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PMID: 268622 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Distribution of 5-methylcytosine in chromatin.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 74 ·No. 7 ·1977-07-00 ·Pages 2725-8

Razin A, Cedar H

Abstract

The content of 5-methylcytosine in eukaryotic DNA was measured by mass spectrometry. Almost equal amounts of methylated cytosine were found in the DNA of various tissues of the chicken. When chromatin or nuclei were digested with micrococcal nuclease, 50% of the DNA was found to be nuclease resistant. In contrast to this, over 75% of the 5-methylcytosine was protected from nuclease digestion by chromatin proteins. These results suggest that 5-methylcytosine is nonrandomly distributed with respect to the nucleoproteins.

MeSH Terms
Animals Cattle Chickens Chromatin/metabolism,ultrastructure Cytosine Nucleotides/metabolism Deoxycytidine Monophosphate/metabolism Erythrocytes Mass Spectrometry Methylation Micrococcal Nuclease Protein Binding Thymus Gland
Chemicals
Chromatin Cytosine Nucleotides Deoxycytidine Monophosphate Micrococcal Nuclease
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Razin A
Cedar H
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29 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.74.7.2725
Published
1977-07-00
Pages
2725-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC431263
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.74.7.2725 PMC Full Text View journal details
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