNA polymerase lacking the omega factor was found virtually insensitive to ppGpp but the addition of a purified omega factor restored the ppGpp sensitivity of this omega-free RNA polymerase. These results raise a possibility that the omega factor is a regulatory protein of RNA polymerase and is involved in the ppGpp-mediated alteration of the promoter selectivity.

MeSH Terms
DNA-Directed RNA Polymerases/antagonists & inhibitors,genetics Electrophoresis, Polyacrylamide Gel Escherichia coli/enzymology Gene Expression Regulation, Enzymologic Guanine Nucleotides/pharmacology Guanosine Tetraphosphate/pharmacology Promoter Regions, Genetic Transcription, Genetic
Chemicals
Guanine Nucleotides Guanosine Tetraphosphate DNA-Directed RNA Polymerases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Igarashi K
Department of Molecular Genetics, National Institute of Genetics, Shizuoka, Japan.
Fujita N
Ishihama A
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/17.21.8755
Published
1989-11-11
Pages
8755-65
Language
English
Region
England
NLM ID
0411011
PMCID
PMC335041
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/17.21.8755 PMC Full Text View journal details
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