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PMID: 2692850 Published · ppublish English Journal Article

Two new loci that give rise to dominant omnipotent suppressors in Saccharomyces cerevisiae.

Current genetics ·Vol. 16 ·No. 5-6 ·1989-12-00 ·Pages 323-30

Ono B, Tanaka M, Awano I, Okamoto F, Satoh R, Yamagishi N, Ishino-Arao Y

Abstract

Ten dominant omnipotent suppressors of Saccharomyces cerevisiae, which were previously shown to be different from SUP46, have been examined. Nine are mapped in a region between lys5 and cyh2 on the left arm of chromosome VII. These suppressors, like SUP46, manifest sensitivity to increased temperature and the antibiotics paromomycin and hygromycin B. In addition, they have an identical action spectrum. These results strongly suggest that they are allelic to each other and they are designated SUP138. The tenth is mapped to a position between his1 and arg6 on the right arm of chromosome V. This suppressor, named SUP139, does not manifest temperature sensitivity nor antibiotic sensitivity. SUP139 and SUP138, which are clearly distinguished by means of action spectrum, act on much fewer nonsense mutations than SUP46. It is now clear that dominant omnipotent suppressors arising at a single locus are homogeneous and that their efficiency is locus-dependent. The order of efficiency is SUP46 greater than SUP138 greater than SUP139.

