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PMID: 3526282 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Yeast omnipotent supressor SUP1 (SUP45): nucleotide sequence of the wildtype and a mutant gene.

Nucleic acids research ·Vol. 14 ·No. 13 ·1986-07-11 ·Pages 5187-97

Breining P, Piepersberg W

Abstract

The primary structures of the yeast recessive omnipotent suppressor gene SUP1 (SUP45) and one of its mutant alleles (sup1-ts36) was determined. The gene codes for a protein of 49 kD. The mutant protein differs from the wildtype form in one amino acid residue (Ser instead of Leu) in the N-terminal part. The codon usage differs significantly from that of yeast ribosomal protein genes. However, an upstream element resembling a conserved oligonucleotide in the region 5' to ribosomal protein genes in S. cerevisiae has been found. A DNA probe internal to the SUP1 gene does not exhibit detectable homology to genomic DNA neither from higher eucaryotes nor from eu- or archaebacteria. The hypothetical function of this protein in control of translational fidelity is discussed.

MeSH Terms
Amino Acid Sequence Amino Acyl-tRNA Synthetases/genetics Base Sequence Cloning, Molecular Codon Fungal Proteins/genetics Mutation Ribosomal Proteins/genetics Saccharomyces cerevisiae/genetics Suppression, Genetic
Chemicals
Codon Fungal Proteins Ribosomal Proteins Amino Acyl-tRNA Synthetases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Breining P
Piepersberg W
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24 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/14.13.5187
Published
1986-07-11
Pages
5187-97
Language
English
Region
England
NLM ID
0411011
PMCID
PMC311534
Subset
IM
Databases
GENBANK
X04082
External Links
PubMed source DOI: 10.1093/nar/14.13.5187 PMC Full Text View journal details
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