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PMID: 2690821 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Development of insulin sensitivity in white adipose tissue during the suckling-weaning transition in the rat. Involvement of glucose transport and lipogenesis.

The Biochemical journal ·Vol. 264 ·No. 1 ·1989-11-15 ·Pages 217-22

Issad T, Ferré P, Pastor-Anglada M, Baudon MA, Girard J

Abstract

The changes of insulin responsiveness of white adipose tissue during the suckling-weaning transition in the rat were investigated in vitro on isolated adipocytes. Insulin binding, glucose transport and glucose metabolism in adipocytes from suckling rats and from rats weaned on to a high-carbohydrate (HC) or a high-fat (HF) diet were compared. Despite similar insulin binding, insulin-stimulated glucose transport rate is lower in adipocytes from suckling rats and HF-weaned rats than in adipocytes from HC-weaned rats. Moreover, whereas insulin markedly stimulates glucose metabolism in adipocytes from HC-weaned rats, glucose metabolism is totally unresponsive to insulin in adipocytes from suckling and HF-weaned rats. This insulin resistance is associated with a very low rate of lipogenesis and low activities of acetyl-CoA carboxylase, fatty acid synthase and pyruvate dehydrogenase.

MeSH Terms
Adipose Tissue/physiology Animals Animals, Suckling/physiology Dietary Fats/metabolism Glucose/metabolism In Vitro Techniques Insulin/metabolism,pharmacology Insulin Resistance Lipids/biosynthesis Monosaccharide Transport Proteins/metabolism Rats Rats, Inbred Strains Weaning
Chemicals
Dietary Fats Insulin Lipids Monosaccharide Transport Proteins Glucose
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Issad T
Centre de Recherche sur la Nutrition (CNRS), Meudon-Bellevue, France.
Ferré P
Pastor-Anglada M
Baudon M A
Girard J
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2640217
Published
1989-11-15
Pages
217-22
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1133566
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2640217 PMC Full Text View journal details
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