MeSH Terms
Alleles Anti-Bacterial Agents/pharmacology Chromosome Mapping Drug Resistance, Microbial/genetics Genes, Fungal Genetic Linkage Genotype Hygromycin B/pharmacology Paromomycin/pharmacology Saccharomyces cerevisiae/genetics Suppression, Genetic Temperature
Chemicals
Anti-Bacterial Agents Hygromycin B Paromomycin
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Ono B
Laboratory of Environmental Hygiene Chemistry, Faculty of Pharmaceutical Sciences, Okayama University, Japan.
Tanaka M
Awano I
Okamoto F
Satoh R
Yamagishi N
Ishino-Arao Y
References (32)
32 references, click to expand
  1. Isolation of the SUP45 omnipotent suppressor gene of Saccharomyces cerevisiae and characterization of its gene product.
    Mol Cell Biol. 1985 Apr;5(4):816-22 PMID: 3887137
  2. The allosuppressor gene SAL4 encodes a protein important for maintaining translational fidelity in Saccharomyces cerevisiae.
    Curr Genet. 1988 Dec;14 (6):537-43 PMID: 3072098
  3. Genetic mapping of two pairs of linked ribosomal protein genes in Saccharomyces cerevisiae.
    Curr Genet. 1987;11(6-7):445-50 PMID: 3329973
  4. Recessive nonsense-suppression in yeast: involvement of 60S ribosomal subunit.
    Mol Gen Genet. 1978 Jul 6;163(1):87-90 PMID: 355843
  5. Localization of possible functional domains in sup2 gene product of the yeast Saccharomyces cerevisiae.
    FEBS Lett. 1987 May 11;215(2):257-60 PMID: 3556215
  6. Genetic mapping of leucine-inserting UAA suppressors in Saccharomyces cerevisiae.
    Curr Genet. 1985 Mar;9(3):197-203 PMID: 24173554
  7. Genetic map of Saccharomyces cerevisiae.
    Microbiol Rev. 1980 Dec;44(4):519-71 PMID: 7010111
  8. Genetic properties of some amber-ochre supersuppressors in Saccharomyces cerevisiae.
    Mol Gen Genet. 1975;138(1):53-63 PMID: 1102924
  9. Altered ribosomal protein L29 in a cycloheximide-resistant strain of Saccharomyces cerevisiae.
    Curr Genet. 1980 Apr;1(3):177-83 PMID: 24189656
  10. Recessive suppression in yeast Saccharomyces cerevisiae is mediated by a ribosomal mutation.
    FEBS Lett. 1980 Feb 25;111(1):175-8 PMID: 6987083
  11. Altered 40 S ribosomal subunits in omnipotent suppressors of yeast.
    J Mol Biol. 1986 Mar 20;188(2):207-14 PMID: 3522920
  12. Nucleotide sequence of the SUP2 (SUP35) gene of Saccharomyces cerevisiae.
    Gene. 1988 Jun 15;66(1):45-54 PMID: 3047009
  13. Allosuppressors that enhance the efficiency of omnipotent suppressors in Saccharomyces cerevisiae.
    Genetics. 1987 Mar;115(3):451-60 PMID: 3552876
  14. Yeast omnipotent supressor SUP1 (SUP45): nucleotide sequence of the wildtype and a mutant gene.
    Nucleic Acids Res. 1986 Jul 11;14(13):5187-97 PMID: 3526282
  15. Suppressors in yeast.
    Curr Top Microbiol Immunol. 1974;64(0):1-47 PMID: 4602646
  16. Cloning, nucleotide sequence, and expression of one of two genes coding for yeast elongation factor 1 alpha.
    J Biol Chem. 1985 Mar 10;260(5):3090-6 PMID: 2982849
  17. Recessive super-suppression in yeast.
    Mol Gen Genet. 1974 Mar 14;129(2):105-21 PMID: 4598794
  18. Mutations in elongation factor EF-1 alpha affect the frequency of frameshifting and amino acid misincorporation in Saccharomyces cerevisiae.
    Genetics. 1988 Dec;120(4):923-34 PMID: 3066688
  19. Biochemical Mutants in the Smut Fungus Ustilago Maydis.
    Genetics. 1949 Sep;34(5):607-26 PMID: 17247336
  20. A yeast gene required for the G1-to-S transition encodes a protein containing an A-kinase target site and GTPase domain.
    EMBO J. 1988 Apr;7(4):1175-82 PMID: 2841115
  21. Serine-inserting UAA suppression mediated by yeast tRNASer.
    J Mol Biol. 1981 Aug 15;150(3):361-73 PMID: 6795356
  22. Patterns of Genetic and Phenotypic Suppression of lys2 Mutations in the Yeast SACCHAROMYCES CEREVISIAE.
    Genetics. 1979 Sep;93(1):67-79 PMID: 17248970
  23. Cycloheximide-resistant temperature-sensitive lethal mutations of Saccharomyces cerevisiae.
    Genetics. 1988 Jun;119(2):303-15 PMID: 3294103
  24. Polypeptide chain elongation factor 1 alpha (EF-1 alpha) from yeast: nucleotide sequence of one of the two genes for EF-1 alpha from Saccharomyces cerevisiae.
    EMBO J. 1984 Aug;3(8):1825-30 PMID: 6383821
  25. Identification of two genes coding for the translation elongation factor EF-1 alpha of S. cerevisiae.
    EMBO J. 1984 Dec 20;3(13):3311-5 PMID: 6396088
  26. A ribosome-dependent GTPase from yeast distinct from elongation factor 2.
    Proc Natl Acad Sci U S A. 1976 Jan;73(1):73-6 PMID: 174100
  27. Serine insertion caused by the ribosomal suppressor SUP46 in yeast.
    J Mol Biol. 1981 Apr 15;147(3):373-9 PMID: 6273576
  28. Altered ribosomal protein S11 from the SUP46 suppressor of yeast.
    J Mol Biol. 1981 Apr 15;147(3):391-7 PMID: 7031259
  29. Recessive nonsense suppressors in Saccharomyces cerevisiae: action spectra, complementation groups and map positions.
    Genetics. 1986 Oct;114(2):363-74 PMID: 3533713
  30. Misreading of the ribosomal suppressor SUP46 due to an altered 40 S subunit in yeast.
    J Mol Biol. 1981 Apr 15;147(3):381-90 PMID: 7031258
  31. Function of positive regulatory gene gal4 in the synthesis of galactose pathway enzymes in Saccharomyces cerevisiae: evidence that the GAL81 region codes for part of the gal4 protein.
    J Bacteriol. 1980 Feb;141(2):508-27 PMID: 6988385
  32. Omnipotent Suppressors Effective in psi Strains of SACCHAROMYCES CEREVISIAE: Recessiveness and Dominance.
    Genetics. 1984 Jun;107(2):219-30 PMID: 17246215
Article Info
Journal
Current genetics
Abbr.
Curr Genet
ISSN
0172-8083
DOI
10.1007/BF00340710
Published
1989-12-00
Pages
323-30
Language
English
Region
United States
NLM ID
8004904
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00340710 View journal details
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